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Fundamentals of HPLC Video Training Course
Session 2

 
 

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Session 2 Details

Selecting the correct HPLC column for your application is absolutely crucial.  This session will examine the available column stationary phases and support materials (including conventional fully porous and core-shell/superficially porous material), column dimensions and the effect that they can have on your chromatography.

Following this, the use of gradient elution in HPLC will be considered.  We will cover the fundamental principles of this technique which lead to the characteristic sharp chromatographic peaks produced by this elution mode.  We will demonstrate how to optimize essential gradient parameters using scouting gradients and simple calculations in order to allow you to quickly achieve suitable separation of analytes. 

Session 2   |  Runtime - 110 min.
  • HPLC Columns
  • Gradient Elution
session 1 details session 2 details session 3 details session 4 details

Course Overview »

Tutor support

Dawn Watson (MSci (Hons), PhD)

Dawn WatsonDawn comes from an academic and commercial background. Her PhD and Postdoctoral research focus was in Inorganic chemistry. She has extensive experience in wet chemistry techniques and the use of a wide array of analytical techniques for reaction characterisation and monitoring; HPLC, LC-MS, IR, NMR, and GC. Her commercial experience was gained through the sales and support of automated instrumentation; HPLC, SPE, and LC-MS. Dawn’s experience has been gained with the following companies: Strathclyde University, Princeton University, and Gilson.

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Associated Training Material from CHROMacademy

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FAQs

FAQs will be added as students progress through the course.

 
Tutor support for this session is provided by Dawn Watson (MSci (Hons), PhD).
 
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