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CHROMacademy Certified HPLC Courses
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HPLC Troubleshooting Guide to Cycling Baselines and Pressure Fluctuations » HPLC Mobile Phases

The Problem:
A short term cycling baseline is seen along with pressure fluctuations...
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HPLC Extra Column Volumes » HPLC Mobile Phases

It is of particular importance to reduce extra column volumes when using small volume columns or UHPLC. Where do these extra column volumes come from, how can they be minimized, and what effect do they have on chromatography?
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HPLC Mobile Phases – 10 bad habits to avoid » HPLC Mobile Phases

1) Measuring the pH of the mobile phase after the organic has been added
2) Not using a buffer
3) Not using the buffer in its correct pH range
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HPLC troubleshooting

HPLC training courses and troubleshooting from CHROMacademy

The CHROMacademy HPLC training courses cover the fundamental concepts such as the resolution equation and mechanisms of the various separation modes to more advanced topics such as gradient optimization, working principles of pumps, autosamplers and detectors.

Click Expand All to view our HPLC training courses.
Welcome to the HPLC department

Dwight R. Stoll  Assistant Professor of Chemistry  Gustavus Adolphus College  In the not-too-distant past some have suggested that HPLC as an analytical technique was mature, without much room for further development....

Dwight R. Stoll
Assistant Professor of Chemistry
Gustavus Adolphus College
www.multidlc.com/drstoll/
tutor@support.chromacademy.com

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Welcome to theCHROMacademy HPLC department

Dwight R. Stoll  Assistant Professor of Chemistry  Gustavus Adolphus College  In the not-too-distant past some have suggested that HPLC as an analytical technique was mature, without much room for further development. However, a quick review of the current literature as well as information from manufacturers of HPLC instrumentation and consumables shows us that this could not be further from the truth. Indeed, this is an exciting time to be involved with the HPLC community, with so much innovation occurring in instrumentation, stationary phase development, and methodology. Along with the excitement around this rapidly changing landscape, though, comes the challenge of 'keeping up', and CHROMacademy is the perfect vehicle to address this challenge.

In the HPLC Department we present current, dynamic, and interactive content to address the learning goals of a variety of students ranging from undergraduates to graduate students, and practicing chromatographers. I am excited to head the department, and look forward to the continued expansion of topics addressed, as well as revision of existing topics to keep them current as technology changes. I hope that you will contact me with any suggestions you have for new topics or adjustments to existing topics by writing to tutor@support.chromacademy.com.
I do hope you enjoy the site, and wish you the best in your continued learning about HPLC.

Dwight R. Stoll
Assistant Professor of Chemistry
Gustavus Adolphus College
www.multidlc.com/drstoll/