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The CHROMacademy Essential Guide Webcast:
Top Tips to Improve Reproducibility and Sensitivity in Solid Phase Extraction

Thursday 22nd March 2018,
8:00am PDT/ 11:00am EDT/ 3:00pm GMT/ 4:00pm CET


 

 

 

A robust and sensitive method is the product of meticulous method development. In this webcast we will consider the most pertinent steps in SPE method development and how the decisions which are made can impact on reproducibility and sensitivity.

Presented by

Dr. Doug Raynie (Associate Research Professor, Department of Chemistry and Biochemistry, South Dakota State University)
Tony Taylor (Technical Director, Crawford Scientific)

Topics Covered

Essential SPE method optimization

  • Sorbent selection – the key to good reproducibility and sensitivity
  • Understanding your extraction chemistry
  • Tips for optimizing SPE protocol steps
  • Sorbent Equilibration and Sample loading considerations
  • Solvent flows, soak steps and drying steps – expert tips
  • Wash steps - how to avoid the hazards and optimize your recovery
  • Sample elution – be brave to achieve lower detection limits
  • Matrix effects and how to overcome them

Presenter Information »

 
 

Top Tips to Improve Reproducibility and Sensitivity in Solid Phase Extraction.

This month’s webcast provided you with our top tips for improving SPE reproducibility and sensitivity.  The CHROMacademy material provided in this month’s tutorial will give you further insight into the fundamentals of SPE as well as the process of method development.

Our Quick guides, CHROMacademy course content, and archived webcasts and tutorials will provide you with an excellent scientific knowledge of SPE fundamentals, method development, and troubleshooting.

Watch the webcast

eLearning Modules


 

SPE Overview

The perfect quick guide to solid phase extraction (SPE).  Understand the essentials of SPE including, the terminology, sorbent characteristics, and protocol steps.

SPE Terminology | SPE Sorbent Substrates
Choosing Sorbent Mass | Protocol Steps in SPE
 

SPE Method Development

A comprehensive step-by-step guide to developing SPE methods.

Sorbent Bed Geometry | SPE Method Development
Analyte Assessment | Mechanism Selection
 

Quick Guides


Analyte Chemistry for SPE

The first step in developing a solid phase extraction (SPE) method is understanding your analyte(s) chemistry.  Analyte chemistry dictates physical parameters such as solubility.  It also dictates the interaction of the analyte with both the sorbent and solvents used in the SPE method, as well as any matrix components (e.g. proteins) which could interfere with the extraction method.

Analyte Chemistry for SPE »

 

SPE Mechanism Selection

Selecting the optimum SPE mechanism based on your analyte(s) and matrix is essential for providing clean extracts.  This Quick Guide will look at the process of selecting the correct extraction mechanism.

SPE Mechanism Selection »

 

Troubleshooting SPE Reproducibility Problems

Problems with reproducibility is one of the most common issues in SPE.  This Quick Guide will look at how to diagnose reproducibility problems and how to solve them.

Troubleshooting SPE Reproducibility Problems »

 

Investigating SPE Recovery Problems

One of the most common problems in SPE is poor or inconsistent recovery.  This quick guide details how to determine if you have a recovery problem and defines step by step procedures which can be used to assess at which point in the SPE process loss of analyte(s) is occurring.

Investigating SPE Recovery Problems »

 

Choosing the Correct Sample Preparation Technique

“Before anything else, preparation is the key to success” Alexander Graham Bell. This is certainly true in analytical chemistry. The correct choice of sample preparation method for a sample prior to analysis can have a myriad of benefits; properly prepared samples will produce simpler chromatograms free from interfering peaks, instrument parts will be protected from harmful contaminants which could result in break downs and the need for increased maintenance, and the sample will be compatible with the analysis method (correct pH, solvent etc.) minimizing deleterious chromatographic effects. This Essential Guide webcast will provide details of sample preparation techniques and guide you to the correct method for your sample; whether it is a simple liquid/liquid extraction or a more complex solid phase extraction.

Choosing the Correct Sample Preparation Technique »

 

Troubleshooting Sample Preparation

You may be familiar with the mantra “rubbish in, rubbish out”.  Do not settle for putting rubbish into your analytical system, instead, take time to select the correct sample preparation technique for your sample and analytical needs, make some effort in optimizing the protocol, and look out for any problems that may arise.  We will look at some of the most common sample preparation techniques and discuss how to select the appropriate one for you.  We will then discuss what can go wrong during sample preparation and how you can either avoid these issues in the future or how to remedy them if they appear.

Troubleshooting Sample Preparation »

 
 
 
 
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Dr. Doug Raynie
Associate Research Professor,
Department of Chemistry and Biochemistry,
South Dakota State University

Dr. Doug Raynie is an Associate Research Professor in the Department of Chemistry and Biochemistry at South Dakota State University.  Prior to joining SDSU, he was employed for eleven years as a Senior Scientist at Procter and Gamble's Corporate Research Division.  He earned his Ph.D. at Brigham Young University under the direction of Dr. Milton L. Lee.  His undergraduate degree is from Augustana (South Dakota) College, with majors in chemistry and biology.

Dr. Raynie’s broad research interests are in the field of sustainability and green chemistry.  His two major areas of research are bioprocessing using supercritical fluids and related technologies and analytical separations.  Active projects in this area include transformations of lignocellulosic biomass using supercritical fluids and ionic liquids.  Analytical separations research includes utilization of deep eutectic solvents, high-resolution chromatography (high-temperature LC and SFC), chromatographic sample preparation (ASE, SFE, SPME, and SPE), chromatography theory, green analytical chemistry, and problem-based learning in analytical chemistry. Dr. Raynie serves on the editorial advisory boards of the Encyclopedia of Separation Science and Pharmaceutical Formulation and Quality


Tony Taylor
Technical Director, Crawford Scientific


Tony comes from a pharmaceutical background and has many years’ research and development experience in small molecule analysis and bio-analysis using LC, GC and hyphenated MS techniques.

Tony is actively involved in method development within the analytical services laboratory at Hall Analytical – which supplies contract research in Chromatography and Mass Spectrometry. He continues to research in novel separation technologies, chromatographic method development and structural characterisation, especially in the areas of Extractable and Leachable analysis and Bio-Chromatography.

Tony is the Technical Director of CHROMacademy and has spent the past 17 years as a trainer and developing on-line education materials in analytical chemistry techniques.