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CHROMacademy essential guides

GC-MS/MSTranslating GC and GCMS Methods »

In this session we cover the various reasons for translating methods including improved chromatography, faster throughput, adopting modern column geometries and switching to hydrogen carrier from helium. We explore all aspects of instrument hardware and method variable translation and you will leave with a ‘toolkit’ approach for translating your own methods – saving you time, money and improving your chromatography!

Topics covered include:

  • Key reasons and benefits of translating GC and GCMS methods
  • Translating for speed, efficiency or to hydrogen from helium
  • Maintaining retention time
  • Avoiding selectivity changes in temperature programmed GC
  • Effects of changing carrier on detector performance & sensitivity
  • MS detector considerations
 

 

HPLC Troubleshooting Masterclass »

HPLC Troubleshooting Masterclass »This month we will use your chromatograms to help illustrate the optimum approach to troubleshooting HPLC problems. Using real world chromatograms submitted by CHROMacademy members from many different application areas, we illustrate how to recognize problems and explain further tests and investigations necessary to properly qualify the issue. 

Topics covered include:

  • Learn how experts troubleshoot HPLC chromatography problems
  • A series of real chromatograms are used to demonstrate logical ways to approach various issues in HPLC
  • Learn what problems and symptoms are important and how to further investigate issues
  • Learn what further tests can be done to better understand problems and fix issues more quickly
  • Learn to recognize problems quickly and achieve better data quality and higher throughput
 

Choosing the Correct Sample Preparation Technique l Live Webcast & Tutorial

Thursday 22th May 2014

Choosing the Correct Sample Preparation Technique “Before anything else, preparation is the key to success” Alexander Graham Bell. This is certainly true in analytical chemistry. The correct choice of sample preparation method for a sample prior to analysis can have a myriad of benefits; properly prepared samples will produce simpler chromatograms free from interfering peaks, instrument parts will be protected from harmful contaminants which could result in break downs and the need for increased maintenance, and the sample will be compatible with the analysis method (correct pH, solvent etc.) minimizing deleterious chromatographic effects.

Topics covered include:

  • What sample preparation techniques are available
  • What are the underlying chemical mechanisms which lead to selectivity within sample preparation
  • Which sample preparation techniques are applicable to particular sample types and applications
  • What are the relative merits and drawbacks of each commonly employed technique
  • How to choose the correct sample preparation technique

Top 10 Features of a successful Chromatography Data System - Part 2 l Webcast

Tuesday 27th May 2014

Chromatography Data System In this second session , Tony Taylor (Technical Director, Crawford Scientific Ltd.) and Barbara van Cann (Product Specialist Chromatography Software, Thermo Scientific) explore the important aspects of a Chromatography Data System and highlight specific examples using the Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS) software. We further explore questions posed by chromatographers regarding Chromatography Data Systems including:  flexible quantitation and reporting tools, regulatory compliance and ease of ownership issues.

 

Topics covered include:

  • Continuing from our highly successful first event
  • Posing key questions that chromatographers need to have answered
  • Investigating key features such as:
  • Built-in calculation and reporting options
  • Ensuring 24-7 uptime
  • Compliance with regulations
  • Cost of ownership
  • Ease of administration
  • Support

GC-MS Ionization Processes l Webcast & Tutorial

Thursday 24th April 2014

GC-MS-IonizationThe focus of this month’s Essential Guide webcast is GC-MS ionization processes. The two main forms of ionization, Electron Ionization (EI) and Chemical Ionization (CI), will be discussed in depth. The molecular reactions which occur within the ion source to produce ions will be examined along with which form of ionization should be used for particular analytes. Finally, our speakers Dr. John Langley (Southampton University) and Tony Taylor (Crawford Scientific) will impart some of their expert knowledge regarding optimization of the GC-MS process and some general interpretation strategies.


Topics covered include:

  • Why use GC-MS
  • Anatomy of a typical ion source
  • Electron and Chemical Ionization – explanation of key parameters and how they effect your analysis
  • How to improve sensitivity and specificity by manually tuning source parameters
  • Recognizing ionization problems in your chromatographic and mass spectral data
  • Analyte fragmentation and basic interpretation strategies

Critical Choices in HPLC – Selecting Column Stationary Phase and Dimensions l Webcast & Tutorial

Thursday 20th March 2014

GC-MS-IonizationHow do you currently select a column for an HPLC application? Do you just use the one that is on the instrument, root around in an old drawer and hope that you pull out one that works, use your favorite C18 or ODS column, or borrow one from a colleague who’s chromatography is currently working particularly well? These are probably not ideal situations but there will be many of us who are guilty of these exact scenarios. Instead, why not join us for the CHROMacademy Essential Guide webcast on HPLC column selection in which we will look at the available column stationary phases and support materials (including conventional fully porous and core-shell/superficially porous material), column dimensions and the effect that they can have on your chromatography, and how to optimize and improve your current chromatographic separations. This practical guide will give you the tools required to make an informed choice every time you develop a new HPLC method.


Topics covered include:

  • Available support materials
    what are the relative advantages and disadvantages of fully porous and core-shell materials?
  • HPLC stationary phase classifications – what does it mean and how do they differ?
  • The effect of stationary phase chemistry on retention and selectivity
  • The effect of HPLC column dimensions on chromatographic parameters
  • How to choose the optimum HPLC column for your application

Top 10 Features of a successful Chromatography Data System l  Webcast

Tuesday 11th March 2014

Chromatography Data SystemThe Essential Guide from LCGC’s CHROMacademy presents the definitive guide to the Top 10 features of a successful Chromatography Data System (CDS).  In this session, Tony Taylor (Technical Director, Crawford Scientific Ltd.) and Barbara van Cann (Product Specialist Chromatography Software, Thermo Scientific) explore the important aspects of a Chromatography Data System and highlight specific examples using the Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data System (CDS) software. We explore all aspects including compliance, good practice in Instrument Control, effective data processing, ease of use, user defined quantitation models and reporting.  A must watch for anyone who would like to use their own data system more effectively or are seeking a more effective CDS platform.

  • Even if you aren’t looking for a new CDS – this webcast will be educational!
  • What key features should you look for in a CDS system?
  • What the best systems can and should offer you in terms of:
  • Ease of use
  • Instrument control and data acquisition
  • Integration, Calibration and Data Processing
  • Flexible reporting
  • Regulatory compliance
  • Scalability

Translating to Hydrogen Carrier for GC – A Practical Perspective l Webcast

Tuesday 18th February 2014

Translating to Hydrogen Carrier for GCThe Essential Guide from LCGC’s CHROMacademy presents the definitive guide in translating your GC and GCMS methods from Helium to Hydrogen carrier gas.  In this session, Tony Taylor (Technical Director, Crawford Scientific) and Dr. Dawn Watson (Technical Expert, CHROMacademy, Crawford Scientific) explore the various reasons for translating GC methods to Hydrogen carrier including improved chromatography and faster throughput. The chromatographic benefits of adopting modern column geometries in conjunction with Hydrogen carrier gas will also be discussed.  We will highlight a very practical approach to method translation based on experience, and we will feature many tools and calculations which can be used to ease the translation experience, and maximize the benefits!  We will discuss regulatory challenges, such as, the need for method re-validation and approaches to verifying translated methods.

