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Practical HPLC and GC Troubleshooting Videos Archive
GC Troubleshooting Video Guide to irreproducible retention times

Retention time shifts are not uncommon in GC, and the magnitude of the shift can be both highly significant and problematic.  A quick troubleshooting video guide to which instrument and method parameters can cause short or long term irreproducibility, how to fix the problem, and avoid it occurring in the future.

  Practical GC Troubleshooting Video

HPLC troubleshooting video guide to high pump backpressure

High back-pressure in the system results in the pump working under greater resistance which will lead to an increase in the need for maintenance.  Learn how to locate the source of high backpressure in your HPLC system and resolve the problem.

  Practical HPLC Troubleshooting Video

Practical GC Troubleshooting Video - Peak Area Reproducibility

Learn how to solve the problem of irreproducible peak areas, and how to avoid this issue in the future, with this troubleshooting video.  Irreproducible peak areas may not be immediately noticed until your data is analyzed as the peaks themselves may exhibit good shape and consistent retention times.  Variable peak areas can have numerous causes and may indicate a problem with the GC system itself.
  Practical GC Troubleshooting Video

GC Troubleshooting Video Guide to Split, Broad, and Fronting Peaks

This video guide looks at solving the problem of split, broad, and fronting peaks in a real chromatogram using the CHROMacademy GC Troubleshooter.  Split peaks are a very common problem; therefore, additional troubleshooting tips will be given which can be used if you ever see split peaks in your chromatogram.
  Practical GC Troubleshooting Video

Practical HPLC Troubleshooting Video - Retention time and Selectivity

This examples solves an intractable problem of a Fast HPLC-MS separation which shows small but disruptive changes in retention time and selectivity for a number of ionisable compounds. The CHROMacademy troubleshooter is used to diagnose the problem(s) after which Tony Taylor explains how to avoid problems with robustness when using eluent additives which are compatible with Mass Spectrometric detection.
  Practical HPLC Troubleshooting Video

Practical GC Troubleshooting Video - Peak Splitting

In this video, we solve a problem with one split peak, in the middle of a GC chromatogram submitted to our Ask the Expert service by a CHROMacademy member. This video shows Tony Taylor (Technical Director, Crawford Scientific) explaining how to identify peak shape problems which occur in the middle of the elution range - often the most difficult problems to solve. He then discusses several potential causes, how to logically identify the source of the problem and what to do to avoid these issues happening again! Tune in to see what eventually fixed the problem!
  Practical GC Troubleshooting Video

Practical HPLC Troubleshooting Video - Cycling Baseline

In this video, we solve a cycling HPLC baseline problem submitted to our Ask the Expert service by a CHROMacademy member.  This video shows Tony Taylor (Technical Director, Crawford Scientific) explaining how to use the interactive HPLC Troubleshooter to identify potential causes for the problem.  He then discusses each of the potential causes, how they might affect the appearance of our baseline and what to do to avoid the problems happening again.
  Practical HPLC Troubleshooting Video

Practical GC Troubleshooting Video - Peak tailing

In this video, we solve a GC peak tailing problem submitted to our Ask the Expert service by a CHROMacademy member.  This video shows Tony Taylor (Technical Director, Crawford Scientific) explaining how to use the interactive GC Troubleshooter to identify potential causes for the problem.  He then discusses each of the potential causes, how they might affect our chromatography and what to do to avoid them happening again.
  Practical GC Troubleshooting Video

 
 
 

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