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The CHROMacademy Essential Guide Webcast:
What LCMS Operators Need to Know
 

Thursday 19th November 2015,
8:00am PDT / 11:00am EDT / 16:00 BST / 17:00 CEST

Many people use HPLC with Mass Spectrometric Detection without properly understanding the working principles or key parameters.  In this webcast you will learn;

  • The working principles of API-MS interfaces
  • How to optimize mobile phase composition and flow for best response
  • How to design and optimize your mass analyzer settings
  • How to spot and fix problems

If you are using HPLC-MS instruments, and would like to know more about how they work and how to get the best from them, this webcast is for you!

Presented by Tony Taylor (Technical Director, Crawford Scientific, UK).



Key Learning Objectives:

  • Understand the key working principles of ESI and APCI ionization mechanisms
  • Understand the key variables in LCMS methods and how they can be optimized
  • Understand how to optimize mobile phase composition for best separation and detector response
  • Ability to choose appropriate mass analyzer settings for good MS and MS/MS response for a range of analyzer types

Who Should Attend:

  • Anyone who wants to improve their understanding and of LCMS techniques
  • Anyone wishing to improve the quality of their LCMS data
 
 
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Find out more about this Month's Essential Guide Webcast »

 
 

What LCMS Operators Need to Know

Every LCMS operator should have certain knowledge and skills in order to produce the right quality data in the most efficient manner.  This webcast highlights this knowledge and provides a very pragmatic guide to the day to day operation of an HPLC system with Mass Spectrometric detection as well as a valuable insight into the important variables from any LC-MS method.

Every MS detector needs to be set-up properly prior to analysis, which may include checks on important API interface parameters such as gas flows, nebulising voltages, source temperature and MS acquisition settings etc.  A checklist of these items is good to follow and is often included in an instrument operation SOP – but do we always follow the SOP?  When was the last time you questioned WHY, these variables are set at the values chosen?  Have you ever used the phrase – ‘oh that value works for all of our methods’?  If so then this webcast and accompanying tutorial is for you!

Once the method is set and equilibrated, test injections need to be made to assess the suitability of the system to produce valid analytical data.  The baseline, chromatographic appearance and any quantitative output should be evaluated for suitability.  What is the best way to do this?

Every LC-MS operator should be able to critically evaluate the method being implemented and understand the instrument settings as well as being able to notice parameter settings which are out of the ordinary.  Problems may be anticipated and any allowable adjustments made to ensure that satisfactory data is produced.

Only by having the ability to properly implement an analysis and evaluate the quality of the data produced can an LCMS operator produce data which is fit for purpose on an on-going basis.  Are YOU fit for purpose?  This webcast will help to ensure that you are!

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eLearning Modules


Electrospray Ionization Theory Electrospray Ionization Theory

In order to properly understand the effects of variables on instrument response, LC-MS operators need to understand the effects that changing these variables can have. This module contains key information on the major variables which contribute to instrument sensitivity and reproducibility when using Electrospray ionization, including Nebulizing Voltage and Nebulizing Gas Flow.

Production of charged droplets at the capillary tip | Droplet desolvation and jet fission
Electrospray production – Eluent flow rate | Ion suppression principles

 
Mass Analyzers Mass Analyzers

Collecting the correct spectral information is a primary concern for the LC-MS operator. There are many parameters associated with MS detector acquisition that are typically not specified in methods and the user should be aware these ‘lock and leave’ parameters to avoid issues with the data obtained.

Quadrupole voltages | Mass Gain and Offset | Quadrupole Ion Trajectories

 
 
Quick Guides

Electrospray Ionization Theory 10 Must Know Points to Understand Electrospray Ionization
  1. Eluent pH adjusted to obtain ionised forms of the analyte in solution – retention in reversed phase HPLC may need to be considered and an appropriate stationary phase chosen.  The eluent flow rate is adjusted so as not to ‘swamp’ the source – typically <1 mL/min
  2. A voltage is applied between the capillary and sampling plate to generate an Electrospray  - a special circuit which is completed by the movement of analyte ions through space (the desolvation zone) ...

10 Must Know Points to Understand Electrospray Ionization »

 
Atmospheric Pressure Chemical Ionization 7 Must Know Points to Understand Atmospheric Pressure Chemical Ionization
  1. Atmospheric Pressure Chemical Ionization (APCI) uses gas-phase ion-molecule reactions at atmospheric pressure. It relies on the formation of a plasma of ions comprised, mainly, from the HPLC mobile phase components.
  2. In APCI the eluent is introduced into the interface using a capillary of similar design to the ESI source.  However, no potential is applied to the capillary, instead the liquid emerges from the capillary surrounded by a flow of inert nebulizing gas into a heated, vaporizing region...

Atmospheric Pressure Chemical Ionization »

 
The CHROMacademy Essential Guide to Electrospray Ionization (ESI) for LC-MS

Learn how to optimize the essential parameters and improve instrument response and reproducibility in your lab. Improve your electrospray data and troubleshoot your ionization and data interpretation problems more effectively. Learn the fundamental basis of ion suppression so you can take best steps to avoid this phenomenon and discover best practice for acquiring qualitative data. Find out this and much more here…

Electrospray Ionization (ESI) for LC-MS »

 
Introduction to Quadrupole Mass Anayzers and MS/MS Analysis

Quadrupole mass analyzers are the most common type of mass analyzers found in analytical laboratories. They were originally thought to be only suitable for residual gas analysis due to the limited mass range over which they operate, however with the advent of Electrospray Ionization (ESI) they can now be used for analytes whose masses exceed tens of thousands of Daltons, due to multiple charging effects.

Quadrupole Mass Anayzers and MS/MS Analysis »

 
LCMS Method Parameters - Critical Evaluation

In this session Arindam Roy (Associate Director, Quality, Novartis Consumer Health) and Scott Fletcher (Technical Manager, Crawford Scientific) will consider a range critical LC-MS method parameters and their impact on analytical sensitivity and specificity. The importance and impact of specific eluent chemistry, interface parameters, analyzer settings and types are all critically reviewed and evaluated in this highly relevant presentation.

LCMS Method Parameters - Critical Evaluation »

 
 
 
 
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Key Learning Objectives:

  • Understand the key working principles of ESI and APCI ionization mechanisms
  • Understand the key variables in LCMS methods and how they can be optimized
  • Understand how to optimize mobile phase composition for best separation and detector response
  • Ability to choose appropriate mass analyzer settings for good MS and MS/MS response for a range of analyzer types

Who Should Attend:

  • Anyone who wants to improve their understanding and of LCMS techniques
  • Anyone wishing to improve the quality of their LCMS data

Tony Taylor
Technical Director
Crawford Scientific