  • Key reasons and benefits of translating GC methods to Hydrogen Carrier
  • Direct translation or to improve speed and efficiency?
  • Tools and calculations to help with translation
  • What needs to be done – a practical checklist
  • Case study examples – avoiding pitfalls and maximizing benefits
  • Is method re-validation required?

Principles of GC Column Selection l Webcast & Tutorial

Thursday 20th February 2014

Principles of GC Column SelectionWhilst the choice of GC columns may seem myriad, the number of chemistries is actually rather limited, and in this webcast you will learn how to select the best stationary phase composition for your application.  We also consider the factors which should influence your selection of column dimensions, and highlight how to avoid wasting time and money, as well as maximizing your chances of a successful separation!  The Essential Guide from LCGC’s CHROMacademy presents the definitive guide in GC column selection.  In this session, Dr. John Hinshaw (Senior Scientist , BPL Global Ltd) and Dr. Dawn Watson (Technical Specialist, Crawford Scientific) explore the various factors associated with GC stationary phase selection. 

  • GC column selection is critical to obtaining the correct separation selectivity – what factors influence stationary phase choice?
  • Whilst choosing a ‘generic’ phase can bring satisfactory results, there are often better choices which will improve specificity and sensitivity
  • GC column dimensions directly influence the quality of a separation - learn how to choose the best column length, internal diameter, and film thickness for your application
  • Learn how to optimize your separation through smart column selection
  • Appreciate when to stop optimizing method conditions and start choosing a different column!

HPLC Detectors – What, Where, When, and How l  Webcast & Tutorial

Thursday, January 23rd 2014, 16:00 GMT

HPLC Detectors The first Essential Guide of the new year will focus on the operating principles of the most common HPLC detectors. The parameters that should be optimized for each detector type will be examined to give the optimum performance from the chosen detector. The advantages, disadvantages, and application areas for each detector type will also be discussed. This in depth review of HPLC detectors will result in a better understanding of the operation, optimization, and detector choice for every application.

  • What detectors are available for HPLC applications
  • When and why would you choose one detector over another
  • What are the advantages and disadvantages of each detector type
  • Which detector parameters require optimization and what is their impact?
  • Is there an ideal detector and if not what would it look like?

Automated Derivatization for Gas Chromatography l Live Webcast

Tuesday, January 28th 2014

Automated Derivatization for Gas Chromatography Andrea Caruso (GC Applications Chemist, Thermo Fisher Scientific) and Tony Taylor (Technical Director, Crawford Scientific and CHROMacademy) demonstrate the power of analyte derivatization to improve Gas Chromatography performance. Reactions will be highlighted to improve volatility, improve peak shape and quantitative reproducibility, improve selectivity and specificity with traditional and MS detection. They will also highlight recent technology advances which make a host of derivatization reactions suitable for automation, which enable just in time or unattended operation and which improve reproducibility of derivatization chemistry.

  • Derivatization has many benefits for dealing with difficult samples for Gas Chromatography and Mass Spectrometry
  • Learn the benefits which can be derived to deal with less volatile samples, those which produce poor chromatographic response or when sensitivity or specificity need to be improved
  • Real life case studies to highlight the benefits of derivatization
  • See how automation of derivatization reactions is now a real possibility for Gas Chromatography

Developing Better GC methods - a blueprint l  Webcast & Tutorial

Thursday, December 19th 2013

Developing Better GC methods - a blueprint This month we explore what constitutes the optimum GC method. We will examine the many instrument parameter settings, as well as the chemistry and temperature program considerations which can and should be optimized for every analysis. This webcast analyzes what the ideal method specification might look like and highlights the important decisions which lead to highly stable and sensitive GC methods. The webcast will draw up a blueprint for a highly useful and practically relevant optimum GC method.

Topics covered include:

  • What does the optimum GC method look like?
  • What instrument settings and chemistry choices must be considered in order to design the ideal GC method
  • This webcast highlights all of the decisions required to build a rugged and sensitive GC method
  • All sample, sample inlet, column, temperature programming and detector settings are considered in this highly informative and practically relevant webcast
Understanding GC Methods »
Thursday, October 24th 2013


Understanding GC Methods

The Essential Guide from LCGC’s CHROMacademy presents the first in a series of webcasts aimed to develop the skills and understanding of GC users.

In this session, we take an in-depth look at principles of GC sample introduction and the associated variables which need to be defined in GC methods. 

We describe the principles of sample injection and describe in detail how method variables are set and optimized using real life ‘good’ and ‘bad’ method examples.


  • Understand the working principles of the Split / Splitless Injector
  • Appreciate the major and minor variables which control sample injection
  • Recognize when a method is deficient in terms of the amount of information given and make informed choices about the parameters which are not stipulated
  • Appreciate the importance of proper inlet function and make informed choices in terms of inlet liners
  • Understand how to set and optimize sample injection variables based on sample (analyte) type and intended analytical outcomes

 

 

Characterization of Protein Biopharmaceuticals »
Tuesday, October 15, 2013 at 11:00 am EDT


Characterization of Protein BiopharmaceuticalsProtein biopharmaceuticals such as monoclonal antibodies and recombinant proteins are currently in widespread use for treatment of various life-threatening diseases including cancer, autoimmune diseases, diabetes, anemia, etc. Protein therapeutics have a complexity far exceeding that of small molecule drugs, hence, unraveling this complexity represents an analytical challenge. This web seminar will highlight the power of liquid chromatography combined with mass spectrometry in the characterization of protein biopharmaceuticals.

 

Topics covered include:

  • Chromatographic and mass spectrometric tools and strategies used in the characterization of protein biopharmaceuticals
  • Novel trends in biopharmaceutical analysis
  • Real-life examples on marketed biopharmaceuticals and therapeutics in development including biosimilars and biobetters

 

 

 

Developing Better HPLC (MS) MethodsDeveloping Better HPLC (MS) Methods »
26th September 2013, 16:00 GMT

Where do you start when you want to develop a better HPLC method? Whether you work in a regulated environment or not, setting specifications for your HPLC protocols is a good practice. In this Essential Guide we will set out guidelines for chromatographic parameters such as retention, resolution, and efficiency that can be used to improve your HPLC methods prior to method validation. We will explain how mobile phase design and instrument parameters can be used to achieve improvements in method robustness and what to look for when a method is failing. Practical tips for sample and eluent preparation, and the correct detector settings to use will also be discussed.

