Techniques Employed in Biopharmaceutical Analysis - Part II Ion Exchange and SEC
Biopharmaceuticals offer great new possibilities and potential in treating various life threatening diseases such as cancer and autoimmune diseases. However, their complexity, resulting from their heterogeneity, represents a great challenge to the analytical chemist and a suite of techniques is required in the characterization and analysis.
In conjunction with RP and HILIC, covered in Part I, ion exchange chromatography (IEX) and size exclusion chromatography (SEC) are the main stay of the biopharmaceutical analyst’s arsenal. These techniques provide much more information than merely charge variance and aggregation analysis. In this event we will discuss how, why and where IEX and SEC are typically employed in biopharmaceutical characterization and quantification.
We will also highlight strategies for developing more robust IEX and SEC methods such as reducing SEC secondary interactions and contrast the use of pH and salt gradients in IEX. We will also explore the affect temperature and pressure have on SEC separations and the role of UHPLC. Finally an introduction, overview and applicability of Hydrophobic Interaction Chromatography (HIC) will also be covered.
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*NEW* Biopharmaceutical Analysis Module - Coming Soon!
in association with the Research Institute of Chromatography (Kortrijk, Belgium)
CHROMacademy are delighted to announce our forthcoming BioChromatography module covering all aspects of biopharmaceutical analysis. This major new multimedia e-learning module is being written in conjunction with the Research Institute of Chromatography (RIC), a world renowned authority on the subject. The new module will provide a thorough introduction to biopharmaceuticals and their development and will consider all aspects of chromatographic and mass spectrometric analysis of intact, modified, aggregated, charged, degraded, digested, and reduced biopharmaceuticals. The module will not only include relevant theory but will be packed with interactive movies and animations to make the learning as practically relevant as possible for those currently involved in or wishing to enter bio-pharmaceutical analysis.
The topics included will be:
- Global Pharmaceutical Market Overview
- Basics of Protein BioChemistry - Amino Acids
- Basics of Protein BioChemistry - Proteins
- Monoclonal Antibiodies (mAb) in Bipharmaceuticals
- Monoclonal Antibiodies - Form and Function
- Protein Development and Manufacture
- Biopharmaceuticals - Advances in Efficacy and Safety
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- Trastuzumab - A Model Monoclonal Antibody (mAb)
- Trastuzumab - Production and Characteristics
- Trastuzumab - Common Post Translational Modifications
- Common mAb Post Translation Modifications (PTM’s)
- Analytical Characterization at the Protein Level
- Reversed Phase HPLC Analysis of Intact and Reduced Proteins
- Chromatographic Requirements
- Chemical Derivatization
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Techniques Employed in Biopharmaceutical Analysis - Part I Reversed Phase and HILIC
Biopharmaceuticals offer great new possibilities and potential in treating various life threatening diseases such as cancer and autoimmune diseases. However, their complexity, resulting from their heterogeneity, represents a great challenge to the analytical chemist and a suite of techniques is required for their characterization and analysis. Reversed phase chromatography, the mainstay of traditional small molecule analysis, is employed at multiple biopharmaceutical levels due to its versatility, inherent relative efficiency and ease of hyphenation to a mass spectrometer.
Techniques Employed in Biopharmaceutical Analysis - Part I Reversed Phase and HILIC » |
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Introduction to Ion Chromatography
The Essential Guide from LCGC’s CHROMacademy presents the fundamental theory and instrumentation of ion chromatography.
This guide considers the underlying theory of the technique, column and eluent selection and key separation variables as well as the various techniques used for ion suppression and detection. We also consider various applications of ion chromatography together with recent advances in the technique including capillary ion chromatography, fast and ultra-high pressure ion chromatography as well as hyphenation of ion chromatography to mass spectrometric detectors.
A must see for everyone using or considering ion chromatography.
Introduction to Ion Chromatography » |
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HPLC Techniques in Biopharmaceutical Analysis
Biopharmaceuticals offer great hope in treating medical conditions which are currently poorly served at best by traditional pharmaceuticals. It is estimated that there are over 400 biopharmaceuticals in clinical trials for in excess of 200 disease areas. The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing. This Essential Guide webcast will provide details of why differing modes of chromatography are required in the research, preparation and analysis of biopharmaceuticals and when they would be typically used.
HPLC Techniques in Biopharmaceutical Analysis » |
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Characterization of Protein Biopharmaceuticals
Protein biopharmaceuticals such as monoclonal antibodies and recombinant proteins are currently in widespread use for treatment of various life-threatening diseases including cancer, autoimmune diseases, diabetes, anemia, etc. Protein therapeutics have a complexity far exceeding that of small molecule drugs, hence, unraveling this complexity represents an analytical challenge. This web seminar will highlight the power of liquid chromatography combined with mass spectrometry in the characterization of protein biopharmaceuticals.
Characterization of Protein Biopharmaceuticals » |
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The Analysis of Post Translational Modifications Using LC-MS/MS
In this session, Dr. Duncan Smith and Dr. John Griffiths (MS-Insight Ltd and Manchester University) explain the principles behind the analysis of a number of key post-translational modifications (PTMs) using LC-MS/MS techniques. They will begin with a brief overview of bottom-up proteomics, explaining how proteins are converted to peptides, which are then analyzed using electrospray LC-MS/MS. They will move on to talk about the various MS scanning techniques, which may be used for PTM analysis. Next, they will discuss in turn, how phosphorylation, acetylation, ubiquitination and SUMOylation may be analyzed using mass spectrometry, drawing upon examples from their own research at the University of Manchester. Finally, they will give their own opinions on the ways in which they see the future of PTM analysis heading.
The Analysis of Post Translational Modifications Using LC-MS/MS » |
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