The CHROMacademy Essential Guide Webcast: Optimizing API Interfaces
Thursday 18th December 2014,
8:00am PDT / 11:00am EDT / 16:00 BST / 17:00 CEST
Many API interface parameters are set and never optimized to the detriment of the analysis. The fundamentals of the ionization processes involved in LC-MS will be detailed along with a discussion of the major components of ESI and APCI interfaces. We will look at the optimization of API interfaces and the effect that they can have on analytical results. Ion suppression can be an insidious problem which leads to poor sensitivity, understanding the causes of ion suppression will allow this problem to be remedied or avoided completely.
Presented by Christine Gu (xxx, Genentech) and Tony Taylor (Technical Director, Crawford Scientific).
Process of ionization in LC-MS
What are the major interface parameters that require optimization in ESI and APCI
In ESI what effect will sprayer voltage and position, drying gas temperature and flow rates have on analyses
Optimization of the major APCI interface parameters - vaporizing probe temperature, curtain gas, and nebulizer gas
How do you select the correct ionization modes
Tune vs. calibration for successful analyses
Causes of and remedies for ion suppression
Key Learning Objectives:
Understand the function of each of the major API interface components
Learn which API parameters should be optimized for ideal analytical results
Know how to select the correct ionization mode for you analytes
Gain knowledge on avoiding ion suppression resulting in maximum analytical sesnsitivity
Who Should Attend:
Anyone working with LC
Anyone working with LC-MS and wants to get the best performance from their equipment
Anyone who wants to improve their knowledge of LC-MS
Find out more about this Month's Essential Guide Webcast »
Optimizing API Interface Parameters Tutorial - December 2014
Many API interface parameters are set and never optimized to the detriment of the analysis. The fundamentals of the ionization processes involved in LC-MS will be detailed along with a discussion of the major components of ESI and APCI interfaces. We will look at the optimization of API interfaces and the effect that they can have on analytical results.
Ion suppression can be an insidious problem which leads to poor sensitivity, understanding the causes of ion suppression
will allow this problem to be remedied or avoided completely.
Watch the webcast and delve into our CHROMacademy material to further your knowledge of API interface parameters.
A comprehensive module which introduces the mechanism or electrospray ionization (ESI), presents the main theories which explain ion production processes, and indicates when the technique should be used as well as its advantages and limitations.
This CHROMacademy module will allow you to understand the instrumentation associated with electrospray ionization by explaining the function of the major instrument components and how there can be used to optimize the MS signal.
This module introduces the main concepts of atmospheric pressure chemical ionization (APCI) as well as explaining the function of each major component within the APCI interface. Optimization and troubleshooting of this technique are covered alongside the major advantages of using APCI.
Here are 10 great tips for optimizing LC-ESI-MS so that you achieve the best possible results every time. The tips and tricks include everything from which API interface parameters to optimize to which solvents produce the best ionization.
Instrument manufacturers try to convince us that mass spec is just another detector. Most of us who work with LC-MS know that’s simply not the case – they can be maintenance intensive, unforgiving and generate complex information. When they’re not working it can be difficult to work out exactly where the problem lies. Here’s some advice to point you in the right direction.
If there’s one mantra that’s essential for an LC (or GC)-MS operator it’s “contamination, contamination, contamination”. Keep contamination to a minimum and you’ll enjoy a reliable instrument; don’t take the necessary precautions and instead you’ll enjoy reaching for the vent switch and purchasing lots of spares.
In this session Arindam Roy (Associate Director, Quality, Novartis Consumer Health) and Scott Fletcher (Technical Manager, Crawford Scientific) will consider a range of critical LC-MS method parameters and their impact on analytical sensitivity and specificity. The importance and impact of specific eluent chemistry, interface parameters, analyzer settings and types are all critically reviewed and evaluated in this highly relevant presentation.
In this session we consider the major instrument and method variables in LC-MS analysis as well as some of the more minor ones which can sometimes make a big difference to your analysis. Eluent chemistry, interface parameters and analyzer settings are all considered in a lively discussion format where you will have the chance to ask questions and get answers.
This month our industry experts help you to understand the fundamental basis of electrospray ionization mass spectrometry. Learn how to optimize the essential parameters and improve instrument response and reproducibility in your lab. Improve your electrospray data and troubleshoot your ionization and data interpretation problems more effectively. Learn the fundamental basis of ion suppression so you can take best steps to avoid this phenomenon and discover best practice for acquiring qualitative data.