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The CHROMacademy Essential Guide Webcast:
Hydrophobic Interaction Chromatography of mAbs and ADCs – Not Just Another Way of Performing Reversed Phase-Like Separations

North America: Thursday 21st June 2018,
11:00am EDT/ 10:00am CDT/ 8:00am PDT

Europe: Friday 22nd June 2018,
9:00am BST/ 10:00am CEST

Asia Pacific: Friday 22nd June 2018,
8:00am IST/ 10:30am CST/ 11:30am JST

 

sponsored by Agilent

Hydrophobic interaction chromatography, or HIC, is a very powerful chromatographic technique for separating proteins in order of increasing hydrophobicity.  Unlike traditional reversed phase chromatography, it is conducted using mobile phase solutions that do not denature the protein.  It is also capable of resolving impurities and variants (from post-translational modifications) that can prove difficult by any other chromatographic technique.

Learn more about the important factors that need to be considered in developing robust and reliable UHPLC-based HIC separations and how novel HIC stationary phases can solve current industry challenges in determining critical quality attributes of mAbs and ADCs.

Presented by

Presented by Dr. Andrew Coffey (Senior Application Chemist, Agilent Technologies).

Presenter Information »

 
 
The CHROMacademy Essential Guide Tutorial:
Hydrophobic Interaction Chromatography of mAbs and ADCs
  Sponsored by Agilent

This month’s webcast discussed the use of hydrophobic interaction chromatography for the analysis of mAbs and ADCs.  The CHROMacademy material provided in this month’s tutorial will give you more knowledge of analyzing mAbs and ADCs using a variety of analytical techniques.

Our Quick guides, CHROMacademy course content, and archived webcasts and tutorials will provide you with a well-rounded understanding of the techniques used to analyze biopharmaceuticals.

Watch the webcast
eLearning Modules

Reversed Phase - Protein Level Analysis and Optimization - FREE MODULE

This module will help you to understand how to apply reversed phase HPLC to the analysis of proteins.  The content includes information on starting method conditions and how parameters such as flow rate, gradient, and organic modifier can impact the separations.

Reversed Phase – Protein Level Analysis and Optimization »

 

Mass Spectrometry at the Protein Level - FREE MODULE

This module will consider how mass spectrometry is applied to biomolecule analysis at the protein level. The topics covered include determining molecular weight and coupling the common HPLC techniques which are used for analyzing intact proteins with mass spectrometry.

Mass Spectrometry at the Protein Level »

 

Quick Guides


Hydrophobic Interaction Chromatography for Biopharmaceutical Analysis

Hydrophobic interaction chromatography (HIC) is a milder form of reversed phase liquid chromatography (RPLC).  Separation of analytes is based on hydrophobic interactions with the stationary phase; therefore, the elution order in HIC enables proteins to be ranked on the basis of their relative hydrophobicity.  HIC employs non-denaturing conditions, does not require the use of organic solvents or high temperatures, and separations are carried out at physiological pH allowing the preservation of protein structure.

Hydrophobic Interaction Chromatography for Biopharmaceutical Analysis »

 

Reversed Phase HPLC for the Analysis of Biomolecules

Commonly reversed phase (RP), hydrophilic interaction (HILIC), ion exchange (IEX), and size exclusion (SEC) chromatographic modes will be combined to allow full characterization of complex biomolecules. The following article will introduce the fundamentals of biopharmaceutical analysis and cover the use of reversed phase HPLC in their analysis.

Reversed Phase HPLC for the Analysis of Biomolecules »

 

HILIC, IEX, and SEC for the Analysis of Biomolecules

The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing. This article will examine the use of HILIC, IEX, and SEC for their full characterization.

HILIC, IEX, and SEC for the Analysis of Biomolecules »

 

Techniques Employed in Biopharmaceutical Analysis – Part II Ion Exchange and SEC

Biopharmaceuticals offer great new possibilities and potential in treating various life-threatening diseases such as cancer and autoimmune diseases. However, their complexity, resulting from their heterogeneity, represents a great challenge to the analytical chemist and a suite of techniques is required in the characterization and analysis. In conjunction with RP and HILIC, covered in Part I, ion exchange chromatography (IEX) and size exclusion chromatography (SEC) are the main stay of the biopharmaceutical analyst’s arsenal. These techniques provide much more information than merely charge variance and aggregation analysis. In this event we will discuss how, why and where IEX and SEC are typically employed in biopharmaceutical characterization and quantification. We will also highlight strategies for developing more robust IEX and SEC methods such as reducing SEC secondary interactions and contrast the use of pH and salt gradients in IEX. We will also explore the affect temperature and pressure have on SEC separations and the role of UHPLC.

Techniques Employed in Biopharmaceutical Analysis – Part II Ion Exchange and SEC »

 

HPLC Techniques in Biopharmaceutical Analysis

Biopharmaceuticals offer great hope in treating medical conditions which are currently poorly served at best by traditional pharmaceuticals.  It is estimated that there are over 400 biopharmaceuticals in clinical trials for in excess of 200 disease areas.  The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing.  This Essential Guide webcast will provide details of why differing modes of chromatography are required in the research, preparation and analysis of biopharmaceuticals and when they would be typically used.

HPLC Techniques in Biopharmaceutical Analysis »

 

Characterization of Protein Biopharmaceuticals

Protein biopharmaceuticals such as monoclonal antibodies and recombinant proteins are currently in widespread use for treatment of various life-threatening diseases including cancer, autoimmune diseases, diabetes, anemia, etc. Protein therapeutics have a complexity far exceeding that of small molecule drugs, hence, unraveling this complexity represents an analytical challenge. This web seminar will highlight the power of liquid chromatography combined with mass spectrometry in the characterization of protein biopharmaceuticals.

Characterization of Protein Biopharmaceuticals »

 
 
 
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Dr. Andrew Coffey
Senior Applications Chemist
Agilent Technologies

 

Andy joined Polymer Laboratories in 1989 after completing his PhD in peptide synthesis at Wolverhampton, UK.

He progressed from Senior Scientist to Product Manager looking after multiple product lines.  He continued as Product Manager during the acquisition by Varian and then Agilent Technologies, and joined the Biocolumns Applications Team in August 2011. 

Andy has considerable experience in reversed phase, ion-exchange and size exclusion liquid chromatography method development, technical support, and troubleshooting.