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The CHROMacademy Essential Guide Webcast:
UV or MS, Derivatized or Not? Choosing and Optimizing a Workflow for Amino Acid Analysis in Spent Media

North America: Thursday 22nd February 2018,
11:00am EST/ 10:00am CST/ 8:00am PST

Europe: Friday 23rd February 2018,
9:00am GMT/ 10:00am CET

Asia Pacific: Friday 23rd February 2018,
8:00am IST/ 10:30am CST/ 11:30am JST


 

Agilent

 

This webcast will discuss workflow options for spent media analysis, focusing on LC/UV and LC/MS methods.  Guidance will be given for which workflow to choose depending on your needs and priorities.  Additionally, tips to get the best data from each approach will be discussed.

Presented by

Dr. Anne Blackwell (Product Support Scientist, Agilent Technologies)
Dr. Jordy Hsiao (R&D Scientist, Agilent Technologies)

Topics Covered

  • Choosing a workflow for spent media analysis that meets your needs
  • Benefits of using LC/UV or LC/MS for amino acid analysis
  • Examples of other metabolites that can be monitored simultaneously with amino acids
  • Advice for optimizing whichever approach you choose

Presenter Information »

 
 

UV or MS, Derivatized or Not? Choosing and Optimizing a Workflow for Amino Acid Analysis in Spent Media.

This month’s webcast focused on the analysis of amino acids in spent media.  The CHROMacademy material provided in this month’s tutorial provide you with even more information on amino acid analysis in relation to biopharmaceuticals.
Our Quick guides, CHROMacademy course content, and archived webcasts and tutorials fundamental knowledge and practical information relating to amino acid analysis.

Watch the webcast

eLearning Modules


 
Amino Acids, Peptides, and Proteins

This module provides an introduction to the primary constituents of biotherapeutic proteins.

amino acids peptides and proteins | amino acids in protein synthesis
 
Introduction to Biopharmaceutical Analysis

This module details the primary chromatographic techniques which are used to characterize biopharmaceuticals at different levels; including protein, peptide, and amino acid levels.

Introduction and Benefits (Reversed Phase HPLC) | Amino Acid Level Analysis by Reversed Phase HPLC
 

Quick Guides


Introduction to Amino Acid Analysis in Biopharmaceuticals

An introduction to the challenges of analyzing amino acids at different stages of biopharmaceutical synthesis.

Introduction to Amino Acid Analysis in Biopharmaceuticals »

 

HILIC, IEX, and SEC for the Analysis of Biomolecules

Biopharmaceuticals offer great hope in treating medical conditions which are currently poorly served at best by traditional pharmaceuticals. It is estimated that there are over 400 biopharmaceuticals in clinical trials for in excess of 200 disease areas. The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing. 

HILIC, IEX, and SEC for the Analysis of Biomolecules »

 
Choosing the Correct Column & Mobile Phase for LC-MS Characterization of Biologics

This webcast will focus on the parameters which are essential for the development of a successful RPLC-MS method for the characterization of a monoclonal antibody.  The methods will allow for the analysis of the intact species, determination of absolute mass, examination of the major fragments, (Fab/Fc and Hc/Lc), and investigation of the small peptides produced by protease digestion.  The importance of pore size and mobile phase additives will be addressed and guidance given on how to optimize methods for sensitivity and resolution.

Choosing the Correct Column & Mobile Phase for LC MS Characterization of Biologics »

 
HPLC Techniques in Biopharmaceutical Analysis

Biopharmaceuticals offer great hope in treating medical conditions which are currently poorly served at best by traditional pharmaceuticals.  It is estimated that there are over 400 biopharmaceuticals in clinical trials for in excess of 200 disease areas.  The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing.  This Essential Guide webcast will provide details of why differing modes of chromatography are required in the research, preparation and analysis of biopharmaceuticals and when they would be typically used.

HPLC Techniques in Biopharmaceutical Analysis »

 
 
 
 
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Dr. Anne Blackwell
Product Support Scientist
Agilent Technologies

Anne joined Agilent Technologies in 2012 after completing her PhD in mass spectrometry of non-covalent protein assemblies at the University of Arizona. 

She worked as an LC/MS applications chemist on metabolomics and ion mobility workflows until she joined the BioColumns Applications Team as a Product Support Scientist in 2016. 

Anne specializes in helping customers develop robust biomolecule separations, implement best practices to avoid common problems, and troubleshoot challenging problems when they arise.


Dr. Jordy Hsiao
R&D Scientist
Agilent Technologies

Jordy joined Agilent Technologies after completing his Ph.D. training in Dr. Michael Wright’s lab at the University of Iowa in 2015.

His study focused on adapting quantitative proteomics to study the androgen receptor interactome in prostate cancer cells, which allowed him to gain extensive technical expertise in protein biochemistry, analytical chemistry, and LC/MS experiments.

Since he joined Agilent, he has developed a host cell protein (HCP) workflow aimed to accelerate the comprehensive characterization of low level contaminant proteins present in purified biopharmaceutical products. Recently, he has been working on metabolomics applications using HILIC-LC/MS.