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The CHROMacademy Essential Guide Webcast:
Troubleshooting Sample Preparation

Thursday 19th February 2015,
8:00am PDT / 11:00am EDT / 16:00 BST / 17:00 CEST

You may be familiar with the mantra “rubbish in, rubbish out”.  Do not settle for putting rubbish into your analytical system, instead, take time to select the correct sample preparation technique for your sample and analytical needs, make some effort in optimizing the protocol, and look out for any problems that may arise.  We will look at some of the most common sample preparation techniques and discuss how to select the appropriate one for you.  We will then discuss what can go wrong during sample preparation and how you can either avoid these issues in the future or how to remedy them if they appear.

Presented by Dr. Doug Raynie (South Dakota State University) and Dr. Dawn Watson (CHROMacademy Technical Expert, Crawford Scientific).

  • Filtration
    • Selecting the correct filter membrane
    • Avoiding unwanted analyte/membrane interactions
    • Matching filter dimensions to sample volume
  • Liquid-liquid extraction (LLE)
    • Improving quantitation
    • Treating emulsions
    • Acid/base/neutral extractions - where’s the analyte?
  • Supported liquid extraction (SLE)
    • The easy solution to extracting analytes from biological matrices and avoiding emulsions?
  • Solid phase extraction (SPE)
    • Dealing with low recovery
    • Managing problem matrices (oils, fats, high salt content, proteins etc.)

Key Learning Objectives:

  • Understand the different sample preparation options available and which are best suited to your sample and analytical technique
  • Learn how to identify when something has gone wrong with your sample preparation
  • Learn how to solve the most common problems encountered with sample preparation
  • Gain useful knowledge which will help you to get the most out of sample preparation

Who Should Attend:

  • All analytical scientists
  • Anyone who requires lower detection limits
  • Anyone seeing low recoveries from their chosen sample preparation technique
 
Troubleshooting Sample Preparation
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Troubleshooting Sample Preparation

You may be familiar with the mantra “rubbish in, rubbish out”.  Do not settle for putting rubbish into your analytical system, instead, take time to select the correct sample preparation technique for your sample and analytical needs, make some effort in optimizing the protocol, and look out for any problems that may arise. 

 

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eLearning Modules

 
Primary Sample Preparation Techniques

The basics of the most common sample preparation techniques are covered in this module. Learn which technique should be most suitable for your sample.

Sample Filtration and Ultrafiltration | Selectivity | The Partition Coefficient

 
Liquid/Liquid Extraction Techniques

A more detailed look at liquid-liquid extraction (LLE) and the advantages and disadvantages of this technique. The most common problem of emulsion formation will be discussed as well as the use of supported liquid extraction (SLE) as an alternative to solve this problem.

Liquid / liquid Extraction II | Drawbacks of Liquid/liquid Extraction | Support-Assisted Liquid / liquid Extraction II

 
Solid Phase Extraction - Method Development

Solid phase extraction is a highly selective sample preparation technique. However, this does mean that it requires a more rigorous development protocol in comparison to more facile techniques. This module will examine each stage of the method development process as well as how to troubleshoot common problems such as low recovery or poor cleanliness.

SPE Method Troubleshooting Overview | Recovery Problems II | Recovery Problems III | Reproducibility Problems II | Cleanliness Problems

 
Quick Guides
 
Investigating SPE Recovery Problems

One of the most common problems in SPE is poor or inconsistent recovery. This quick guide details how to determine if you have a recovery problem and defines step by step procedures which can be used to assess at which point in the SPE process loss of analyte(s) is occurring.

Investigating SPE Recovery Problems

 
What Can You Learn in 5 Minutes? - SPE

Solid phase extraction (SPE) is a routinely used technique to clean and / or concentrate samples prior to chromatography. Learn how it works and how to avoid common pitfalls to get the most out of this highly utilized sample preparation technique.

What Can You Learn in 5 Minutes? - SPE

 
SPE Method Development Interactive Learning Tool

We’ve provided a interactive learning tool to allow you to evaluate different sets of conditions and see what effect they have on the resulting chromatogram.

SPE Method Development Interactive Learning Tool

 
Understanding and Improving SPE

Solid phase extraction (SPE) is one of the most effective and widely used sample preparation techniques for liquid and solid samples. As analysts are asked to detect analytes at ever decreasing levels, well performed sample preparation is a must. This Essential Guide webcast will help you to better understand how SPE works, the stationary phase chemistries which are available and which analytes they are applicable too, and how to avoid common pitfalls in order to improve your SPE method.

Understanding and Improving SPE

 
Choosing the Correct Sample Preparation Technique

“Before anything else, preparation is the key to success” Alexander Graham Bell. This is certainly true in analytical chemistry. The correct choice of sample preparation method for a sample prior to analysis can have a myriad of benefits; properly prepared samples will produce simpler chromatograms free from interfering peaks, instrument parts will be protected from harmful contaminants which could result in break downs and the need for increased maintenance, and the sample will be compatible with the analysis method (correct pH, solvent etc.) minimizing deleterious chromatographic effects.

Choosing the Correct Sample Preparation Technique

 
 
 
 
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  • Understand the different sample preparation options available and which are best suited to your sample and analytical technique
  • Learn how to identify when something has gone wrong with your sample preparation
  • Learn how to solve the most common problems encountered with sample preparation
  • Gain useful knowledge which will help you to get the most out of sample preparation

Who Should Attend:

  • All analytical scientists
  • Anyone who requires lower detection limits
  • Anyone seeing low recoveries from their chosen sample preparation technique

 

Dr. Douglas Raynie
Associate Research Professor,
Department of Chemistry and Biochemistry, South Dakota State University

Dr Dawn Watson
Technical Specialist
Crawford Scientific