Topics covered include:

  • What target specifications should be set for an HPLC method
  • How sample preparation can impact on the sensitivity and robustness of an HPLC method
  • Stationary phase chemistry and important column properties
  • Designing ‘smart’ eluent solutions
  • Detector parameters to improve quality and robustness
  • Developing inherently robust methods
  • Aspects of LC-MS methods which can (and should!) be optimized

Critical Evaluation of HPLC MethodsCritical Evaluation of HPLC Methods »
19th September 2013 - 11am EST / 4pm GMT

There are numerous factors that affect the quality of your HPLC separation; including mobile phase composition, temperature, ionic strength, pH, column dimensions, and stationary phase and support material characteristics.  These can all be manipulated to change and improve your HPLC separations.  However, what are the changes we should expect?  Will retention improve if another solvent is used and would we expect selectivity to change?  Will certain analytes elute in the same order if the mobile phase pH, ionic strength, additives or temperature are changed? 

Topics covered include:

  • The effect of mobile phase polarity on HPLC separations
  • The role of a buffer in an HPLC separation and how it should be chosen
  • The effect of temperature on analyte retention and selectivity
  • Additives for improved chromatography
  • Stationary phase and support material chemistry and important properties
  • The effect of column dimensions on gradient elution
  • Why and when you would use an guard column or in-line filter
  • Detectors – advantages, disadvantages, & applications

The analysis of post-translational modifications using LC-MS/MSThe analysis of post-translational modifications using LC-MS/MS »
22nd August 2013 - 11am EST / 4pm GMT

In this session, Dr Duncan Smith and Dr John Griffiths (MS-Insight Ltd and Manchester University) explain the principles behind the analysis of a number of key post-translational modifications (PTMs) using LC-MS/MS techniques. They will begin with a brief overview of bottom-up proteomics, explaining how proteins are converted to peptides, which are then analysed using electrospray LC-MS/MS.

Topics covered include:

  • What is proteomics and why are post-translational modifications important?
  • How are proteins identified using LC-MS/MS?
  • Utilizing different MS scanning functions to enable PTM detection
  • How to detect both global and targeted phosphorylation events.
  • Approaches to acetylation studies
  • How non-classical tryptic proteolysis of SUMOylated proteins enables their facile detection
  • Novel approaches for ubiquitination and SUMO determination
  • The future – combinatorial PTM detection with quantification?

GC-MS/MSTranslating GC and GCMS Methods »
18th July 2013 11am EST / 4pm GMT

In this session we cover the various reasons for translating methods including improved chromatography, faster throughput, adopting modern column geometries and switching to hydrogen carrier from helium. We explore all aspects of instrument hardware and method variable translation and you will leave with a ‘toolkit’ approach for translating your own methods – saving you time, money and improving your chromatography!

Topics covered include:

  • Key reasons and benefits of translating GC and GCMS methods
  • Translating for speed, efficiency or to hydrogen from helium
  • Maintaining retention time
  • Avoiding selectivity changes in temperature programmed GC
  • Effects of changing carrier on detector performance & sensitivity
  • MS detector considerations

 

HPLC Troubleshooting Masterclass »
20th June 2013 11am EST / 4pm GMT

HPLC Troubleshooting Masterclass »This month we will use your chromatograms to help illustrate the optimum approach to troubleshooting HPLC problems. Using real world chromatograms submitted by CHROMacademy members from many different application areas, we illustrate how to recognize problems and explain further tests and investigations necessary to properly qualify the issue. 

Topics covered include:

  • Learn how experts troubleshoot HPLC chromatography problems
  • A series of real chromatograms are used to demonstrate logical ways to approach various issues in HPLC
  • Learn what problems and symptoms are important and how to further investigate issues
  • Learn what further tests can be done to better understand problems and fix issues more quickly
  • Learn to recognize problems quickly and achieve better data quality and higher throughput

GC-MS/MSGC-MS/MS Analysis – What you need to know! »
23rd May 2013 11am EST / 4pm GMT

In this session, we discuss the key features of GC-MS/MS instrumentation alongside an overview of instrument operating principles and theories. Various means of deriving MS/MS spectral information will be discussed with reference to the analyzers which make these experiments possible and key applications of MS/MS technology will be highlighted to demonstrate practical relevance and show what GC-MS/MS technology can do for you!

Topics covered include:

  • Overview of GC-MS techniques
  • Anatomy of GC-MS/MS instruments
  • Comparison or various MS/MS detectors
  • MS/MS experiment types
  • GC-HRMS,  GCxGC–MS/MS and Fast GC-MS/MS
  • What can GC-MS/MS do for me?
  • Applications of GC-MS in Forensics / Environmental, Food and Metabolomics

 

Core Shell Particles – Present and Future »
30th April 2013 11am EST / 4pm GMT

Core Shell ParticlesIn this session, Dr Fabrice Gritti and Scott Fletcher (Technical Manager, Crawford Scientific) highlight the benefits of core-shell particle technology and the reasons behind their surprisingly high efficiency.

Topics covered include:

  • What are the practical benefits of core-shell particles
  • What’s the real secret behind their high efficiency
  • Will core shell’s high efficiency lead to a reduction in the number of stationary phases required?
  • Why can’t we realise the true benefits of core-shell’s in narrow columns
  • Practical limitations of core-shell particles and core to shell thickness ratio
  • Sub-2μm core-shell particles – what’s the real benefit?
  • Core-shell’s for preparative chromatography
  • The future – what can we expect from these particles?

 

Mixed Mode Chromatography – the answer to everything? »
28th March 2013 11am EST / 3pm GMT

GC Troubleshooting MasterclassIn this session, Dr Xiaodong Liu (R&D Manager, Thermo Fisher Scientific) and Scott Fletcher (Technical Manager, Crawford Scientific) consider the technique of mixed mode chromatography and the application areas in which this technique can help chromatographers. The mechanisms of mixed-mode chromatography are discussed alongside the parameters which can be used to optimize selectivity and retention. A series of useful illustrative example are presented with strategies for colum selection and screening to allow this technique to quickly become part of the analytical armory!

 

Topics covered include:

  • What is mixed mode chromatography? - evolution and fundamental retention mechanisms
  • Mixed mode separations – comparison with reversed phase, normal phase ion exchange and HILIC chromatography
  • Suitable analytes / applications for mixed mode chromatography
  • Column Selection
  • Retention and selectivity control using organic modifier, buffer strength and pH
  • Screening and initial method development
  • Interesting illustrative examples
  • The future for mixed – mode phases

GC Troubleshooting Masterclass »
Thursday 21st February 2013, 11:00 AM EST, 16:00 GMT

GC Troubleshooting MasterclassDr John Hinshaw and Tony Taylor demonstrate practical GC separation troubleshooting. Using real world chromatograms submitted by CHROMacademy members from many different application areas, they illustrate how to recognize problems and explain further tests and investigations necessary to properly qualify the issue. They then offer advice on how the problem may be resolved to help you gain valuable insight into recognizing, characterizing and fixing problems in GC to improve your data quality and reduce instrument downtime...

