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The CHROMacademy Essential Guide Webcast:
What GC Operators Need to Know.
Part 1: From Gas Supply and Inlets to Column Selection and Temperature Programming.

Thursday 18th June 2015,
8:00am PDT / 11:00am EDT / 16:00 BST / 17:00 CEST

During this two part event we will look at the whole GC process from sampling, to injection, separation, and detection. We will provide practical hints and tips for the essential GC parameters in order to make your use of this powerful technique straightforward and successful. Firstly we will discuss the gases required for GC, critical choices in gas sampling and sample preparation, and how to optimize your sample injection routine. Column selection is probably one of the most important choices in any chromatographic method; therefore, we will share our insights on how to select the correct column each time. Finally, we will detail how to utilize and optimize isothermal and temperature programmed methods to produce optimum resolution.

Presented by John Hinshaw (Senior Scientist, BPL Global Ltd) and
Tony Taylor (Technical Director, Crawford Scientific).

Gas Supply and Quality
  • Cylinders and generators
  • Correct use of gas filters for cleaning carrier and detector gases
  • Gas requirements for detectors
Sampling and Sample Preparation
  • What needs to be considered when choosing a sampling technique?
  • The importance of the correct sample preparation technique -
    from solvent choice to dealing with complex samples
Inlet Considerations
  • Which inlet for which analysis
  • How to avoid the common inlet pitfalls
  • Getting the most out of your chosen sample introduction technique
GC Column Selection
  • GC column selection is critical to obtaining the correct separation selectivity –
    what factors influence stationary phase choice and dimensions?
  • Whilst choosing a ‘generic’ phase can bring satisfactory results, there are often better choices which will improve specificity and sensitivity
  • Appreciate when to stop optimizing method conditions and start choosing a different column
  • How to correctly install a column
GC Temperature Programming
  • The effect of temperature on analyte migration
  • What can a scouting gradient tell us?
  • Predicting the optimum isothermal conditions
  • Predicting the optimum temperature programming conditions, including, initial temperature, use of initial hold, the optimum ramp rate, and when and at what
    temperature a GC temperature program should end

Key Learning Objectives:

  • Understand the GC process and which parameters can and should be considered
  • Learn how to select the appropriate sample preparation technique for your sample
  • Learn how to make the most of your inlet and avoid common pitfalls
  • Gain knowledge on GC column stationary phases and dimensions
  • Understand the differences between isothermal and temperature programmed conditions

Who Should Attend:

  • All analytical scientists
  • Anyone using GC
 
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Find out more about this Month's Essential Guide Webcast »

 
 

What GC Operators Need to Know (Part I)

Every GC operator should have certain knowledge and skills in order to produce the right quality data in the most efficient manner.  This series of two webcasts highlights this knowledge and provides a very pragmatic guide to the day to day operation of a Gas Chromatograph as well as a valuable insight into the important variables from any GC method.

Every GC needs to be set-up properly prior to analysis, which may include checks on supply gas checks, column installation and conditioning as well as routine inlet and detector maintenance checks.  A checklist of these items is good to follow and is often included in an instrument operation SOP – but do we always follow the SOP least a rudimentary?  When was the last time you questioned WHY, some of these operations are necessary?

The instrument then needs to be equilibrated and test injections made to assess the suitability of the system to produce valid analytical data.  The baseline, chromatographic appearance and any quantitative output should be evaluated for suitability.  What is the best way to do this?

Every GC operator should be able to critically evaluate the method being implemented with a rudimentary knowledge of the column chemistry and why this particular column stationary phase and dimensions have been chosen for the particular analysis.  The method of sample preparation should be understood and the choice of sample solvent should be rationalised against the conditions at the beginning of the analysis.  The inlet conditions should be checked to ensure they are appropriate for the analysis and do not risk problems with system contamination or poor quantitative reproducibility.
The oven temperature program should be rationalised against the required separation and sample complexity and the detector type and settings should be checked to ensure they are appropriate.

Only by having the ability to properly implement an analysis and critically evaluate the method under consideration can a GC operator produce data which is fit for purpose on an on-going basis and begin to investigate any problems which might arise?
Are YOU fit for purpose?  This series of webcasts will help to ensure that you are!

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Fundamentals of GC - Video Training Course Fundamentals of GC - Video Training Course

This four session training course contains everything you need to know in order to become a highly proficient GC operator and gain a deep understanding of the technique.  The instrumentation, theory and application of GC is considered in great detail and this course is highly recommended. 

