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The CHROMacademy Essential Guide Webcast:
Choosing the Right Column for Intact Protein Characterization – a Deeper Dive into Column Characteristics

North America: Thursday 24th May 2018,
11:00am EDT/ 10:00am CDT/ 8:00am PDT

Europe: Friday 25th May 2018,
9:00am BST/ 10:00am CEST

Asia Pacific: Friday 25th May 2018,
8:00am IST/ 10:30am CST/ 11:30am JST

 

Agilent

 

This webcast will investigate some of the parameters you need to take into consideration when choosing the most appropriate chromatography column to get the best out of your instrumentation and provide the optimum resolution for your protein characterization.

Presented by

Presented by Dr. Andrew Coffey (Senior Application Chemist, Agilent Technologies).

Topics Covered

  • Effects of particle size, pore size, and bonding chemistry on column performance
  • Attributes to ensure optimum separations for protein characterization

Presenter Information »

 
 

Choosing the Right Column for Intact Protein Characterization Tutorial.

This month’s webcast took a closer look at the column requirements for protein analysis.  The CHROMacademy material provided in this month’s tutorial will provide you with further knowledge on the techniques used for intact protein characterization and the information which can be gained.

Our Quick guides, CHROMacademy course content, and archived webcasts and tutorials will provide you with an excellent scientific knowledge of the application of HPLC to intact protein separations.

Watch the webcast
eLearning Modules

Reversed Phase – Protein Level Analysis and Optimization

This module will help you to understand how to apply reversed phase HPLC to the analysis of proteins.  The content includes information on starting method conditions and how parameters such as flow rate, gradient, and organic modifier can impact the separations.

Reversed Phase – Protein Level Analysis and Optimization »
 

Quick Guides


Reversed Phase HPLC for the Analysis of Biomolecules

Commonly reversed phase (RP), hydrophilic interaction (HILIC), ion exchange (IEX), and size exclusion (SEC) chromatographic modes will be combined to allow full characterization of complex biomolecules. The following article will introduce the fundamentals of biopharmaceutical analysis and cover the use of reversed phase HPLC in their analysis.

Reversed Phase HPLC for the Analysis of Biomolecules »

 

HILIC, IEX, and SEC for the Analysis of Biomolecules

The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing. This article will examine the use of HILIC, IEX, and SEC for their full characterization.

HILIC, IEX, and SEC for the Analysis of Biomolecules »

 

Hydrophobic Interaction Chromatography for Biopharmaceutical Analysis

Hydrophobic interaction chromatography is a useful tool for analyzing biopharmaceuticals as it utilizes non-denaturing conditions allowing for the preservation of protein structure.  This technique has been widely applied at all stages of the purification process as well for the analysis of antibody drug conjugates (ADCs).

Hydrophobic Interaction Chromatography for Biopharmaceutical Analysis »

 

Choosing the Correct Column & Mobile Phase for LC-MS Characterization of Biologics

This webcast will focus on the parameters which are essential for the development of a successful RPLC-MS method for the characterization of a monoclonal antibody.

 

Choosing the Correct Column & Mobile Phase for LC-MS Characterization of Biologics »

 

Techniques Employed in Biopharmaceutical Analysis - Part I Reversed Phase and HILIC

This webcast shall interrogate the critical parameters required to successfully implement and optimize reversed phase analysis of biopharmaceuticals. We will also discuss the popularity behind glycosylated biopharmaceuticals, where heterogeneity arises in these molecules, and how glycans can be effectively liberated, characterized, and analyzed using HILIC.

Techniques Employed in Biopharmaceutical Analysis - Part I Reversed Phase and HILIC »

Characterization of Protein Biopharmaceuticals

Protein biopharmaceuticals, such as monoclonal antibodies and recombinant proteins, are currently in widespread use for treatment of various life-threatening diseases including cancer, autoimmune diseases, diabetes, anemia, etc. Protein therapeutics have a complexity far exceeding that of small molecule drugs, hence, unraveling this complexity represents an analytical challenge. This webcast will highlight the power of liquid chromatography combined with mass spectrometry in the characterization of protein biopharmaceuticals.

Characterization of Protein Biopharmaceuticals »

 
 
 
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Dr. Andrew Coffey
Senior Applications Chemist
Agilent Technologies

 

Andy joined Polymer Laboratories in 1989 after completing his PhD in peptide synthesis at Wolverhampton, UK.

He progressed from Senior Scientist to Product Manager looking after multiple product lines.  He continued as Product Manager during the acquisition by Varian and then Agilent Technologies, and joined the Biocolumns Applications Team in August 2011. 

Andy has considerable experience in reversed phase, ion-exchange and size exclusion liquid chromatography method development, technical support, and troubleshooting.