 

Practical GC Troubleshooting:

  • Learn how experts troubleshooting GC chromatography problems
  • A series of real chromatograms are used to demonstrate logical ways to approach various issues in GC
  • Learn what problems and symptoms are important and how to further investigate issues
  • Learn what further tests can be done to better understand problems and fix issues quicker
  • Learn to recognize problems quickly and and achieve better data quality and higher throughput

 

 

webcast and tutorialLCMS Method Parameters - Critical Evaluation »
Thursday 28th February 2013, 11:00 AM EST, 16:00 GMT

In this session Arindam Roy (Associate Director, Quality, Novartis Consumer Health) and Scott Fletcher (Technical Manager, Crawford Scientific) will consider a range critical LC-MS method parameters and their impact on analytical sensitivity and specificity. The importance and impact of specific eluent chemistry, interface parameters, analyzer settings and types are all critically reviewed and evaluated in this highly relevant presentation.

 

Critical Evaluation of LCMS method parameters –
what does your method say about you!

  • Ever wondered why methods developed for LC-MS use TFA, TEA and other ion-pair reagents?
  • Ever wondered about the effects or organic modifier, buffer concentration or gradient analysis on LC-MS sensitivity?
  • Is there really a need to split column effluent flow on modern instruments?
  • Interested in how changing source voltages and parameters could effect ionization?
  • Ever questioned why specific scan times or ion dwell times are employed?
  • Interested in the benefits of using high accuracy and/or high resolution mass analyzers?
  • Are there any new column technologies that facilitate LC-MS analysis?

Optimizing LC-MS and LC-MS/MS »
Thursday 24th January 2013, 11:00 AM EST, 16:00 GMT

In this session we consider the major instrument and method variables in LC-MS analysis as well as some of the more minor ones which can sometimes make a big difference to your analysis. Eluent chemistry, interface parameters and analyzer settings are all considered.

 

Topics covered include:

    webcast and tutorial
  • What HPLC eluent factors are important in optimizing response in LC-MS
  • What everyday factors in HPLC can be easily changed to increase sensitivity or
    improve separation in LC-MS
  • What API interface parameters really make a difference to LC-MS sensitivity or specificity
  • What MS parameters can be adjusted to improve analysis quality
  • What can your analyser do for you!
  • Ion suppression and enhancement – an insurmountable problem?

 

The Secrets of Optimizing GCMS Methods »
Thursday 13th December 2012, 11:00 AM EST, 16:00 GMT, 5:00 PM BST

The definitive guide to optimizing methods for GCMS analysis.
In this session we consider the major instrument and method decisions that allow you to get the very best from your analysis.

 

Topics covered include:

    webcast and tutorial
  • GC column, flow rate and sample prep. considerations
  • Auotune - why and when
  • Improving your instrument tuning performance
  • Optimizing EI and CI source variables
  • Quadrupole – tuning for specific analytes
  • Spectral data acquisition – overlooked parameters
  • Optimizing methods for quantitative or qualitative analysis

 

Introduction to Ion Chromatography »
29th November 2012, 11:00 AM EST, 16:00 GMT, 5:00 PM BST

This guide considers the underlying theory of the technique, column and eluent selection and key separation variables as well as the various techniques used for ion suppression and detection. We also consider various applications of ion chromatography together with recent advances in the technique including capillary ion chromatography, fast and ultra high pressure ion chromatography as well as hyphenation of ion chromatography to mass spectrometric detectors.

 

Topics covered include:

    webcast and tutorial
  • Separation and detection
  • Post column suppression
  • Detector types
  • Eluent preparation strategies
  • Detection strategies
  • Column loading
  • Temperature effects
  • Typical buffers
  • Altering elution times and selectivity
  • Quantitative aspects
  • Fast ion chromatography
  • Capillary ion chromatography

 

Translating GC Methods from Helium to Hydrogen Carrier Gas »
October 18, 2012 | 11:00am EST | 16:00 BST

The definitive guide in translating your GC methods for use with a Hydrogen Carrier . We consider the use of hydrogen as an alternative carrier gas to Helium and present the facts around Hydrogen safety and use within the laboratory. We study method translation, including all of the important method and instrument parameters to ensure retention, selectivity and resolution are maintained. We consider the use and limitations of hydrogen carrier with mass spectrometric detectors and finally highlight some of the advantages of working with a carrier capable of producing high efficiency at higher flow rate.

 

Topics include

    Hydrogen
  • Why change at all?
  • Using hydrogen safely – generators and cylinders
  • Maintaining retention time
  • Avoiding selectivity changes in temperature programmed GC
  • Effects of changing carrier on detector performance & sensitivity
  • Using hydrogen with MS detectors
  • Re-optimizing methods for speed & efficiency

 

Translating and Transferring HPLC Methods
Thursday 20th September, 11:00 AM EST, 16:00 GMT, 5:00 PM BST
View the archived Webcast & Tutorial here

The Essential Guide from LCGC’s CHROMacademy presents an informative session on method translation and transfer in HPLC. In this session, Tony Taylor (Technical Director,Crawford Scientific) and Scott Fletcher (Business Development Manager, Crawford Scientific), present a definitive guide on everything that one woul;d need to consider for translating and transferring HPLC methods.

The session includes in-depth consideration of the hardware and data acquisition requirements for simple method transfer or translation to different column / particle size and morphology. We present many worked examples of calculations, approaches and pitfalls associated with method scaling and transfer and include up to date concepts such as frictional heating and pressure based selectivity to ensure you are fully up to date in this subject area. A must see for everyone translating or transferring HPLC methods.

 

hplc to uhplcTopics include

  • Instrument components important for method transfer
  • Characterizing your system for simple method transfer or translation to faster methods
  • Geometric scaling of method parameters (flow rate / loading etc.) for different columns / particle sizes
  • Translating gradients – pitfalls and tricks
  • UHPLC to HPLC – because sometimes we have to!
  • Pressure and Temperature effects on Selectivity

 

Troubleshooting GC Separation Part II – Selectivity, Resolution and Baseline Issues
Thursday 16th August 2012, 16:00 GMT

View the archived Webcast & Tutorial here

 

The Essential Guide from LCGC’s CHROMacademy presents the fourth and last in our series of webcasts on Practical GC Troubleshooting.  In this session, Dr. John Hinshaw (Senior Scientist , BPL Global Ltd) and Tony Taylor (Technical Director, Crawford Scientific), present practical troubleshooting tips for GC separations, with special emphasis on selectivity changes, loss of resolution and baseline problems.  The session includes a wealth of practical information on GC separation issues and all our real world problems are related to both separation chemistry and hardware issues.   We consider the common problems and highlight causes and remedial actions in a very practically relevant way.  A must see for everyone working with GC instruments.

Troubleshooting GC Separation

Topics include

  • What causes selectivity changes in GC – both sudden and over time
  • What are the main causes of loss of resolution within a separation and what are the signs and symptoms to look out for
  • Typical problematic baselines and their causes
  • Learn how to quickly identify problems and investigate the root cause
  • Build up a portfolio of preventative and corrective maintenance operations

 

Troubleshooting your HPLC Chromatogram Part II - Selectivity | Resolution | Baseline Issues.
July 19th 2012, 11:00am EDT, 4.00pm BST.

View the archived Webcast & Tutorial here

 

In this session, Dr. Dafydd Milton  (Product Manager, Thermo Fisher Scientific) and Scott Fletcher (Technical Manager, Crawford Scientific), examine the common causes of changes in selectivity, loss of chromatographic resolution and baseline issues – such as splitting, fronting, tailing and shouldering.

