The fundamental principles of high performance liquid chromatography (HPLC) will be covered to begin your training. The fundamental chromatographic parameters which govern resolution within chromatography will be detailed.
We will then move on to discuss reversed phase HPLC, the most widely used mode of chromatography. The fundamental separation mechanism will be detailed along with discussions on the effect of mobile phase components and how to select the most appropriate eluent for a separation. Working with ionizable analytes requires specific considerations when designing an HPLC protocol such as eluent pH, appropriate buffer choice, and the possibility of unwanted secondary interactions within the HPLC column. All of these topics will be covered in order to give you a full understanding of this ubiquitous separation mode.
Dawn comes from an academic and commercial background. Her PhD and Postdoctoral research focus was in Inorganic chemistry. She has extensive experience in wet chemistry techniques and the use of a wide array of analytical techniques for reaction characterisation and monitoring; HPLC, LC-MS, IR, NMR, and GC. Her commercial experience was gained through the sales and support of automated instrumentation; HPLC, SPE, and LC-MS. Dawn’s experience has been gained with the following companies: Strathclyde University, Princeton University, and Gilson.
Associated Training Material from CHROMacademy
FAQs will be added as students progress through the course.
Tutor support for this session is provided by Dawn Watson (MSci (Hons), PhD).