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This webcast will focus on understanding the complexity of biotherapeutic protein charge variant analysis and how optimization of weak cation exchange chromatography is a necessity. Development of robust experimental method conditions can only be found by a systematic screening approach, since each protein will retained differently depending on its sequence and quaternary structure. Coupling the optimized method to a mass spectrometry detector can be achieved by using offline or online (2D) desalting techniques to enable each peak to be fully characterized.

 

Topics include:

  • Charge variants and how they arise
  • Use of cation exchange chromatography to analyze native protein charge variants
  • Use of software to dynamically create changing buffer conditions for method optimization
  • Transferring to MS-compatible mobile phase for greater understanding of individual charge variants