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The CHROMacademy Essential Guide Webcast:
Optimizing API Interfaces


Thursday 18th December 2014,
8:00am PDT / 11:00am EDT / 16:00 BST / 17:00 CEST

Many API interface parameters are set and never optimized to the detriment of the analysis. The fundamentals of the ionization processes involved in LC-MS will be detailed along with a discussion of the major components of ESI and APCI interfaces. We will look at the optimization of API interfaces and the effect that they can have on analytical results. Ion suppression can be an insidious problem which leads to poor sensitivity, understanding the causes of ion suppression will allow this problem to be remedied or avoided completely.

Presented by Christine Gu (xxx, Genentech) and Tony Taylor (Technical Director, Crawford Scientific).

  • Process of ionization in LC-MS
  • What are the major interface parameters that require optimization in ESI and APCI
  • In ESI what effect will sprayer voltage and position, drying gas temperature and flow rates have on analyses
  • Optimization of the major APCI interface parameters - vaporizing probe temperature, curtain gas, and nebulizer gas
  • How do you select the correct ionization modes
  • Tune vs. calibration for successful analyses
  • Causes of and remedies for ion suppression

Key Learning Objectives:

  • Understand the function of each of the major API interface components
  • Learn which API parameters should be optimized for ideal analytical results
  • Know how to select the correct ionization mode for you analytes
  • Gain knowledge on avoiding ion suppression resulting in maximum analytical sesnsitivity

Who Should Attend:

  • Anyone working with LC
  • Anyone working with LC-MS and wants to get the best performance from their equipment
  • Anyone who wants to improve their knowledge of LC-MS
 
Optimizing API Interfaces
 

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Find out more about this Month's Essential Guide Webcast »

 
 

ThermoOptimizing API Interface Parameters Tutorial - December 2014

Many API interface parameters are set and never optimized to the detriment of the analysis.  The fundamentals of the ionization processes involved in LC-MS will be detailed along with a discussion of the major components of ESI and APCI interfaces.  We will look at the optimization of API interfaces and the effect that they can have on analytical results. 

Ion suppression can be an insidious problem which leads to poor sensitivity, understanding the causes of ion suppression
will allow this problem to be remedied or avoided completely.
Watch the webcast and delve into our CHROMacademy material to further your knowledge of API interface parameters.

Share this tutorial

eLearning Modules

 
Electrospray Ionization Theory

A comprehensive module which introduces the mechanism or electrospray ionization (ESI), presents the main theories which explain ion production processes, and indicates when the technique should be used as well as its advantages and limitations.

Production of Charged Droplets at the Capillary Tip | Formation of the Taylor Cone |
Nebulisation - Applied Potential Difference
| Ion Suppression in Practice

 
Electrospray Ionization Instrumentation

This CHROMacademy module will allow you to understand the instrumentation associated with electrospray ionization by explaining the function of the major instrument components and how there can be used to optimize the MS signal.

Electrospray Source Design | Source Heating (Drying Gas) | Optimzing Sprayer / Sampling Plate Configuration |
Curtain Gas Systems to Prevent Cluster Ions

 
Atmospheric Pressure Chemical Ionization (APCI)

This module introduces the main concepts of atmospheric pressure chemical ionization (APCI) as well as explaining the function of each major component within the APCI interface.  Optimization and troubleshooting of this technique are covered alongside the major advantages of using APCI.

APCI Nebuliser Types | APCI Nebuliser Gas Flow | Eluent Additives in APCI | APCI Source Parameter Optimization

 
Quick Guides
 
LC-ESI-MS Results

Here are 10 great tips for optimizing LC-ESI-MS so that you achieve the best possible results every time.  The tips and tricks include everything from which API interface parameters to optimize to which solvents produce the best ionization.

LC-ESI-MS Results

 
Avoiding LC-MS Signal Suppression

Some practical insights into optimizing LC-MS eluents and parameters to avoid signal suppression.

Avoiding LC-MS Signal Suppression

 
Does Mass Spectrometry Need Chromatography?

Read on to find out what Professor Kevin Schug (University of Texas at Arlington).

Does Mass Spectrometry Need Chromatography?

 
My LC-MS Isn’t Behaving! Where Do I Start?

Instrument manufacturers try to convince us that mass spec is just another detector. Most of us who work with LC-MS know that’s simply not the case – they can be maintenance intensive, unforgiving and generate complex information. When they’re not working it can be difficult to work out exactly where the problem lies. Here’s some advice to point you in the right direction.

My LC-MS Isn’t Behaving! Where Do I Start?

 
7 Must Know Points to Understand Atmospheric Pressure Chemical Ionization

CHROMacademy delivers key knowledge quickly in bite-size modules, and to prove this here is our 7 point guide to understanding atmospheric pressure chemical ionization.

7 Must Know Points to Understand Atmospheric Pressure Chemical Ionization

 
10 Ways to Break Your LC-MS

If there’s one mantra that’s essential for an LC (or GC)-MS operator it’s “contamination, contamination, contamination”. Keep contamination to a minimum and you’ll enjoy a reliable instrument; don’t take the necessary precautions and instead you’ll enjoy reaching for the vent switch and purchasing lots of spares.

10 Ways to Break Your LC-MS

 
10 Must Know Points to Understand Electrospray Ionization

The title says it all.  Read on for our 10 must know points.

10 Must Know Points to Understand Electrospray Ionization

 
LC-MS Method Parameters - Critical Evaluation

In this session Arindam Roy (Associate Director, Quality, Novartis Consumer Health) and Scott Fletcher (Technical Manager, Crawford Scientific) will consider a range of critical LC-MS method parameters and their impact on analytical sensitivity and specificity. The importance and impact of specific eluent chemistry, interface parameters, analyzer settings and types are all critically reviewed and evaluated in this highly relevant presentation.

LC-MS Method Parameters - Critical Evaluation

 
Optimizing LC-MS and LC-MS/MS

In this session we consider the major instrument and method variables in LC-MS analysis as well as some of the more minor ones which can sometimes make a big difference to your analysis. Eluent chemistry, interface parameters and analyzer settings are all considered in a lively discussion format where you will have the chance to ask questions and get answers.

Optimizing LC-MS and LC-MS/MS

 
Electrospray Ionization (ESI) for LC-MS

This month our industry experts help you to understand the fundamental basis of electrospray ionization mass spectrometry.  Learn how to optimize the essential parameters and improve instrument response and reproducibility in your lab.  Improve your electrospray data and troubleshoot your ionization and data interpretation problems more effectively.  Learn the fundamental basis of ion suppression so you can take best steps to avoid this phenomenon and discover best practice for acquiring qualitative data.

Electrospray Ionization (ESI) for LC-MS

 
 
 
 
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  • Process of ionization in LC-MS
  • What are the major interface parameters that require optimization in ESI and APCI
  • In ESI what effect will sprayer voltage and position, drying gas temperature and flow rates have on analyses
  • Optimization of the major APCI interface parameters - vaporizing probe temperature, curtain gas, and nebulizer gas
  • How do you select the correct ionization modes
  • Tune vs. calibration for successful analyses
  • Causes of and remedies for ion suppression

Who Should Attend:

  • Anyone working with LC
  • Anyone working with LC-MS and wants to get the best performance from their equipment
  • Anyone who wants to improve their knowledge of LC-MS

 

Tony Taylor
Technical Director
Crawford Scientific

Dr. Christine Gu
Scientist, Department of Small Molecule Pharmaceutical Science
Genentech