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Ion exchange chromatography (IEX) is based on the different affinities of analyte ions for oppositely charged ionic functional groups (ligands) bound to the stationary phase surface, and the ability of these analyte ions to displace counter ions associated with the stationary phase ligands. This module discusses ion exchange techniques and applications for the analysis of biomolecules.

 

Topics include:

  • Ion exchange chormatography
  • Ion exchange mechanisms
  • Ion exchange elution considerations
  • Ion exchange chromatographic process
  • Ion exchange chromatography for the analysis of biomolecules
  • Salt gradient elution conditions
  • pH gradient elution conditions
  • Column selection for biomolecule analysis by IEX
  • Ion exchange chromatography and MS detection
  • Method development and selection of initial conditions