Strategies for problem identification and calculation of critical chromatography performance indicators will be discussed. The degree to which these parameters are affected by mobile phase composition, temperature, column modification and age, sample solvent strength, sample preparation, system operation and many other variables will be investigated alongside strategies for isolating the precise cause of the problem.

Corrective chromatographic and / or HPLC system actions as well as key preventative maintenance operations will be described for the major causes of the symptoms observed.  Strategies for identifying the causes of problems with current methods and best practice for future method development are also included.

 

autosamplerTopics include

  • Identify common causes of problems within your chromatography
  • Learn logical strategies for rapid instrument and separation troubleshooting
  •  Explore common problems with
    •  Mobile phase composition
    • Temperature and pH
    • Column modification and age
    • Sample solvent strength
    • Sample preparation
    • System operating parameters
  • Understand issues with existing problematic methods
  • Instigate preventative steps in order to minimize problems in future methods

 

Troubleshooting GC Separations.
June 14th 2012, 11:00am EDT, 4.00pm BST.

View the archived Webcast & Tutorial here

 

Troubleshooting GC SeparationsThe Essential Guide from LCGC’s CHROMacademy presents the third in our series of webcasts on Practical GC Troubleshooting. In this session, Dr. John Hinshaw (Senior Scientist , BPL Global Ltd) and Tony Taylor (Technical Director, Crawford Scientific), present practical troubleshooting tips for GC separations, with special emphasis on retention time variability, loss of efficiency and poor peak shape.

The session includes a wealth of practical information on GC separation issues and all our real world problems are related to both separation chemistry and hardware issues. We consider the common problems and highlight causes and remedial actions in a very practically relevant way. A must see for everyone working with GC instruments.

 

Topics include

  • Investigate causes of retention time variability in GC including carrier and injection issues
  • Investigate tailing, fronting and shouldering peak behaviors – identify the major causes and learn practical remedies for the problem
  • Learn about loss of efficiency in capillary GC and identify the major causes, including injection and column related issues
  • Learn how to quickly identify problems and investigate the root cause
  • Build up a portfolio of preventative and corrective maintenance operations

HPLC Troubleshooting Separations Retention Time Efficiency Peak Shape Issues Essential Guide
May 2012
View the archived Tutorial here

 

This essential guide examines the common causes of retention time drift, loss of chromatographic efficiency and peak shape issues – such as splitting, fronting, tailing and shouldering. Strategies for problem identification and calculation of critical chromatography performance indicators will be discussed.

The degree to which these parameters are affected by mobile phase composition, temperature, sample solvent strength, and many other variables will be investigated alongside strategies for isolating the precise cause of the problem.

Corrective and preventative actions will be described for the major causes of the symptoms observed. In Part II, July’s Essential Guide, we will focus on Selectivity, Resolution and Baseline issues.

 

hplc troubleshootingTopics include

  • Proper column installation and conditioning
  • Capillary column performance degradation
  • Protecting the column and prolonging column lifetime
  • Detector principles (FID / NPD / ECD / TCD)
  • Detector performance optimization
  • Troubleshooting detector response / sensitivity issues
  • Maintenance Schedule

 

 

GC Troubleshooting - Column & Detector Issues
April 24th 2012, 11:00am EDT, 4.00pm BST.
View the archived Webcast & Tutorial here

 

The Essential Guide from LCGC’s CHROMacademy presents the second in our series of webcasts on Practical GC Troubleshooting. In this session, Dr. John Hinshaw (Senior Scientist , BPL Global Ltd) and Tony Taylor (Technical Director, Crawford Scientific), present practical troubleshooting and maintenance information associated with capillary GC columns and common detectors. The session includes a wealth of practical information on avoiding issues when preparing, installing and conditioning capillary GC columns. We also consider causes of capillary column aging and how to ensure your column remains fit for purpose for as long as possible! Also included are a quick revision on common GC Detectors (FID / TCD / ECD / NPD) as well as practical tips for avoiding problems and optimizing detector response. We conclude by considering the symptoms of detector problems and building a list of troubleshooting and maintenance items which will be useful to the GC practitioner. Throughout, we consider both instrument and chromatographic symptoms. A must see for everyone working with GC instruments.

 

gc columnTopics include

  • Proper column installation and conditioning
  • Capillary column performance degradation
  • Protecting the column and prolonging column lifetime
  • Detector principles (FID / NPD / ECD / TCD)
  • Detector performance optimization
  • Troubleshooting detector response / sensitivity issues
  • Maintenance Schedule

 

HPLC Troubleshooting - Autosampler, Column & Detector Issues
Wednesday 28th March 2012, 11:00am EDT, 4.00pm BST.
View the archived Webcast & Tutorial here

 

The Essential Guide from LCGC’s CHROMacademy presents the second in our series of webcasts on Practical HPLC Troubleshooting.  In this session, Dr. Kevin Schug (Associate Professor, University of Texas, at Arlington) and Scott Fletcher (Technical Manager, Crawford Scientific), present practical troubleshooting and maintenance information associated with HPLC autosamplers, columns and detectors.  This session examines different autosampler designs, injection valve operations and common problems associated with modern HPLC autosamplers.  Sample solvent and injection volume effects on chromatography will also be discussed in detail.  We will consider column hardware design, materials of construction and associated issues – including minimizing dead volume and avoiding / troubleshooting column blockage issues, especially with the popular Sub 2µm column packing materials.  We will also investigate the importance of controlling the temperature of the column during analysis and the potential chromatographic effects of poor temperature control.  We will conclude by reviewing common HPLC detector hardware problems and the chromatographic issues associated with detector hardware and acquisition settings.   We will build a checklist of suggested maintenance operations as well as outlining common diagnostic tests.

 

Topics include

  • Autosampler designs, common problems and avoiding carryover
  • Sample solvent and injection volume effects
  • Column hardware design, construction and mechanisms of column blockage
  • Column temperature control issues
  • Common HPLC Detector problems
  • Chromatographic symptoms and diagnostic tests
  • Common maintenance operations

 

 

The CHROMacademy Essential Guide to GC Troubleshooting - Gas Supply and Inlet Issues
View the archived Webcast & Tutorial here

 

The Essential Guide from LCGC’s CHROMacademy presents the first in our series of webcasts on Practical GC Troubleshooting. In this session, Dr. John Hinshaw (Senior Scientist , BPL Global Ltd) and Tony Taylor (Technical Director, Crawford Scientific), present practical troubleshooting and maintenance information associated with gas supply and management, autosamplers and split / splitless sample inlets. This session considers good practice for gas supply and delivery, electronic pressure control issues and gas quality management. We also investigate common problems and mistakes in choosing sample solvents and issues with autosamplers and syringes. We consider the most common instrument problems and mistakes associated with split / splitless inlets and examine essential maintenance to help avoid the pitfalls. We include examples of the impact of all issues considered on chromatography and quantitative data analysis and consider various troubleshooting tests and diagnostics that might be used. A must see for everyone working with GC instruments.

