No thanks! I would like to know more about CHROMacademy

 Over 3000 E-Learning topics / 5000 Articles & Applications

The CHROMacademy Essential Guide Webcast:
Optimize and Troubleshoot Your HPLC System:
Degassers, Pumps, and Autosamplers

Thursday 22nd June 2017,
8:00am PST/ 11:00am EST/ 4:00pm BST/ 4:00pm CET


Learn how HPLC instrument components function in order to correctly maintain and troubleshoot the system for maximum up-time and optimum chromatography.  This webcast will focus on the degasser, pumps, and autosampler. 

Poorly degassed solvents will cause noisy baselines and columns may be damaged; pumps which do not deliver the correct flow rate can result in drifting retention times; while an autosampler which does not deliver the correct sample volume will impact peak shape and quantitation.   

Presented by

Tony Taylor (Technical Director, Crawford Scientific)

Topics Covered

Get the most out of your instrument by understanding the fundamentals of solvent delivery and sample introduction.

  • Basic principles of degasser design
  • Binary and Quaternary pump design, operation and optimization
  • Operating principles of common autosampler designs
  • Principles of the injection valve
  • Troubleshooting problems and proper maintenance of HPLC degassers, pumps and autosamplers

Presenter Information »


Optimize and Troubleshoot Your HPLC System: Degassers, Pumps, and Autosamplers - Tutorial

This month’s webcast focused on the working principles of HPLC degassers, pumps, and autosamplers.  Our tutorial material has been selected to further your knowledge of these system components; how they function, how to maintain them, and how to troubleshoot them when something goes wrong.

Our Quick guides, CHROMacademy course content, and archived webcasts and tutorials will provide the information required to get the most out of your HPLC by understanding how it works.

Watch the webcast

eLearning Modules

Mobile Phase Considerations

The chemical nature of the mobile phase can be altered to affect the retention and separation of analyte molecules.  However, there are some physical parameters which must be considered such as, viscosity, UV cut off, and mobile phase preparation (degassing, filtering etc.) in order to obtain successful analyses.

Degassing | Outlet Degassing | Degassing / Column Damage | Methods of Degassing

HPLC Solvent Pumping Systems

The HPLC pump is the heart of the chromatographic system.  It is important to understand how the pump works and what to look for if something in the pumping system fails.  With a plethora of interactive content this CHROMacademy elearning module will be invaluable in helping all users understand the inner workings of an HPLC pumping system.  In relation to gradient HPLC, the step by step guide to determining dwell volume is a must read.

Binary Pumps | Quaternary Pumps | High Back Pressure | Check Valves and Pistons


The common autosampler designs are elegantly described using our CHROMacademy animations.  This module will provide you with the knowledge of how an autosampler works; this will have many benefits including better method development, faster troubleshooting, and improved system care and maintenance.

Injection Valve Anatomy | Pull to Fill Auto Samplers | Push to Fill Auto Samplers | Integral Loop Auto Samplers


Quick Guides

Autosampler Contamination

Contamination in any chromatographic method is a complex problem to troubleshoot.  Extra peaks, often referred to as ghost peaks, in a blank or sample chromatogram can have a number of sources.

Autosampler Contamination »

HPLC Troubleshooting Guide to Cycling Baselines and Pressure Fluctuations

A quick guide to using the CHROMacademy HPLC troubleshooter to solve a baseline problem.

Cycling Baselines and Pressure Fluctuations »

Chromatography Troubleshooting Tips

Our top 10 chromatography troubleshooting tips.

10 Chromatography Troubleshooting Tips »

HPLC Troubleshooting - Autosampler, Column, & Detector

Practical troubleshooting and maintenance information associated with HPLC autosamplers, columns and detectors.  This session examines different autosampler designs, injection valve operations, and common problems associated with modern HPLC autosamplers.  Sample solvent and injection volume effects on chromatography will also be discussed in detail.  We will consider column hardware design, materials of construction and associated issues – including minimizing dead volume and avoiding/troubleshooting column blockage issues, especially with the popular sub-2 µm column packing materials.  We will also investigate the importance of controlling the temperature of the column during analysis and the potential chromatographic effects of poor temperature control.  We will conclude by reviewing common HPLC detector hardware problems and the chromatographic issues associated with detector hardware and acquisition settings.   We will build a checklist of suggested maintenance operations as well as outlining common diagnostic tests.

Troubleshooting: Autosampler, Column, & Detector »

HPLC Troubleshooting - Eluents and Solvent Delivery Systems

Practical troubleshooting and maintenance information around HPLC eluents and solvent delivery systems (pumps).  This session considers important practical concepts such as good practice for eluent preparation, filtration, and degassing as well as common related issues such as retention time drift and selectivity changes.  We also explain the working principles of HPLC solvent delivery systems and highlight the components which are most susceptible to problems.  The webcast concludes by considering various troubleshooting tests and strategies, as well as common maintenance operations for solvent delivery systems. A must see for anyone who uses HPLC equipment and who wants to improve their troubleshooting and instrument maintenance skills.

Troubleshooting: Eluents and Solvent Delivery Systems »

HPLC Troubleshooting Masterclass

Using real world chromatograms submitted by CHROMacademy members from many different application areas, this webcast illustrates how to recognize problems and explains further tests and investigations necessary to properly qualify the issue.  They then offer advice on how the problem may be resolved to help you gain valuable insight into recognizing, characterizing, and fixing problems in HPLC to improve your data quality and reduce instrument downtime.

HPLC Troubleshooting Masterclass »

loading data
loading data
loading data
loading data
loading data
Home | About UsContact Us | SubscribeTerms and Conditions | Advertise | Privacy Policy |

loading data

loading data

loading data


loading data

loading data


Tony Taylor
Technical Director
Crawford Scientific

Tony comes from a pharmaceutical background and has many years’ research and development experience in small molecule analysis and bio-analysis using LC, GC and hyphenated MS techniques.

Tony is actively involved in method development within the analytical services laboratory at Hall Analytical – which supplies contract research in Chromatography and Mass Spectrometry. He continues to research in novel separation technologies, chromatographic method development and structural characterization, especially in the areas of Extractable and Leachable analysis and Bio-Chromatography.

Tony is the Technical Director of the CHROMacademy and has spent the past 17 years as a trainer and developing on-line education materials in analytical chemistry techniques.