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The CHROMacademy Essential Guide Webcast:
HILIC Method Development
Making HILIC work for you

Thursday 27th April 2017,
8:00am PST/ 11:00am EST/ 4:00pm BST/ 4:00pm CET


This webcast will provide you with the tools you require to understand and design HILIC methods.  

 Understanding the importance of stationary phase choice and how organic solvent, salt content, pH, and column temperature can effect separations will ensure method development is streamlined and the resulting methods produce robust results.

Presented by

Tony Taylor (Technical Director, Crawford Scientific)
Christophe Auberger (Technical Business Development Manager, Crawford Scientific)

Topics Covered

Practical hints and tips for developing and optimizing a HILIC separation

  • HILIC retention mechanisms
  • Mobile phase solvent choice
  • Effects of pH on HILIC separations
  • Effects of buffers and additives on retention and selectivity
  • Stationary phases

Presenter Information »


HILIC Method Development - Tutorial

This month’s webcast focused on hydrophilic interaction liquid chromatography (HILIC). The CHROMacademy tutorial material will expand on the topics covered in the webcast giving you the knowledge to implement and design robust HILIC methods.

Our Quick guides, CHROMacademy course content, and archived webcasts and tutorials will highlight the fundamental theory of HILIC chromatography, detail how to select important method parameters, and provide tips on troubleshooting HILIC separations.

Watch the webcast

eLearning Modules


This module introduces hydrophilic interaction chromatography (HILIC) as a novel mode of chromatography.  The separation mechanisms are illustrated using interactive animations. Chromatographic parameters (such as stationary phase chemistry, eluent composition, and the use of buffers and additives) which impact chromatography are detailed to allow methods to be developed.  Electrostatic repulsion hydrophilic interaction chromatography (ERLIC) is also discussed.

Introduction to HILIC | HILIC Separation Mechanisms | Stationary Phases | Columns | ERLIC


Quick Guides

The Basics of HILIC

Hydrophilic interaction chromatography (HILIC) is applicable to the separation of very polar, highly water soluble analytes which are often poorly retained (if at all) under reversed phase HPLC conditions.  Although reversed phase HPLC stationary phases, such as those denoted ‘aqueous’, have been developed to work with highly aqueous mobile phases in order to promote retention of these very polar species, often they still do not work.

The Basics of HILIC »

How to Choose a Stationary Phase for HILIC

The important factors to consider when selecting column stationary phases for HILIC separations.

How to Choose a Stationary Phase for HILIC »

10 Tips for HILIC Mobile Phase Optimization

How to select organic solvent, buffer, sample diluent and much more.

10 Tips for HILIC Mobile Phase Optimization »

Troubleshooting HILIC

Tips for troubleshooting the most common problems encountered in HILIC.

Troubleshooting HILIC »

Mixed Mode Chromatography

This webcast considers the technique of mixed mode chromatography and its application areas. The mechanisms of mixed-mode chromatography are discussed alongside the parameters which can be used to optimize selectivity and retention. A series of useful illustrative examples are presented with strategies for column selection and screening to allow this technique to quickly become part of the analytical armory.

Mixed Mode Chromatography - Webcast »

Mixed Mode Chromatography - Tutorial »

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Tony Taylor
Technical Director
Crawford Scientific

Tony comes from a pharmaceutical background and has many years’ research and development experience in small molecule analysis and bio-analysis using LC, GC and hyphenated MS techniques.

Tony is actively involved in method development within the analytical services laboratory at Hall Analytical – which supplies contract research in Chromatography and Mass Spectrometry. He continues to research in novel separation technologies, chromatographic method development and structural characterization, especially in the areas of Extractable and Leachable analysis and Bio-Chromatography.

Tony is the Technical Director of the CHROMacademy and has spent the past 17 years as a trainer and developing on-line education materials in analytical chemistry techniques.

Christophe Auberger
Technical Business Development Manager, Crawford Scientific

Christophe comes from a Pharmaceutical background working for both Pharma and Contract research laboratories and has a wealth of experience working with all sorts of regulations (GLP, GCP, ISO17025).

He has a strong background in LC-MS/MS, developing and validating methods for small molecules and peptides in complex matrices.

Christophe has been with the company since 2011, but has accumulated over 10 year’s experience working as a scientist, study director, team leader and business development manager.