 

Topics include

  • Gas supply management and good practice
  • Pressure management issues
  • Sample preparation and autosampler problems
  • Common Split / Splitless inlet problems
  • Chromatographic, instrument and quantitative symptoms
  • Diagnostic tests
  • Common gas supply and inlet maintenance operations

 

 

HPLC Troubleshooting - Eluents and Solvent Delivery Systems
View the archived Webcast & Tutorial here

 

The Essential Guide from LCGC’s CHROMacademy presents the first in our series of webcasts on HPLC Troubleshooting.  In this session, Scott Fletcher (Technical Manager , Crawford Scientific) and Tony Taylor (Technical Director, Crawford Scientific), present practical troubleshooting and maintenance information around HPLC eluents and solvent delivery systems (pumps).  This session considers important practical concepts such as good practice for eluent preparation, filtration and degassing as well as common related issues such as retention time drift and selectivity changes.  We also explain the working principles of HPLC solvent delivery systems and highlight the components which are most susceptible to problems.  The webcast concludes by considering various trohbleshooting tests and strategies as well as common maintenance operations for solvent delivery systems. A must see for anyone who uses HPLC equipment and who wants to improve their troubleshooting and instrument maintenance skills.

 

baseline profileTopics include

  • Good practice for eluent preparation
  • Troubleshooting retention time and selectivity issues
  • Degassing – do’s and don’ts
  • Anatomy of a mobile phase delivery system
  • Components that go wrong and why
  • Symptoms of a poorly pump
  • Troubleshooting and tests
  • Recommended maintenance operations

Mass Analyzers – Traps and TOF’s
View the archived Webcast & Tutorial here

 

The Essential Guide from LCGC’s CHROMacademy presents the second in our series of webcasts on Mass Analyzers.  In this session, Dr. John Langley (Head of Mass Spectrometry, University of Southampton, UK) and Tony Taylor (Technical Director, Crawford Scientific), present the fundamentals of Ion Trap and Time of Flight, mass analyzers supported by Interactive Multi-Media from The CHROMacademy.  The session will consider topics such as the anatomy of the analyzer types, working principles, the importance of mass accuracy and resolution, when to use the various analyzer types and the performance one might expect from each.  A must see for everyone working, or hoping to work, with Ion Traps, Linear Ion Traps, Orbitraps or Time of Flight instruments.

 

Topics include

  • Why use an Ion Trap or Time of Flight mass analyzing Device
  • Anatomy of the analyzers
  • Importance of analyzer accuracy and resolution
  • Two and three dimensional Ion Traps
  • Orbitrap analyzers
  • Mass analyzing principles
  • Important operating parameters
  • Mass spectral experiments
  • Pro’s and con’s of the mass analyzers
  • Figures of merit
 

 

Quadrupole Analyzers and MS/MS Analysis
View the archived Webcast & Tutorial here

 

The Essential Guide from LCGC’s CHROMacademy presents an educational webcast on Quadrupole Analyzers and MS/MS Analysis. In this session, Dr. Kevin Schug (CHROMacademy LC Department Dean) and Scott Fletcher (Technical Business Development Manager, Crawford Scientific), present a definitive guide to the theory, instrumentation and application of this now common place technique. The session will initially consider the need for mass analyzers before concentrating on the quadrupole, which has become a mainstay for the majority of analytical laboratories. The impact the quadrupole design, geometry and applied voltages have on ion stability will be investigated. Special attention will be reserved for the electrostatic fields created within the quadrupole and the resulting equations of motion. Quadrupole resolution, sensitivity and mass accuracy will also be considered. We will then continue to review the additional benefits and design changes required in order to operate quadrupoles in tandem (MS/MS). Typical MS/MS experiments will then be reviewed individually and their specific operation and benefits presented and explained.

 

Mass AnalyzersTopics include

  • Why are Mass Analyzers required?
  • Introduction to Quadrupole Mass Analyzers
  • Quadrupole Design and Orientation
  • Quadrupole Voltages and Electrostatic Fields
  • Stable and Unstable / Collisional Trajectories
  • Equations of Motion
  • Quadrupole Resolution and Sensitivty
  • Scan vs. Selected Ion Monitoring
  • Quadrupole Mass Accuracy
  • RF only Ion Bridges
  • Introduction to Tandem Quadrupole (MS/MS) Mass Analyzers
  • Benefits of MS/MS Mass Analyzers
  • Design of MS/MS Mass Analyzers
  • Typical MS/MS Experiments

 

Basics of Preparative HPLC

View the archived Webcast & Tutorial here

 

The Essential Guide from LCGC’s CHROMacademy presents an educational webcast on the Basics of Preparative HPLC.  In this session, Dr. Charles White (YMC Europe GmbH, UK Operations) and Tony Taylor (Technical Director, Crawford Scientific), present an introductory guide to the theory, instrumentation and applications of Preparative HPLC.  The session will consider all the relevant aspects of theory and practical implementation and will consider the requirements imposed at analytical; scale when considering scale-up to prep.  We will also consider logical approaches to scaling an analytical separation with target recoveries in mind as well as highlighting some of the major pitfalls of the technique and practical tips to overcome them. Throughput and method robustness will also be considered and are factors that are often overlooked by the novice preparative HPLC user.

 

Topics include:

  • Prep HPLCUses of preparative chromatography
  • Column Considerations
  • Preparative Separation Goals
  • Analytical separations designed for preparative applications
  • Method development
  • Optimizing loading at analytical scale
  • Volume & Concentration Overloading
  • Sample Solvents
  • Methods of Scale Up
  • Scalable and non-scalable factors
  • Productivity and throughput
  • Robustness and method verification

Understanding 2D Gas Chromatography
View the archived Webcast & Tutorial here

 

The Essential Guide from LCGC’s CHROMacademy presents an educational webcast on Comprehensive Two Dimensional Gas Chromatography. In this session, Dr. John Hinshaw (CHROMacademy GC Department Dean, GC Column Connections Editor) and Tony Taylor (Technical Director, Crawford Scientific), present a definitive guide to the theory, instrumentation and applications of this now fully established technqiue. The session will consider the need for multidimensional separations in gas chromatography and the application areas in which this technique has found great utility, as well as the various means by which 2D serial column C can be achieved. We will consider the instrumentation required for the comprehensive 2D technique, with special emphasis on column stationary phase and dimensions as well as the design and role of the ‘modulator’ within the system. Detection and data treatment are also carefully considered as these are often the ‘driving force’ behind the successful implementation of the technique form both a qualitative and quantitative standpoint.

