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HPLC Method Development - Video Training Course
Session 3

 
 
Eluent Optimization and Gradient Development I

Following our logical development pathway, in session 3 we use computer modelling software to highlight key approaches to optimising eluotropic strength and pH when dealing with ionogenic compounds as well as buffer selection and buffer strength.

We also consider the fundamental separation mechanisms at play in gradient HPLC and begin our discussions on developing and optimizing gradient HPLC parameters.

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session 1 details session 2 details session 3 details session 4 details

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Tutor support

Amy Claydon BSc PhD

Amy began using chromatography at the start of her academic career with nanoflow-liquid chromatography-mass spectrometry, and a variety of other proteomics techniques, for her research into the dynamic proteome. The laboratory also focused on gas chromatographic separations of volatile biological samples.
After using chromatography for 10 years, Amy has now moved into a more training-based role with Crawford Scientific, where she hopes to share the knowledge she gained during her time in the laboratory.

  Amy Claydon

Tony Taylor B.Sc. CChem FRSC Cert. Ed.

Tony has been doing, researching, teaching and training in analytical chemistry for the past 28 years. He comes from a pharmaceutical and polymer analysis background and continues to work with both liquid and gas phase techniques at Crawford Scientific (UK).
His main interests are the use of LC-MS and GC-MS for structural characterisation and the quantitation of trace components in complex matrices. He is professionally qualified trainer and is Technical Director of the CHROMacademy.

  Tony Taylor

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FAQs will be added as students progress through the course.

 
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