The CHROMacademy Essential Guide Webcast: Troubleshooting Sample Preparation
Thursday 19th February 2015,
8:00am PDT / 11:00am EDT / 16:00 BST / 17:00 CEST
You may be familiar with the mantra “rubbish in, rubbish out”. Do not settle for putting rubbish into your analytical system, instead, take time to select the correct sample preparation technique for your sample and analytical needs, make some effort in optimizing the protocol, and look out for any problems that may arise. We will look at some of the most common sample preparation techniques and discuss how to select the appropriate one for you. We will then discuss what can go wrong during sample preparation and how you can either avoid these issues in the future or how to remedy them if they appear.
Presented by Dr. Doug Raynie (South Dakota State University) and Dr. Dawn Watson (CHROMacademy Technical Expert, Crawford Scientific).
Selecting the correct filter membrane
Avoiding unwanted analyte/membrane interactions
Matching filter dimensions to sample volume
Liquid-liquid extraction (LLE)
Acid/base/neutral extractions - where’s the analyte?
Supported liquid extraction (SLE)
The easy solution to extracting analytes from biological matrices and avoiding emulsions?
Solid phase extraction (SPE)
Dealing with low recovery
Managing problem matrices (oils, fats, high salt content, proteins etc.)
Key Learning Objectives:
Understand the different sample preparation options available and which are best suited to your sample and analytical technique
Learn how to identify when something has gone wrong with your sample preparation
Learn how to solve the most common problems encountered with sample preparation
Gain useful knowledge which will help you to get the most out of sample preparation
Who Should Attend:
All analytical scientists
Anyone who requires lower detection limits
Anyone seeing low recoveries from their chosen sample preparation technique
Find out more about this Month's Essential Guide Webcast »
Troubleshooting Sample Preparation
You may be familiar with the mantra “rubbish in, rubbish out”. Do not settle for putting rubbish into your analytical system, instead, take time to select the correct sample preparation technique for your sample and analytical needs, make some effort in optimizing the protocol, and look out for any problems that may arise.
A more detailed look at liquid-liquid extraction (LLE) and the advantages and disadvantages of this technique. The most common problem of emulsion formation will be discussed as well as the use of supported liquid extraction (SLE) as an alternative to solve this problem.
Solid phase extraction is a highly selective sample preparation technique. However, this does mean that it requires a more rigorous development protocol in comparison to more facile techniques. This module will examine each stage of the method development process as well as how to troubleshoot common problems such as low recovery or poor cleanliness.
One of the most common problems in SPE is poor or inconsistent recovery. This quick guide details how to determine if you have a recovery problem and defines step by step procedures which can be used to assess at which point in the SPE process loss of analyte(s) is occurring.
Solid phase extraction (SPE) is a routinely used technique to clean and / or concentrate samples prior to chromatography. Learn how it works and how to avoid common pitfalls to get the most out of this highly utilized sample preparation technique.
Solid phase extraction (SPE) is one of the most effective and widely used sample preparation techniques for liquid and solid samples. As analysts are asked to detect analytes at ever decreasing levels, well performed sample preparation is a must. This Essential Guide webcast will help you to better understand how SPE works, the stationary phase chemistries which are available and which analytes they are applicable too, and how to avoid common pitfalls in order to improve your SPE method.
“Before anything else, preparation is the key to success” Alexander Graham Bell. This is certainly true in analytical chemistry. The correct choice of sample preparation method for a sample prior to analysis can have a myriad of benefits; properly prepared samples will produce simpler chromatograms free from interfering peaks, instrument parts will be protected from harmful contaminants which could result in break downs and the need for increased maintenance, and the sample will be compatible with the analysis method (correct pH, solvent etc.) minimizing deleterious chromatographic effects.