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This webcast presents a comprehensive review and tutorial on hydrophilic interaction chromatography (HILIC). This technique is very useful for retention and separation of polar and ionizable compounds and provides a very useful orthogonal separation mechanism for chromatographers. We include a comprehensive review of the mechanisms of HILIC separations as well as considering the major parameters which affect the separation selectivity including eluent solvent composition, pH, and buffer type and strength.

There are a host of practical tips and tricks to ensuring a successful separation as well as a guide to stationary phases for HILIC separations. A must for everyone using, or considering the use of HILIC to improve the separation of polar analytes.