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Practical HPLC Troubleshooting Video
 

Solving retention time and selectivity changes in LCMS method transfer - Live HPLC Troubleshooting from CHROMacademy

This examples solves an intractable problem of a Fast HPLC-MS separation which shows small but disruptive changes in retention time and selectivity for a number of ionisable compounds. The CHROMacademy troubleshooter is used to diagnose the problem(s) after which Tony Taylor explains how to avoid problems with robustness when using eluent additives which are compatible with Mass Spectrometric detection.

 

 

 

 

HPLC troubleshooting
 
 
 

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