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The CHROMacademy Essential Guide Webcast:
Making the Right Choices in SEC Method Development

Thursday 16th March 2017,
8:00am PST/ 11:00am EST/ 3:00pm GMT/ 4:00pm CET

 

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This webcast will focus on the parameters which are essential for the development of robust size exclusion methods; selecting the correct column pore size for your molecules, how to design optimum mobile phases for resolution, and the manipulation of parameters such as column dimensions and flow rate to allow for faster analyses.

Presented by

Dr. Andrew Coffey (Senior Applications Chemist, Agilent Technologies).

Topics Covered

  • Selecting the right pore size
  • Mobile phase consideration
    • Buffer selection
    • Using buffer advisor software
    • Salt concentration
  • Effect of column dimensions

Presenter Information »

 
 

Making the Right Choices in SEC Method Development - Tutorial

This month’s webcast focused on method development for the analysis of biopharmaceuticals using size exclusion chromatography.  The CHROMacademy material provided in this month’s tutorial will help you understand all of the parameters which must be considered when developing an SEC method; from column dimensions, to mobile phase selection, and detection methods.

Our Quick guides, CHROMacademy course content, and archived webcasts and tutorials provide practical information on designing an SEC methods and what information can be gained from these types of analyses.

Watch the webcast

eLearning Modules


 
Size Exclusion Techniques

Learn how size exclusion chromatography is used to analyze biomolecules and what information can be gained. Stationary phase, mobile phase, and instrument considerations will be discussed in relation to employing successful methods.

Basics of SEC | SEC Calibration Curves | SEC for Biomolecules

 
mAbs - Introduction to Monoclonal Antibodies

An introduction to the monoclonal antibodies, including structural features and post-translational modifications.

Introduction to Monoclonal Antibodies »

 

Quick Guides


HILIC, IEX, and SEC for the Analysis of Biomolecules

The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing. This article will examine the use of HILIC, IEX, and SEC for their full characterization.

HILIC, IEX, and SEC for the Analysis of Biomolecules »

 
Selecting Columns and Initial Conditions for IEX and SEC

Biopharmaceutical compounds are genetically engineered from living cells and have molecular weights in the range 2,000-2,000,000 Da with 10-2,000 reactive groups and are often comprised of a mixture of closely related variants; therefore, there is a wider degree of variability (a degree of heterogeneity) in comparison with small molecule pharmaceuticals.

Selecting Columns and Initial Conditions for IEX and SEC »

 
Biopharmaceuticals Quiz

A little bit of fun to test your knowledge of biopharmaceuticals and their analysis.

Biopharmaceuticals Quiz »

 
Characterization of Protein Biopharmaceuticals

Protein biopharmaceuticals, such as monoclonal antibodies and recombinant proteins, are currently in widespread use for treatment of various life-threatening diseases including cancer, autoimmune diseases, diabetes, anemia, etc. Protein therapeutics have a complexity far exceeding that of small molecule drugs, hence, unraveling this complexity represents an analytical challenge. This webcast will highlight the power of liquid chromatography combined with mass spectrometry in the characterization of protein biopharmaceuticals.

Characterization of Protein Biopharmaceuticals »

 
HPLC Techniques in Biopharmaceutical Analysis

The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing. This Essential Guide webcast will provide details of why differing modes of chromatography are required in the research, preparation, and analysis of biopharmaceuticals and when they would be typically used.

HPLC Techniques in Biopharmaceutical Analysis »

 
Techniques Employed in Biopharmaceutical Analysis - Part II Ion Exchange and SEC

The definitive guide to optimizing methods for GCMS analysis. In this session we consider the major instrument and method decisions that allow you to get the very best from your analysis.

Ion Exchange and SEC »

 
 
 
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Dr. Andrew Coffey
Senior Applications Chemist
Agilent Technologies

Andy joined Polymer Laboratories in 1989 after completing his PhD in peptide synthesis at Wolverhampton, UK. He progressed from Senior Scientist to Product Manager looking after the HPLC column product line before focusing on the Solid Phase specialty particle businesses.

He continued as Product Manager adding Biosolutions and Flash chromatography consumables product lines to his portfolio during the acquisition by Varian and then Agilent Technologies, and joined the Biocolumns Applications Team in August 2011.

Andy has considerable experience in reversed phase, ion-exchange and size exclusion liquid chromatography method development, technical support, and troubleshooting - and has a “hands on” attitude to problem solving!