 

2D Gas ChromatographyTopics include:

  • Why are multidimensional separations required?
  • Peak capacity in multidimensional GC
  • Typical Application Areas
  • Comprehensive v’s other multidimensional serial column techniques
  • Instrumentation Overview
  • Traps and modulators – design and important practical aspects
  • Stationary phase and column dimension choices
  • MS & other detectors for multidimensional GC
  • Data reduction / quantitative analysis

 

Introduction to Ion Chromatography
View the archived Webcast & Tutorial here

 

Applicability of selected forms of liquid chromatographyThe Essential Guide from LCGC’s CHROMacademy present an educational webcast on the fundamental principles of ion chromatography. In this session, Dr. Ken Cook (Bio - Separations Manager, Dionex) and Tony Taylor (Technical Director, Crawford Scientific), present a definitive guide to the fundamental theory and instrumentation of ion chromatography.

This guide considers the underlying theory of the technique, column and eluent selection and key separation variables as well as the various techniques used for ion suppression and detection. We also consider various applications of ion chromatography together with recent advances in the technique including capillary ion chromatography, fast and ultra high pressure ion chromatography as well as hyphenation of ion chromatography to mass spectrometric detectors.

Topics include:

  • Basic theory of ion chromatography
  • The ion chromatography syste
  • Column choice and basic applications of ion chromatography
  • Eluents for ion chromatography
  • Advances ion chromatography
  • Hyphenating ion chromatography to mass spectrometry

 

Understanding HPLC Column Characterization and Selection:
View the archived Webcast & Tutorial here

 

The Essential Guide from LCGC’s CHROMacademy present an educational webcast on HPLC Column Characterization and Selection for reversed phase separations.  In this session, Dr. Tony Edge (Technical Manager, Thermo Scientific) and Tony Taylor (Technical Director, Crawford Scientific), present a definitive guide to the advanced classification of HPLC stationary phases.  The session will consider the critical characteristics of HPLC supports and bonded phase ligands for reversed phase HPLC and the way in which these materials are classified in terms of the essential performance characteristics by column vendors and independent testing organizations alike.  We will also consider how classification results can be used to select columns for HPLC method development and the relationships between analyte and column characteristics. A must see for everyone using or developing methods for reversed phase HPLC.

 

Topics include:

  • Essential characteristics of an HPLC stationary phase
  • Review of bonded phase chemistry
  • Why characterize HPLC columns?
  • Column classification databases
  • Common classification tests
  • Interpreting classification results
  • Using column classification to aid column selection
  • Relating column properties to analyte characteristics
  • Similar and orthogonal phases
  • Column classification – the future

 

Chromatographic Method Validation:
View the archived Webcast & Tutorial here

 

In this session, Dr. Mike Swartz (Ariad Pharmaceutical, Cambridge, MA) and Scott Fletcher (Technical Business Development Manager, Crawford Scientific), present an Introduction to Chromatographic Validation.  The session will consider topics such as why validate a chromatographic method?,  regulatory guidelines, eight steps of analytical method validation and a review of how and when to use statistical tests.  A must see for everyone working in a regulated environment requiring validated methods or those wishing to benefit from the increased confidence that method validation brings. The material in the tutorial reflects two excellent previous Validation Viewpoint articles from Mike and Ira Krull which can be found at the links below:

 

figure 4Topics include:

  • The Method Validation process
  • Guidelines on Implementing Analytical Method Validation (AMV)
  • Analytical performance characteristics - Accuracy, Limit of Detection, Limit of Quantification, Specificity / Selectivity, Linearity, Range, Robustness
  • Statistical tests used in AMV

 

 

Developing Fast Capillary GC Separations

View the archived Webcast & Tutorial here

 

The Essential Guide from LCGC’s CHROMacademy presents Developing Fast Capillary GC Separations. 
Dr. John Hinshaw (Senior Staff Engineer at Serveron Corp, CHROMacademy GC Dean) and Tony Taylor (Technical Director, Crawford Scientific), present a concise guide on increasing sample throughput for capillary GC for both existing standard equipment as well as the latest bespoke equipment and approaches for ‘Fast GC’.

Going to faster speeds of analysis with GC doesn't always require a new or upgraded instrument, but not all existing instruments are suitable. It all depends upon how fast a separation is required. A modest increase of two to four times shorter retentions using conventional capillary columns with inner diameters of 150 - 200 µm can be achieved quite reasonably on a wide range of conventional laboratory instruments.
Conversely very high analysis speeds can achieve separations that previously needed 10 min or longer in less than 1 min, but such a feat requires significant equipment upgrades or complete replacements. Pushing peaks from a column at high speeds places demands upon autosamplers, inlets, ovens, detectors, and data-handling systems that existing equipment might not be able to handle adequately.

This Essential Guide Tutorial will cover the most important practical issues to consider when increasing analysis speed using conventional equipment as well as taking a look at some of the newer equipment and methodologies required for ultra-high speed analysis.

 

This Essential Guide Tutorial will cover the most important practical issues when increasing analysis speed.
The table below shows John Hinshaws’ summary of the four proposed ‘speeds’ within capillary GC.

GC speed

Understanding Electron Ionization for GC-MS
View the archived Webcast & Tutorial here

 

The Essential Guide from LCGC’s CHROMacademy presents the second in our series of webcasts on Gas Chromatography-Mass Spectrometry (GC-MS).  In this session, Dr. John Langley (Head of Mass Spectrometry, University of Southampton, UK) and Tony Taylor (Technical Director, Crawford Scientific), present the fundamentals of Electron Ionization (EI) for GC-MS, supported by Interactive Multi-Media from CHROMacademy.  The session will consider topics such as the anatomy of a typical EI source, the mechanisms of ionization, fragmentation and re-arrangement reactions and an introduction to EI mass spectral interpretation. A must see for everyone using or developing methods for GC-MS.

 

simple-ei-sourceTopics include:

  • Why use Electron Ionization (EI)
  • Anatomy of a typical EI source
  • Vacuum and analyte introduction
  • Creation of radical cations
  • Energy of ionizing electrons
  • Transporting ions to the mass analyser
  • Analyte fragmentation and
    re-arrangement reactions
  • Tuning, Troubleshooting & Maintenance
  • Simple tools for EI interpretation

Electrospray Ionization (ESI) for LC-MS (Part 1)

View the archived Webcast & Tutorial here

 

ESI for LCMSThis month our industry experts help you to understand the fundamental basis of electrospray ionization mass spectrometry.  Learn how to optimize the essential parameters and improve instrument response and reproducibility in your lab.  Improve your electrospray data and troubleshoot your ionization and data interpretation problems more effectively.  Learn the fundamental basis of ion suppression so you can take best steps to avoid this phenomenon and discover best practice for acquiring qualitative data.

 

Topics include

  • Overview of ESI Hardware
  • When to use electrospray
  • Eluents and eluent flow rate
  • The nebulization process – key factors in optimizing instrument response
  • Desolvation processes - ensuring efficient droplet desolvation
  • Mechanisms for producing ions in the gas phase – optimizing sensitivity
  • Quantitative aspects of ESI – droplet and gas phase processes leading to ion suppression / enhancement

Hydrophilic Interaction Chromatography (HILIC) and related techniques (part 1)

View the archived Webcast & Tutorial here

 

Industry experts Dr Amos Heckendorf (The Nest Group) and Tony Taylor (Crawford Scientific & CHROMacademy), present a comprehensive review and tutorial on Hydrophilic Interaction Chromatography (HILIC). This technique is very useful for retention and separation of polar and ionisable compounds and provides a very useful orthogonal separation mechanism for chromatographers.


