Column dimensions, temperature programming, introduction to GC detectors
In this session we investigate the important choices which govern the choice of GC column dimensions and how to optimize efficiency and analysis time.
We also discuss sample screening methods to investigate the retention behaviour of the analytes and make some initial decisions regarding the requirements for the temperature program.
Using temperature programming theory, we investigate how to optimize the gradient parameters such as initial and final temperature and the slope of the temperature gradient. We discuss the use of mid-ramp holds for separating critical peak pairs within the analysis. We also begin our discussion on GC detectors by examining the range of detector types available and the factors which govern detector selection.
Tony has been doing, researching, teaching and training in analytical chemistry for the past 28 years. He comes from a pharmaceutical and polymer analysis background and continues to work with both liquid and gas phase techniques at Crawford Scientific (UK).
His main interests are the use of LC-MS and GC-MS for structural characterisation and the quantitation of trace components in complex matrices. He is professionally qualified trainer and is Technical Director of the CHROMacademy.
Associated Training Material from CHROMacademy
FAQs will be added as students progress through the course.