Gradient Development II and Detector Settings and Optimization
In the final session we conclude our gradient optimisation work and consider several ‘real-life’ separations using computer modelling to illustrate the decision processes within gradient optimization and the possibility for optimising two parameters simultaneously. We also discuss approaches to automation in method development.
We conclude the course with a look at various detector types and how the requirements and operational performance of each detector might influence our method development choices, including the use of mass spectrometric detectors.
Amy began using chromatography at the start of her academic career with nanoflow-liquid chromatography-mass spectrometry, and a variety of other proteomics techniques, for her research into the dynamic proteome. The laboratory also focused on gas chromatographic separations of volatile biological samples.
After using chromatography for 10 years, Amy has now moved into a more training-based role with Crawford Scientific, where she hopes to share the knowledge she gained during her time in the laboratory.
Tony Taylor B.Sc. CChem FRSC Cert. Ed.
Tony has been doing, researching, teaching and training in analytical chemistry for the past 28 years. He comes from a pharmaceutical and polymer analysis background and continues to work with both liquid and gas phase techniques at Crawford Scientific (UK).
His main interests are the use of LC-MS and GC-MS for structural characterisation and the quantitation of trace components in complex matrices. He is professionally qualified trainer and is Technical Director of the CHROMacademy.
Associated Training Material from CHROMacademy
FAQs will be added as students progress through the course.
HPLC Method Development Video Training Course - Final Assessment