check
Success: Your bookmark has been successfully created.
check
Success: Your bookmark has been successfully removed.
check
Success: Password updated and now signed in
error_outline
Success: Your password has been updated

Available to following accounts
Academic
Premier
Accredited by

 

 

This webcast will focus on the parameters which are essential for the development of a successful RPLC-MS method for the characterization of a monoclonal antibody. The methods will allow for the analysis of the intact species, determination of absolute mass, examination of the major fragments, (Fab/Fc and Hc/Lc), and investigation of the small peptides produced by protease digestion. The importance of pore size and mobile phase additives will be addressed and guidance given on how to optimize methods for sensitivity and resolution.

 

Topics include:

  • Choosing the correct column pore size for mAb intact, fragment, and peptide analysis
  • Impact of column chemistry on selectivity and resolution
  • Impact of mobile phases additives on selectivity and sensitivity