The course is assessed in order that you can prove competence in this important analytical technique.
The course requires registration and will run on four consecutive Thursdays from July 16th.  Each session is recorded and can be viewed ‘on-demand’ if you cannot make the scheduled broadcast times.  There are a limited number of seats available so each attendee is expected to complete each module and the final assessment.

GC Course Overview   View course descriptions and watch sample video
 

eLearning Modules


GC Detectors Sample Introduction

Often described as the ‘Achilles Heel’ of Gas Chromatography – it’s important to get your inlet operating mode and settings correct.  In order to introduce a homogenous gaseous sample which is representative of the sample and does not cause band broadening (loss of efficiency) is the name of the game and some knowledge of the operating principles and correct settings for various inlet modes is vital.

Split / Splitless | Split Injection | Setting the Split Ratio | Sample Discrimination

 
GC Detectors GC Detectors

Choosing the appropriate detector, knowing what sensitivity and linearity to expect and understanding how to optimise detector output is critical for any GC Operator.  A good understanding of the operating principles and variable setting is important and you should be able to critically evaluate any method you are using and rationalise the settings against the application being implemented.

The Flame Ionisation Detector | FID Operating and Optimising | FID Uses and Performance

 
 
Quick Guides

Fast HPLC Crimes Against GC Gas Filters

Are you guilty of any of our most commonly committed ‘crimes’ against GC gas filters?

Crimes Against GC Gas Filters »

 
Fast HPLC Best Practice for GC Sample Introduction

No matter what kind of GC inlet you are using we have some helpful hints that can be applied to achieve the best sample introduction every time.

Best Practice for GC Sample Introduction »

 
Fast HPLC Quick Guide to Capillary GC Stationary Phases

The single biggest factor in obtaining a suitable GC separation is the choice of column chemistry. Get it right and everything moves smoothly, get it wrong and you’ll waste a lot of time trying to optimize all the wrong parameters before having to buy another column anyway.

Quick Guide to Capillary GC Stationary Phases »

 
Fast HPLC GC Column Installation and Conditioning Guide - Video

A properly installed GC column is the most important beginning for your work. This 5 minute Quick Guide video demonstrates how to properly install a GC column, giving you great chromatography, analysis after analysis.

GC Column Installation and Conditioning Guide - Video »

 
Fast HPLC GC Temperature Programming - 10 Things You Absolutely Need to Know

Why does the oven temperature in splitless analysis always start at a low temperature?
What is the optimum initial hold time for any GC temperature program?
Should I be running the separation in Isothermal mode?
What is the optimum temperature ramp rate for a separation?
Find out this and much more here…

GC Temperature Programming - 10 Things You Absolutely Need to Know »

 
GC Detectors Column Selection for Capillary GC

The title says it all.  After this webcast you will understand capillary GC stationary phase chemistry and understand why certain stationary phases are chosen for particular applications.  You will know how to fine tune the stationary phase chemistry and how the selectivity changes depending upon the analyte characteristics.  You will learn the principles of selecting column length, internal diameter and film thickness based on the nature of the sample and analytes under investigation.

Column Selection for Capillary GC »

 
GC Detectors GC Troubleshooting - Gas Supply and Inlet Issues

For those who wish to understand more in how to spot and diagnose problems with GC gas supply and sample introduction (two critical areas for GC operators) this webcast has the key information you require.  Useful information on ensuring high quality gas is supply and the classic symptoms to look out for when things are going wrong is supported by hints and tips on how to avoid trouble or how to fix issues if they do arise.  All of the common problems with GC Inlets are explored, again with illustrative examples and information on how to avoid or fix problems.

GC Troubleshooting - Gas Supply and Inlet Issues »

 
 
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Key Learning Objectives:

  • Understand the GC process and which parameters can and should be considered
  • Learn how to select the appropriate sample preparation technique for your sample
  • Learn how to make the most of your inlet and avoid common pitfalls
  • Gain knowledge on GC column stationary phases and dimensions
  • Understand the differences between isothermal and temperature programmed conditions

Who Should Attend:

  • All analytical scientists
  • Anyone using GC

John V Hinshaw
Senior Scientist
BPL Global Ltd

 

Tony Taylor
Technical Director
Crawford Scientific