HILIC modeTopics include

  • Just what is HILIC?
  • Fundamental HILIC and Mixed Mode
    Retention Mechanisms
  • Optimizing Solvent Strength & Retention
  • The influence of pH
  • Buffers or additives or both?
  • Stationary Phases for HILIC and
  • Mixed Mode Chromatography
  • pH, eluotropic and buffer strength
    gradients
  • Practical Issues / Column Equilibration
  • Detection issues

 

 

Understanding Gradient HPLC

View the archived Webcast & Tutorial here

 

The Essential Guide from LCGC’s CHROMacademy presents the definitive practical guide for users of Gradient HPLC. In this session, Scott Fletcher and Tony Taylor (CHROMacademy Technical Director), present a definitive guide to using, optimizing and troubleshooting Gradient HPLC, all supported by Interactive Multi-Media from The CHROMacademy. The session will consider key topics such as the mechanisms of Gradient HPLC versus isocratic elution, developing robust gradient HPLC methods, the dangers of altering column flow or column dimensions without proper gradient scaling and the essentials of troubleshooting gradient issues. A must see for everyone using or developing Gradient HPLC methods.

A must see for everyone using or developing Gradient HPLC methods.

Measurement of Retention Factor (k) in Isocratic HPLC

 

Understanding GC-MS Analysis (Part I)

Watch the archived webcast   |   Tutorial

 

In this session, John Hinshaw and Tony Taylor , present the fundamentals of using gas chromatography with mass spectrometric detectors all supported by Interactive Multi-Media from The CHROMacademy. The session will consider key topics such as controlling and optimizing the vacuum system, ensuring good analyte transfer from the GC column, the theory and instrumentation of electron impact ionization and a detailed introduction to the quadrupole mass analyser. A must see for everyone using or developing methods for GC-MS.

Typical GC-MS System

 

Developing Better Methods for Reversed Phase HPLC (Part 2)

Watch the archived webcast   |   Tutorial  

 

Kevin Schug and Tony Taylor present more on logical optimization strategies for investigating and optimizing selectivity and resolution in reversed phase HPLC.

 

Topics covered include

  • Analytical Goals to Consider
  • Selecting the right HPLC Column
  • Where to begin?
  • Use of pH
  • Analytical Goals to Consider

 

Rapidly Becoming Confused! Superficially Porous or Sub 2µm Stationary HPLC Phases

Watch the archived webcast   |    Tutorial

 

High efficiency at high flow ratesMike Swartz and Scott Fletcher compare and contrast the relative advantages and disadvantages of employing HPLC columns packed with superficially porous or sub 2µm conventional fully porous particles

 

Topics covered include

  • High Efficiency Separations – Benefits and Plate Theory
  • Practical interpretation of the van Deemter Equation
  • High efficiency at high flow rates – practical approaches
  • UHPLC Instrumentation
  • Superficially Porous Silica Particles
  • SPS Particles – Advantages of Larger Particles for Facile Analysis

Understanding the Mechanisms of Solid Phase Extraction

Watch the archived webcast   |   Tutorial

 

High efficiency at high flow ratesThe Essential Guide from LCGC’s CHROMacademy presents the definitive webcast on Understanding Mechanisms in Solid Phase Extraction. In this session Tony Taylor (CHROMacademy Technical Director), presents the fundamentals of SPE protocol design, sorbent selection, optimization strategies for each protocol stage and topics of best processing practice all supported by Interactive Multi-Media from The CHROMacademy. The session will also consider key topics such as selection of the correct sorbent chemistry and bed mass, generic polymer resins, the importance of sample and sorbent preparation, strategies for optimizing selectivity through aggressive sorbent washing and optimized eluent strengths as well as some fantastic topics on improving extraction selectivity and efficiency. A must see for everyone using or developing methods for solid phase extraction.

 

Topics covered include

  • Selecting Sorbent chemistry (including Polymer sorbents)
  • Secrets of Sorbent Preparation
  • Sample preparation and pH matching
  • Optimizing analyte retention through proper sorbent equilibration
  • Optimizing wash steps for maximum selectivity
  • Overcoming fears in analyte elution!
  • Four things that everyone does incorrectly!
  • Facile processing secrets

Column Selection for Capillary GC

Watch the archived webcast   |   Tutorial

 

Typical Hydrogen Bonding InteractionsThe Essential Guide from LCGC’s CHROMacademy presents the definitive webcast on Column Selection for Capillary GC. In this session, John Hinshaw (GC Department Dean) and Tony Taylor (CHROMacademy Technical Director), present the fundamentals of GC phase design, bonding and deactivation as well as stationary phase selection using Interactive Multi-Media from The CHROMacademy. The session will also consider key topics such as column preparation, installation and conditioning as well as considering the effects of the column geometry (Length, Internal Diameter and Phase Thickness), on the separation. A must see for everyone using or developing methods for Capillary GC.

Understanding Stationary Phase Chemistry for Reversed Phase HPLC (Part I)
Watch the archived webcast   |   Tutorial

stationary phase

Topics covered include

  • Review of Reversed Phase Retention Mechanisms
  • Review of modern support (packing) materials
  • Important parameters of the packing material
  • Stationary Phase Chemistry Basics
  • RP stationary phases – matching phase to application
  • Silanol activity and silica surface treatments
  • Water wettable phases
  • Working outside the traditional pH ranges
  • Monolithic and Porous Graphitic Carbon Phases

 

Understanding and Optimizing Detectors for Capillary GC

Watch the archived webcast   |   Tutorial

 

Flame Jet ExitTopics covered include

  • Review of Common Detector Types for Capillary GC
  • Principles and Practice of:
  • Flame Ionization Detectors
  • Nitrogen Phosphorous Detectors
  • Electron Capture Detectors
  • Thermal Conductivity Detectors
  • Learn about gases, flow rates and parameter settings
  • Maximise your detector sensitivity
  • Effectively troubleshoot detector problems
  • Relate detector issues to chromatographic problems

 

Mobile Phase Optimization Strategies for Reversed Phase HPLC

Watch the archived webcast

mobile phase

Topics covered include

  • Review of Reversed Phase Retention Mechanisms
  • Methanol or Acetonitrile– which one is best?
  • Eluotropic Strength – quick ways to reach the ideal!
  • Changing Solvent – useful tools and approaches
  • Which buffer - what strength?
  • Mobile phase pH – understand the effects
  • Optimising pH v’s retention
  • Strategies for when all else fails


split splitlessSplit / Splitless Injection

Watch the archived webcast

 

Topics covered include

  • Injector components
  • Gas flows in Split and Splitless Mode
  • Critical operating parameters in Split and Splitless mode
  • Optimising injector settings for maximum sensitivity and repeatability
  • Inlet liners – the critical facts
  • Troubleshooting Inlet Hardware
  • Investigating Irreproducibility and Poor Peak Shape.

 




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