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The CHROMacademy Essential Guide Webcast: HPLC Techniques in Biopharmaceutical Analysis
Thursday 21st August 2014,

Biopharmaceuticals offer great hope in treating medical conditions which are currently poorly served at best by traditional pharmaceuticals.  It is estimated that there are over 400 biopharmaceuticals in clinical trails for in excess of 200 disease areas.  The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing.  This Essential Guide webcast will provide details of why differing modes of chromatography are required in the research, preparation and analysis of biopharmaceuticals and when they would be typically used.

  • Introduction to Biopharmaceuticals
  • Protein characteristics of analytical interest
  • Use of various HPLC techniques to analyze proteins at Intact (Native), Fragmented, Peptide, Glycan and Amino acid levels including;
  • Reversed Phase (RPLC)
  • Size Exclusion Chromatography (SEC)
  • Ion Exchange Chromatography (IEX)
  • Hydrophilic Interaction Chromatography (HILIC)
  • Affinity Chromatography
  • Advances in throughput, specificity and accuracy for peptide mapping, charged variant analysis, aggregation and titer analysis

Key Learning Objectives:

  • Understand the differences between traditional small molecule and larger biopharmaceuticals and their relative advantages
  • Review the complexity associated with producing and analyzing biopharmaceutical products
  • Identify the commonly employed LC techniques when working with biopharmaceuticals
  • Learn how and when each of the techniques are employed and associated benefits

Who Should Attend:

  • Anyone interested in analyzing biopharmaceuticals
  • Anyone working in analysis peptides, proteins or monoclonal antibodies
  • Anyone working with HPLC
  • Anyone who wishes to learn about different HPLC techniques


Find out more about this Month's Essential Guide Webcast »

 
 


HPLC Techniques in Biopharmaceutical Analysis

Biopharmaceuticals offer great hope in treating medical conditions which are currently poorly served, at best, by traditional pharmaceuticals. It is estimated that there are over 400 biopharmaceuticals in clinical trials for over 200 disease areas. The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, means that chromatography is employed to a much greater extent during production and release testing.

This Essential Guide webcast covers details of why differing modes of chromatography are required in the research,
preparation, and analysis of biopharmaceuticals and when they would be typically used.

Furthermore, mass spectrometry is commonly employed in the analysis of biomolecules and biopharmaceuticals;
therefore, we have highlighted some of the most relevant information in CHROMacademy to further your knowledge
and understanding of the analysis of biopharmaceuticals.

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Fundamentals of MS Proteomics Research
This CHROMacademy elearning module will introduce the basic principles of proteomic mass spectrometric analysis of simple biomolecules. The soft ionization techniques employed for proteomics research are presented in order to provide a good basic understanding of the mass spectrometric instrumentation and process. Finally, the interpretation of mass spectra will also be covered to provide the complete basic introduction to proteomics research.

MS Structure Determination | ESI Considerations | MALDI Ionisation Techniques in Proteomics

Multiply Charged Peptides | Multiply Charged Ions | Peptide and Protein Digestion

 
Characterization of Protein Biopharmaceuticals
Protein biopharmaceuticals such as monoclonal antibodies and recombinant proteins are currently in widespread use for treatment of various life-threatening diseases including cancer, autoimmune diseases, diabetes, anemia, etc. Protein therapeutics have a complexity far exceeding that of small molecule drugs, hence, unraveling this complexity represents an analytical challenge. This web seminar will highlight the power of liquid chromatography combined with mass spectrometry in the characterization of protein biopharmaceuticals.

Watch this webcast »

 
The Analysis of Post-Translational Modifications Using LC-MS/MS
Dr. Duncan Smith and Dr. John Griffiths (MS-Insight Ltd and Manchester University) explain the principles behind the analysis of a number of key post-translational modifications (PTMs) using LC-MS/MS techniques. They will begin with a brief overview of bottom-up proteomics, explaining how proteins are converted to peptides, which are then analyzed using electrospray LC-MS/MS. They will move on to talk about the various MS scanning techniques, which may be used for PTM analysis. Next, they will discuss in turn, how phosphorylation, acetylation, ubiquitination, and SUMOylation may be analyzed using mass spectrometry, drawing upon examples from their own research at the University of Manchester. Finally, they will give their own opinions on the ways in which they see the future of PTM analysis heading.

Watch this webcast »

 
 
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Key Learning Objectives:

  • Understand the differences between traditional small molecule and larger biopharmaceuticals and their relative advantages
  • Review the complexity associated with producing and analyzing biopharmaceutical products
  • Identify the commonly employed LC techniques when working with biopharmaceuticals
  • Learn how and when each of the techniques are employed and associated benefits

Who Should Attend:

  • Anyone interested in analyzing biopharmaceuticals
  • Anyone working in analysis peptides, proteins or monoclonal antibodies
  • Anyone working with HPLC
  • Anyone who wishes to learn about different HPLC techniques

Davy Guillarme PhD

Senior lecturer
School of Pharmaceutical Sciences
University of Geneva, University of Lausanne

Scott Fletcher

Business Development Manager
Crawford Scientific