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Protein Level Analysis Introduction

As has previously been discussed, analysis at the protein level is common and provides a high level of information such as molecular weight, structural integrity, charge variants, aggregation, and modifications. It is these modifications that we are specifically focusing on when we analyze mAbs at the protein level.

Also generally included when discussing biopharmaceutical analysis at the protein level, is information pertaining to the intact protein, typically 150,000 Da for a mAb, and the large fragments. For mAb analysis these include the fragment antibody (Fab, 100,00 Da) and fragment crystallizable (Fc, 50,000 Da), produced by papain digestion, as well as the heavy chains (Hc, 50,000 Da per Hc or 100,000 Da if identical) and the light chains (25,000 Da each or 50,000 Da if identical), produced by reduction of the mAb with dithiotreitol (DTT) (Figure 1).

Intact mAb and mAb fragments

Figure 1: Intact mAb and mAb fragments [7].

 
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