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The CHROMacademy Essential Guide Webcast:
GC Troubleshooting in 20 Pictures - Part 2
 

Thursday 17th December 2015,
8:00am PDT / 11:00am EDT / 16:00 BST / 17:00 CEST

A picture paints a thousand words…

The art of GC troubleshooting often lies in being able to recognize a problem from the evidence presented in the chromatogram or baseline appearance.

Real life chromatograms and baselines will be used to illustrate key issues with Gas Chromatography including;

  • Consequences of impure carrier and detector gases
  • Improperly designed Sample Introduction methods
  • Poorly maintained sample inlets
  • Badly calculated sample loading
  • Changes in separation selectivity
  • Dealing with difficult compounds
  • Poorly installed columns
  • Insidious detector issues

Of course we will also let you know how to fix these problems or better still, avoid them in the first place.

Presented by Tony Taylor (Technical Director, Crawford Scientific).


Key Learning Objectives:

  • Recognize key GC issues from the appearance of the chromatogram or baseline
  • Appreciate the requirements for high quality gas supplies and understand how to achieve the optimum in gas cleanliness
  • Understand the optimal way to configure a split / splitless injection system and the routine maintenance and checks to keep it working properly
  • Understanding column loading and designing appropriate analyses to avoid column overload
  • Understand the principles of column installation and conditioning
  • Investigating issues with column and analyte chemistry
  • Recognize issues with settings or gas supply for common GC Detectors

Who Should Attend:

  • Anyone using GC
  • Anyone wishing to improve their GC troubleshooting skills
 
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Find out more about this Month's Essential Guide Webcast »

 
 

GC Troubleshooting in Pictures Part 2- Tutorial

They say a picture paints a thousand words…

In this second instalment of the two part series we present further real data from our work, and those of the CHROMacademy members, that represents some ‘classic’ GC problems.

The ability to recognise baseline and separation problems and identify their causes from the everyday pictures we see on our data systems, is a fundamental skill that every chromatographer should learn.

The second 10 of these is presented below, with a brief explanation of the potential cause and suggested fixes.
You can use these to recognise similar problems in your own work – and build your ‘I’ve seen that before…….’ experience!

A simple test mix was used to reconstruct several of these problems and the test mix components and basic GC conditions were;

2,3-Butanediol Methyl decanoate
Decane Methyl laurate    
Dicyclohexylamine Methyl undecanoate
2,6-Dimethylaniline Nonanal
2,6-Dimethylphenol 1-Octanol
2-Ethylhexanoic acid Undecane

Column: DB5 30m x 0.25mm x 0.25μm
Temp.: 70°C, 20°C/min to 250°C
Carrier: He at 1.0 mL/min. (constant flow mode)
Inlet: Split (100:1) @ 250°C
Inj: 1μl
Detector: FID, 280°C / H2, 45 mL/min. / Air, 450 mL/min. / He, 40 mL/min.

As you can see the test mix contains a good selection of neutral, acidic and basic compounds with a wide boiling range.  The sample solvent is DCM.

Each of these problems is discussed in the webcast [accessible from the Webcast tab on top of this page] and alongside typical corrective actions and, importantly, preventative actions which can prevent these issues from occurring in future work.


Share this tutorial

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Key Learning Objectives:

  • Recognize key GC issues from the appearance of the chromatogram or baseline
  • Appreciate the requirements for high quality gas supplies and understand how to achieve the optimum in gas cleanliness
  • Understand the optimal way to configure a split / splitless injection system and the routine maintenance and checks to keep it working properly
  • Understanding column loading and designing appropriate analyses to avoid column overload
  • Understand the principles of column installation and conditioning
  • Investigating issues with column and analyte chemistry
  • Recognize issues with settings or gas supply for common GC Detectors

Who Should Attend:

  • Anyone using GC
  • Anyone wishing to improve their GC troubleshooting skills

Tony Taylor Technical Director, Crawford Scientific