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The CHROMacademy Essential Guide Webcast:
Everything you should know about UV detection for HPLC



Tuesday 19th November 2014,
8:00am PDT / 11:00am EDT / 16:00 BST / 17:00 CEST

There are many aspects of UV detection for HPLC which are poorly understood and could be improved or optimized in order to improve your analyses. Key information on the instrumentation and the use and optimization of UV detectors for modern HPLC applications will be discussed.  Fundamental UV theory will be revised with suggestions on how to choose optimum acquisition parameters based on analyte spectra and UV instrument design. Key practical concepts will be explored and the optimization of each acquisition variable will be explained in detail in terms of both the instrumentation and chromatographic data sensitivity and specificity.  Instrument limitations and dependencies will be discussed so that optimum method robustness might be obtained in everyday use.  Aspects of modern HPLC instrument design will be explored and related to improve chromatographic performance.

Presented by:
Professor Tony Edge (Thermo Fisher Scientific)
Tony Taylor (Technical Director, Crawford Scientific)

  • Review of fundamental UV absorbance theory
  • Understanding the optical arrangements of common UV detectors
  • Data acquisition parameter optimization – including bandwidth, slit width, and time constant
  • Detector linearity – limitations imposed by analyte spectra, bandwidth, and slit width settings
  • Choosing an appropriate reference wavelength to reduce gradient baseline drift and noise
  • Sensitivity – lamp effects, optical and electronic noise, solvent effects etc.
  • Flow cell design – modern light-pipe designs, illuminated volume, internal reflection principles and dispersion
  • Detection robustness - optimizing settings based on chromatographic data and improving your analytical robustness / sensitivity

Key Learning Objectives:

  • Understand / review fundamental UV-Visible spectroscopic theory
  • Appreciate the optical arrangement of Variable Wavelength and Diode Array detectors
  • Appreciate the importance of acquisition wavelength, bandwidth, slit width, reference wavelength and time constant settings
  • Relate UV acquisition settings to chromatographic sensitivity and robustness requirements
  • Understand the sources of noise and the limitations of linearity in UV detection and their effects on the validity and robustness of chromatographic data

Who Should Attend:

  • Anyone working with Liquid Chromatography
  • Anyone who wants to improve their analysis using UV detection
 
UV detection for HPLC
 

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  • Review of fundamental UV absorbance theory
  • Understanding the optical arrangements of common UV detectors
  • Data acquisition parameter optimization – including bandwidth, slit width, and time constant
  • Detector linearity – limitations imposed by analyte spectra, bandwidth, and slit width settings
  • Choosing an appropriate reference wavelength to reduce gradient baseline drift and noise
  • Sensitivity – lamp effects, optical and electronic noise, solvent effects etc.
  • Flow cell design – modern light-pipe designs, illuminated volume, internal reflection principles and dispersion
  • Detection robustness - optimizing settings based on chromatographic data and improving your analytical robustness / sensitivity

Who Should Attend:

  • Anyone working with Liquid Chromatography
  • Anyone who wants to improve their analysis using UV detection

Tony Edge
R&D Principal, Chromatography Consumables
Thermo Scientific

Tony Taylor
Technical Director
Crawford Scientific