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The CHROMacademy Essential Guide Webcast:
Gradient HPLC - 10 Things You Absolutely Need to Know

Thursday 19th June 2014,
8:00am PDT / 11:00am EDT / 16:00 BST / 17:00 CEST

Everyone loves a top ten list, so here is our list of “Top Ten Things You Absolutely Need to Know About Gradient HPLC”.  Whether you are a novice or seasoned gradient HPLC user there is something in here for everyone.

Presented by Dr Dwight Stoll (Assistant Professor of Chemistry at Gustavus Adolphus College)
and Scott Fletcher (Technical Business Development Manager, Crawford Scientific).

  • Spot the difference - isocratic vs. gradient elution
  • High or low pressure pumps for delivering gradients
  • How to optimize essential gradient parameters
  • Find the perfect equilibrium with the correct column re-equilibration time
  • Don’t dwell on what went wrong, calculate your dwell volume
  • It’s a steep learning curve - what effect does gradient steepness have on a separation?
  • How to transfer methods - changing flow rate, column, length, column I.D.
  • How to avoid (or at least improve) drifting baselines
  • Peak capacity - just how many sample components can be separated?
  • Which gradient profile will shape your chromatography?

Who Should Attend:

  • Anyone working with HPLC
  • Anyone developing HPLC methods
  • Anyone with a large number of components in their sample that cannot be separated using isocratic methods
 
Gradient HPLC

 

sponsored by
Thermo

 


Find out more about this Month's Essential Guide Webcast »

 
 

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ThermoGradient HPLC Tutorial

Everyone loves a top ten list, and this month our Essential Guide webcast detailed our list of "Top Ten Things You Absolutely Need to Know About Gradient HPLC".  Whether you are a novice or seasoned gradient HPLC user there is something in here for everyone.

The following CHROMacademy pages will expand on the topics covered within the webcast and serve as a great resource for producing robust gradient HPLC methods whether you are running your first or hundredth gradient HPLC method.

Share this tutorial

HPLC Gradient Elution - Baseline Drift
Baseline drift is particularly problematic in gradient HPLC.  This article explains what causes baseline drift and how it can be avoided.

http://www.chromacademy.com/chromatography-HPLC-Gradient-Elution-Baseline-Drift.html

 
HPLC Gradients – Getting it right!
We may have examples where gradient methods are not sufficiently reproducible or where our equipment struggles to form a gradient at high percentages of acetonitrile or when running 'quick' gradient methods.  Here are some tips and tricks on how to improve the robustness of gradient methods and some quick calculations to check we're getting it right.
http://www.crawfordscientific.com/Chromatography-Technical-Tips-HPLC-Gradients.html
 
What do you really know about Gradient HPLC ?
Test your knowledge of gradient HPLC with the CHROMacademy Gradient HPLC quiz.

http://www.chromacademy.com/chromatography-gradient-HPLC-quiz.html

 
Isocratic or Gradient?
You have been given a C18 column, an unknown sample and been asked to develop a reversed phase HPLC method.  Where do you start?  Do you use isocratic or gradient elution?  Don't panic!  Let us make the decisions easy for you.  Using the steps in this quick guide and the example scouting gradient we will walk you through the decision making process, taking all the stress and worry away for you!

http://www.chromacademy.com/chromatography-isocratic-or-gradient.html

 
Checklists are only for pilots?
How many times have you been in too much of a hurry to get to get a sequence running, set one tiny instrument parameter wrongly and had to spend the whole of the next day repeating the analysis?  Use our HPLC checklist to make sure this doesn't happen again.
http://www.chromacademy.com/chromatography-HPLC-Checklist.html

 
Gradient HPLC - interactive learning from CHROMacademy
Everything you need to know about gradient HPLC.   This elearning module explains why you would use gradient elution, why peak shape is so much more efficient than in isocratic elution, and how different analyte types behave under gradient elution conditions.  From a practical stand point it will guide you through setting up an initial gradient method and how to optimize each of the method parameters.

Gradient Steepness | Optimising Gradient Analyses | Practical Gradient HPLC

 
HPLC Solvent Pumping Systems - interactive learning from CHROMacademy
The HPLC pump is the heart of the chromatographic system.  It is important to understand how the pump works and what to look for if something in the pumping system fails.  With a plethora of interactive content this CHROMacademy elearning module will be invaluable in helping all users understand the inner workings of an HPLC pumping system.  In relation to gradient HPLC, the step by step guide to determining dwell volume is a must read.

Mixing Solvents, Binary Pumps | Mixing Solvents, Quaternary Pumps
Mixing Solvents, Ternary Pumps | Experimental Determination of System Dwell

 
Understanding Gradient HPLC.
A definitive guide to using, optimizing and troubleshooting Gradient HPLC, all supported by Interactive multi-media from CHROMacademy. This webcast considers key topics such as the mechanisms of gradient HPLC versus isocratic elution, developing robust gradient HPLC methods, the dangers of altering column flow or column dimensions without proper gradient scaling, and the essentials of troubleshooting gradient issues.
http://www.chromacademy.com/Understanding_Gradient_HPLC.html
 
 
 
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Everyone loves a top ten list, so here is our list of “Top Ten Things You Absolutely Need to Know About Gradient HPLC”.  Whether you are a novice or seasoned gradient HPLC user there is something in here for everyone.

Presented by Dr Dwight Stoll (Assistant Professor of Chemistry at Gustavus Adolphus College)
and Scott Fletcher (Technical Business Development Manager, Crawford Scientific).

  • Spot the difference - isocratic vs. gradient elution
  • High or low pressure pumps for delivering gradients
  • How to optimize essential gradient parameters
  • Find the perfect equilibrium with the correct column re-equilibration time
  • Don’t dwell on what went wrong, calculate your dwell volume
  • It’s a steep learning curve - what effect does gradient steepness have on a separation?
  • How to transfer methods - changing flow rate, column, length, column I.D.
  • How to avoid (or at least improve) drifting baselines
  • Peak capacity - just how many sample components can be separated?
  • Which gradient profile will shape your chromatography?

Who Should Attend:

  • Anyone working with HPLC
  • Anyone developing HPLC methods
  • Anyone with a large number of components in their sample
    that cannot be separated using isocratic methods

Scott
Fletcher

Technical Manager
Crawford Scientific

John Langley

Dr Dwight Stoll
Assistant Professor of Chemistry at Gustavus Adolphus College