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The CHROMacademy Essential Guide Webcast:
Troubleshooting LC-MS

Tuesday 28th February 2017,
8:00am PST/ 11:00am EST/ 4:00pm GMT/ 5:00pm CET




This webcast will present a logical approach to identifying whether a problem has been caused by the sample/method or the instrumentation itself, followed by identifying which instrument component may be at the root of the issue. We will share tips and trick to help you identify the most common problems and how to properly set up your method and instrument.

Topics Covered

  • Tools to diagnose the source of a problem
  • How to tune and what the tune report tells us
  • Troubleshooting common LC-MS issues
    • Contamination
    • Ion suppression or enhancement
    • Misidentification of the molecular ion
    • Poor signal reproducibility
  • Method/system parameters which should be considered
    • Buffer, flow rate, sample concentration
    • Use of divert valves, using scan function to visualize co-elution problems, source temperature


Presenter Information »


Troubleshooting LC-MS - Tutorial

This month’s webcast focused on troubleshooting LC-MS.  The CHROMacademy material provided in this month’s tutorial will provide you with even more knowledge on troubleshooting both HPLC and MS components.

Our Quick guides, CHROMacademy course content, and archived webcasts and tutorials will highlight which instrument and method parameters are important to understand in order to produce good quality LC-MS data and keep your instruments functioning well.

Watch the webcast

Troubleshooting Videos

Practical HPLC Troubleshooting Video HPLC Troubleshooting Video Guide to High Pump Backpressure

High back-pressure in the system results in the pump working under greater resistance which will lead to an increase in the need for maintenance. Learn how to locate the source of high backpressure in your HPLC system and resolve the problem.

High Pump Backpressure »

Practical HPLC Troubleshooting Video Practical HPLC Troubleshooting Video - Retention Time and Selectivity

This example solves an intractable problem of a fast HPLC-MS separation which shows small but disruptive changes in retention time and selectivity for a number of ionizable compounds. The CHROMacademy troubleshooter is used to diagnose the problem(s) after which we explain how to avoid problems with robustness when using eluent additives which are compatible with mass spectrometric detection.

Retention Time and Selectivity »

Practical HPLC Troubleshooting Video Practical HPLC Troubleshooting Video - Cycling Baseline

In this video, we solve a cycling HPLC baseline problem submitted to our Ask the Expert service by a CHROMacademy member.  This video shows how our interactive HPLC Troubleshooter can be used to identify potential causes for the problem.  The potential causes, how they might affect the appearance of our baseline, and what to do to avoid the problems happening again are discussed.

Cycling Baseline »

Practical GC Troubleshooting Video Practical HPLC Troubleshooting - Split Peaks

Split peaks in HPLC are one of the most common problems we get asked about at CHROMacademy.  With numerous causes related to both the instrument and method it can be a potentially complex problem.  Watch this troubleshooting video to see how the CHROMacademy HPLC troubleshooter can help to quickly solve this problem … and pick up a few extra troubleshooting tips too.

Split Peaks »

Quick Guides

Avoiding LC-MS Signal Suppression

Some practical insights into optimizing LC-MS eluents and parameters to avoid signal suppression.

Avoiding LC-MS Signal Suppression »

My LC-MS isn’t behaving! Where do I start?

A step-by-step guide to using a simple benchmarking method for troubleshooting LC-MS problems.

My LC-MS isn’t behaving! Where do I start? »

10 Ways to Break Your LC-MS

Do you do any of these 10 things?

10 Ways to Break Your LC-MS »

Chromatography Troubleshooting Tips

Essential troubleshooting tips for all chromatographers.

Chromatography Troubleshooting Tips »

What LC-MS Operators Need to Know

The definitive guide to what every LC-MS operator needs to know about this powerful technique in order to get the most out of every analysis.

What LC-MS Operators Need to Know »

HPLC Troubleshooting Masterclass

Practical HPLC separation troubleshooting using real world chromatograms submitted by CHROMacademy members from many different application areas, they illustrate how to recognize problems and explain further tests and investigations necessary to properly qualify the issue.  Advice is given on how the problem may be resolved to help you gain valuable insight into recognizing, characterizing, and fixing problems in HPLC to improve your data quality and reduce instrument downtime.

HPLC Troubleshooting Masterclass »

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Prof. Kevin Schug (Professor and the Shimadzu Distinguished Professor of Analytical Chemistry, Department of Chemistry and Biochemistry, The University of Texas at Arlington)

Kevin A. Schug is Professor and the Shimadzu Distinguished Professor of Analytical Chemistry in the Department of Chemistry and Biochemistry at The University of Texas at Arlington (UTA). 

He received his B.S. degree in Chemistry in 1998 from the College of William and Mary, and his Ph.D. degree in Chemistry from Virginia Tech in 2002 under the supervision of Prof. Harold M. McNair. 

From 2003-2005, he performed post-doctoral research in the laboratory of Prof. Dr. Wolfgang Lindner at the University of Vienna in Austria. Since joining UTA in 2005, his research has been focused on the theory and application of separation science and mass spectrometry for solving a variety of analytical and physical chemistry problems.  He has over 100 peer-reviewed publications and 400 presentations, posters, and invited talks to his group’s credit. 

He has been the primary mentor and research advisor to more than 20 graduate and 50 undergraduate students.  Dr. Schug has received the 2009 Emerging Leader in Chromatography award given by LCGC magazine, an NSF CAREER award, the 2009 Eli Lilly and Company ACACC Young Investigator Award in Analytical Chemistry, and the 2013 American Chemical Society Division of Analytical Chemistry Young Investigator in Separation Science Award.

For his teaching, he received the 2014 University of Texas System Regents’ Outstanding Teaching Award and was named in 2016 as a Fellow of the University of Texas System Academy of Distinguished Teachers. He is a member of the Editorial Advisory Board of LCGC Magazine (Advanstar), Analytica Chimica Acta (Elsevier), and the Journal of the American Society for Mass Spectrometry (Springer). He is a Senior Editor for Journal of Separation Science (Wiley).

Dr. Dawn Watson, CHROMacademy Technical Expert,
Crawford Scientific

Dawn received her PhD in synthetic inorganic chemistry from the University of Strathclyde, Glasgow.  The focus of her PhD thesis was the synthesis and application of soft scorpionate ligands.  As well as synthetic skills, this work relied on the use of a wide variety of analytical techniques, such as, NMR, mass spectrometry (MS), Raman spectroscopy, infrared spectroscopy (IR), UV-visible spectroscopy, electrochemistry, and thermogravimetric analysis. 

Following her PhD she spent two years as a postdoctoral research fellow at Princeton University studying the reaction kinetics of small molecule oxidation by catalysts based on Cytochrome P450.  In order to monitor these reactions stopped-flow kinetics, NMR, HPLC, GC-MS, and LC-MS techniques were utilized.  Prior to joining the Crawford Scientific and CHROMacademy technical team she worked for Gilson providing sales and support for the entire product range including, HPLC (both analytical and preparative), solid phase extraction, automated liquid handling, mass spec, pipettes, and laboratory consumables.