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The CHROMacademy Essential Guide Webcast:
How to Select the Optimum HPLC Column

Thursday 9th November 2017,
11:00am EST/ 8:00am PST/ 4:00pm GMT/ 5:00pm CET

 
parker-balston
 

Selecting the correct HPLC column can greatly impact on chromatographic results and analysis speed.  This webcast will allow you to understand how to select a stationary phase based on analyte chemistry, how particle morphology can be used to improve resolution, and how column dimensions can be altered to increase throughput. 

Presented by

Dwight Stoll (Assistant Professor of Chemistry, Gustavus Adolphus College)

and Tony Taylor (Technical Director, Crawford Scientific)

Topics Covered

Understand the impact of HPLC columns on chromatographic separations

  • Available support materials – what are the relative advantages and disadvantages of fully porous and core-shell materials?
  • HPLC stationary phase classifications – what does it mean and how do they differ?
  • The effect of stationary phase chemistry on retention and selectivity
  • The effect of HPLC column dimensions on chromatographic parameters
  • How to choose the optimum HPLC column for your application

Presenter Information »

 
 

How to Select the Optimum HPLC Column

This month’s webcast focused on practical tips for selecting an HPLC column.  The CHROMacademy material provided in this month’s tutorial will give you further insight into how the physical and chemical parameters of HPLC columns can be selected and manipulated to alter and improve chromatography.

Our Quick guides, CHROMacademy course content, and archived webcasts and tutorials will allow you to further advance your knowledge of HPLC column selection as well as provide additional information on essential column know how, such as, column care and maintenance.

Watch the webcast

eLearning Modules


 
Column Chemistry

This module will help you understand the physical and chemical properties of HPLC columns which can be manipulated to provide optimum chromatography. 

Silica as a Packing Material | Reverse Phase Stationary Phases
Working at Low pH | Working at High pH | Other Stationary Phase Types
 

Quick Guides


How to Choose an HPLC Column

A step by step guide to selecting an HPLC column.

How to Choose an HPLC Column »

 

HPLC Column Dimensions

Understand the effects of HPLC column dimensions on chromatographic parameters.

HPLC Column Dimensions »

 

HPLC Column Abuse

A step by step guide to selecting an HPLC column.

HPLC Column Abuse »

 

HPLC Column Maintenance - Prevention is Better Than Cure

Good column maintenance will increase HPLC column lifetime, ensure robust, reproducible chromatography, and ultimately save time and money. 

HPLC Column Maintenance - Prevention is Better Than Cure »

 

Avoiding Problems Associated With HPLC Column Overload

Practical insights into avoiding column overload.

Avoiding Problems Associated With HPLC Column Overload »

 
What HPLC Operators Need to Know: Part II - Everything Needed to Understand Column Selection Mobile Phase Gradients, and Detectors

Column selection probably one of the most important choices in any chromatographic method. We will share our insights on how to select the correct column each time covering particle size, morphology, physicochemical properties as well as stationary phase selection.  We will also detail how to utilize and optimize HPLC gradients and review the various detectors available and when and how they are best employed.

Everything Needed to Understand Column Selection Mobile Phase Gradients, and Detectors »

 
Core Shell Particles - Present and Future

This webcast will highlight the benefits of core-shell particle technology and the reasons behind their surprisingly high efficiency.  Where core-shell particles fit against other efficiency improving measures such as the use of sub 2μm particles with high pressures and also narrow bore HPLC columns will also be discussed. The benefits of using larger and smaller particle size core-shell particles and how particle size and particle morphology might be used to achieve separation requirements will be considered. The interesting topic of core to shell thickness ratio will be investigated which will in turn lead to a discussion of current limitations and future possibilities with core-shell technology.

Core Shell Particles »

 
Understanding HPLC Column Characterization and Selection

A definitive guide to the advanced classification of HPLC stationary phases.  The session will consider the critical characteristics of HPLC supports and bonded phase ligands for reversed phase HPLC and the way in which these materials are classified in terms of the essential performance characteristics by column vendors and independent testing organizations alike.  We will also consider how classification results can be used to select columns for HPLC method development and the relationships between analyte and column characteristics. A must see for everyone using or developing methods for reversed phase HPLC.

