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Biochromatography Modules Developed with The Research Institute for Chromatography  
 
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Advances in Full Characterization of Protein Biopharmaceutical Post Translational Modifications Advances in Full Characterization of Protein Biopharmaceutical Post Translational Modifications

Post translational modification (PTMs) which are most characteristic in protein biopharmaceuticals include glycosylation (which comprises galactosylation, fucosylation, high mannose derivatives and sialylation), oxidation, phosphorylation, sulfation, lipidation, disulfide bond formation and deamidation, as well as... View Video Intro here »

Advances in Full Characterization of Protein Biopharmaceutical Post Translational Modifications »

 
Techniques Employed in Biopharmaceutical Analysis Techniques Employed in Biopharmaceutical Analysis - Part I Reversed Phase and HILIC

This webcast shall interrogate the critical parameters required to succesfully implement and optimize reversed phase analysis of biopharmaceuticals. We will also discuss the popularity behind glycosylated biopharmaceuticals, where heterogeneity arises in these molecules, and how glycans can be effectively liberated, characterized, and analyzed using HILIC.

Techniques Employed in Biopharmaceutical Analysis - Part I Reversed Phase and HILIC »

 
Techniques Employed in Biopharmaceutical Analysis Techniques Employed in Biopharmaceutical Analysis - Part II Ion Exchange and SEC

Ion exchange (IEX) and size exclusion chromatography (SEC) provide much more information than merely charge variance and aggregation analysis. In this event we will discuss how IEX and SEC are typically employed in biopharmaceutical characterization and quantification, highlight strategies for developing more robust methods, consider the effect of temperature and pressure on SEC separations, and the role of UHPLC. Finally an introduction to hydrophobic interaction chromatography (HIC) will also be given.

Techniques Employed in Biopharmaceutical Analysis - Part II Ion Exchange and SEC »

 
HPLC Techniques in Biopharmaceutical Analysis HPLC Techniques in Biopharmaceutical Analysis

The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing. This Essential Guide webcast will provide details of why differing modes of chromatography are required in the research, preparation, and analysis of biopharmaceuticals and when they would be typically used.

HPLC Techniques in Biopharmaceutical Analysis »

 
Characterization of Protein Biopharmaceuticals Characterization of Protein Biopharmaceuticals

Protein biopharmaceuticals, such as monoclonal antibodies and recombinant proteins, are currently in widespread use for treatment of various life-threatening diseases including cancer, autoimmune diseases, diabetes, anemia, etc. Protein therapeutics have a complexity far exceeding that of small molecule drugs, hence, unraveling this complexity represents an analytical challenge. This webcast will highlight the power of liquid chromatography combined with mass spectrometry in the characterization of protein biopharmaceuticals.

Characterization of Protein Biopharmaceuticals »

 
The Analysis of Post Translational Modifications Using LC-MS/MS The Analysis of Post Translational Modifications Using LC-MS/MS

This webcast will explain the principles behind the analysis of a number of key post-translational modifications (PTMs) using LC-MS/MS techniques. The use of bottom-up proteomics and different MS scanning techniques to characterize PTMs such as, phosphorylation, acetylation, ubiquitination and SUMOylation will be covered.

The Analysis of Post Translational Modifications Using LC-MS/MS »

 
Reversed Phase HPLC for the Analysis of Biomolecules Reversed Phase HPLC for the Analysis of Biomolecules.

Commonly reversed phase (RP), hydrophilic interaction (HILIC), ion exchange (IEX), and size exclusion (SEC) chromatographic modes will be combined to allow full characterization of complex biomolecules. The following article will introduce the fundamentals of biopharmaceutical analysis and cover the use of reversed phase HPLC in their analysis.

Reversed Phase HPLC for the Analysis of Biomolecules »

 
HILIC, IEX, and SEC for the Analysis of Biomolecules HILIC, IEX, and SEC for the Analysis of Biomolecules

The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing. This article will examine the use of HILIC, IEX, and SEC for their full characterization.

HILIC, IEX, and SEC for the Analysis of Biomolecules »

 
Quick Guide Biomolecules Selecting Columns and Initial Conditions for IEX and SEC

Biopharmaceutical compounds are genetically engineered from living cells and have molecular weights in the range 2,000-2,000,000 Da with 10-2,000 reactive groups and are often comprised of a mixture of closely related variants; therefore, there is a wider degree of variability (a degree of heterogeneity) in comparison with small molecule pharmaceuticals. 

Selecting Columns and Initial Conditions for IEX and SEC »

 

Agilent AdvanceBio column portfolio: enhance the accuracy and speed of your biomolecule characterization

Introducing Agilent AdvanceBio SEC Columns

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Biochromatography from CHROMacademy

Protein biopharmaceuticals such as monoclonal antibodies and recombinant proteins are currently in widespread use for the treatment of various life-threatening diseases including cancer and autoimmune diseases. Protein therapeutics are large and heterogeneous and as a result have a complexity far exceeding that of small-molecule drugs. Unraveling this complexity represents an analytical challenge.

Welcome to the Biochromatography department
Dwight R. Stoll  Assistant Professor of Chemistry  Gustavus Adolphus College

It is with great excitement that we present to you this biochromatography module. The module has been designed to provide insight in the strategies to tackle this analytical challenge. The information provided is the result of knowledge and knowhow gained through years of collaborations with the pharma and biotech industry. Read and study the material but above all enjoy as much as we have enjoyed preparing this for you.

Koen Sandra
Scientific Director
Research Institute for Chromatography
www.richrom.com
R&D Director
anaRIC biologics
www.anaricbiologics.com

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Welcome to the Biochromatography department

Dwight R. Stoll  Assistant Professor of Chemistry  Gustavus Adolphus College  It is with great excitement that we present to you this biochromatography module. The module has been designed to provide insight in the strategies to tackle this analytical challenge. The information provided is the result of knowledge and knowhow gained through years of collaborations with the pharma and biotech industry. Read and study the material but above all enjoy as much as we have enjoyed preparing this for you.

About RIC

The Research Institute for Chromatography (RIC) is an established SME founded in 1986 by Prof. Pat Sandra. The company provides chromatographic, electrophoretic and mass spectrometric support in a broad range of application areas. RIC supports the chemical, pharmaceutical and life sciences industries by means of analytical R&D (method development/validation, sample analysis, instrument development, etc.), training, consultancy, distribution of analytical instrumentation and by providing total analytical solutions.

Koen Sandra
Scientific Director
Research Institute for Chromatography
www.richrom.com
R&D Director
anaRIC biologics
www.anaricbiologics.com