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The CHROMacademy Essential Guide Webcast:
Everything You Need to Know About UV Detectors for HPLC

Thursday 14th December 2017,
11:00am EST/ 8:00am PST/ 4:00pm GMT/ 5:00pm CET




Get more from your UV detector by understanding how it works.  The fundamental science behind UV detection and the working principles of this versatile detector will be discussed in detail.  Optimization strategies for key parameters will be covered in order to provide you with the tools to correctly set up your detector to provide the optimum performance for your analytical requirements.

Presented by

Dwight Stoll (Assistant Professor of Chemistry, Gustavus Adolphus College)
and Tony Taylor (Technical Director, Crawford Scientific)

Topics Covered

Practical considerations to ensure you get the most from your UV detector

  • Fundamental UV absorbance theory
  • Understanding the optical arrangements of common UV detectors
  • Data acquisition parameter optimization – bandwidth / slit settings / acquisition rates / bunch rates / spectral resolution
  • Detector linearity
  • When and how to choose a reference wavelength to reduce gradient baseline drift and noise
  • Sensitivity – lamp effects, optical and electronic noise, solvent effects etc.
  • Flow cell design
  • Detection robustness - optimizing settings based on chromatographic data and improving your analytical robustness/sensitivity

Presenter Information »


Everything You Need to Know About UV Detectors for HPLC

This month’s webcast focused on the fundamentals of UV detection for HPLC.  The CHROMacademy material provided in this month’s tutorial will expand your knowledge even further on how to get the most from your HPLC-UV methods.

Our Quick guides, CHROMacademy course content, and archived webcasts and tutorials will allow you to further advance your knowledge of HPLC detectors as well as provide essential know how on selecting, optimizing, and troubleshooting detectors.

Watch the webcast

eLearning Modules


This module will help you understand the working principles of the most commonly used HPLC detectors. By understanding how these detectors work you will be able to select and optimize the best detector for your method.

General Terms and Concepts | Limit of Detection / Quantification
UV - Vis Detectors, The Flow Cell | Variable Wavelength UV - Vis Detectors | Diode Array Detectors

Quick Guides

5 Diode Array Detector Settings that Should be Optimized

Don’t just set and forget your detector parameters. Let us help you make the most of your diode array detector with our guide to the five settings that should be optimized and our quick optimization tips.

Diode Array Detector Settings »


HPLC Gradient Elution - Baseline Drift

What causes baseline drift in HPLC-UV gradient methods and how it can be solved or avoided?

Baseline Drift »


10 Good Reasons for Adding a Mass Spectrometer to my UV-HPLC

Take you HPLC-UV methods to the next level by considering also using a mass spectrometer.

Adding a Mass Spectrometer to my UV-HPLC »

HPLC Detectors - What, Where, When, and How

The selection of a detector for HPLC is based on the chemical nature of the analytes and any potential interferents that may be present, the limit of detection required, and often the availability and cost of the detector. This month’s Essential Guide will focus on the operating principles of the most common HPLC detectors. The parameters that should be optimized for each detector type will be examined to give the optimum performance from the chosen detector. The advantages, disadvantages, and application areas for each detector type will also be discussed. This in depth review of HPLC detectors will result in a better understanding of the operation, optimization, and detector choice for every application.

HPLC Detectors »

What HPLC Operators Need to Know: Part II - Everything Needed to Understand Column Selection Mobile Phase Gradients, and Detectors

Column selection probably one of the most important choices in any chromatographic method. We will share our insights on how to select the correct column each time covering particle size, morphology, physicochemical properties as well as stationary phase selection. We will also detail how to utilize and optimize HPLC gradients and review the various detectors available and when and how they are best employed.

Column Selection Mobile Phase Gradients, and Detectors »

HPLC Troubleshooting - Autosampler, Column, and Detector Issues

Practical troubleshooting and maintenance information associated with HPLC autosamplers, columns and detectors. We will build a checklist of suggested maintenance operations as well as outlining common diagnostic tests.

Autosampler, Column, and Detector Issues »

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Dwight Stoll
Assistant Professor of Chemistry,
Gustavus Adolphus College


Tony Taylor
Technical Director, Crawford Scientific

Tony comes from a pharmaceutical background and has many years’ research and development experience in small molecule analysis and bio-analysis using LC, GC and hyphenated MS techniques.
He is actively involved in method development within the analytical services laboratory at Hall Analytical – which supplies contract research in Chromatography and Mass Spectrometry. He continues to research in novel separation technologies, chromatographic method development and structural characterisation, especially in the areas of Extractable and Leachable analysis and Bio-Chromatography.

Tony is the Technical Director of CHROMacademy and has spent the past 17 years as a trainer and developing on-line education materials in analytical chemistry techniques.