Understanding HPLC Column Characterization and Selection »

 
HPLC Troubleshooting - Autosampler, Column, and Detector Issues

Practical troubleshooting and maintenance information associated with HPLC autosamplers, columns and detectors.  This session examines different autosampler designs, injection valve operations and common problems associated with modern HPLC autosamplers.  Sample solvent and injection volume effects on chromatography will also be discussed in detail.  We will consider column hardware design, materials of construction and associated issues – including minimizing dead volume and avoiding / troubleshooting column blockage issues, especially with the popular sub-2 µm column packing materials.  We will also investigate the importance of controlling the temperature of the column during analysis and the potential chromatographic effects of poor temperature control.  We will conclude by reviewing common HPLC detector hardware problems and the chromatographic issues associated with detector hardware and acquisition settings.   We will build a checklist of suggested maintenance operations as well as outlining common diagnostic tests.

HPLC Troubleshooting - Autosampler, Column, and Detector Issues »

 
 
 
 
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Dwight Stoll
Assistant Professor of Chemistry
Gustavus Adolphus College

Dwight Stoll did his undergraduate work at Minnesota State University, Mankato, receiving B.S. degrees in plant biology and biochemistry in 1999 and 2001. Upon graduation in 1999 he took a job in industry as a research technician with ZirChrom Separations, Inc. At ZirChrom he quickly learned about the liquid chromatography market, and in fact became quite interested in the role of separation science in the development of new analytical methodologies for use in other disciplines such as biology. In 2000 he shored up his chemistry background at the University of Minnesota before enrolling in the graduate program in chemistry there in 2001. At the UofM he studied with Professor Peter Carr, and worked on the development of Fast, Comprehensive Two-Dimensional Liquid Chromatography, using the principles of high temperature and ultra-fast gradient elution liquid chromatography to improve the overall speed of two-dimensional separations. Before receiving the Ph.D. in analytical chemistry in 2007, he took a nine-month break from graduate studies to teach as an adjunct faculty member at St. Olaf College where he taught analytical and general chemistry. Following graduate in 2007, he spent nine months working as a post-doctoral fellow with Dr. Christine Wendt in the Lung Health Center at the University of Minnesota, where he began analyzing the low molecular weight constituents of human lung lavage fluid using liquid chromatography coupled with mass spectrometry. In the fall of 2008, Dwight began his faculty career as assistant professor in the chemistry department at Gustavus Adolphus College, where he mainly teaches quantitative and instrumental analysis courses, in addition to directing a vibrant research program involving mainly undergraduate students. His active research projects include the development of rapid multidimensional liquid chromatography for both targeted and untargeted analysis in complex matrices, optimization of isocratic and gradient elution HPLC, and the characterization and role of selectivity in reversed-phase HPLC.
Dwight is the author or co-author of 35 peer-reviewed publications and two book chapters in the area of separation science. He has authored or co-authored over 80 presentations at local, national, and international meetings. In 2009 he was the winner of the John B. Phillips Award for contributions to multidimensional gas chromatography, and in 2011 he was the recipient of LCGC’s Emerging Leader in Chromatography Award. Most recently he was recently elected to the editorial advisory boards of LCGC Magazine, the leading trade publication for the separation science community, and the separation science journal Chromatographia.


Tony Taylor
Technical Director, Crawford Scientific

Tony comes from a pharmaceutical background and has many years’ research and development experience in small molecule analysis and bio-analysis using LC, GC and hyphenated MS techniques.
Tony is actively involved in method development within the analytical services laboratory at Hall Analytical – which supplies contract research in Chromatography and Mass Spectrometry. He continues to research in novel separation technologies, chromatographic method development and structural characterisation, especially in the areas of Extractable and Leachable analysis and Bio-Chromatography.
Tony is the Technical Director of CHROMacademy and has spent the past 17 years as a trainer and developing on-line education materials in analytical chemistry techniques