HPLC Column Issues

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HPLC Column Issues

The column is at the heart of the HPLC separation, therefore, column related issues can have a big impact on the reliability of our method. We will consider the most common sources of common related issues, allowing you to troubleshoot problematic methods as well as avoiding many of these pitfalls in the first place.

Topics include:

The HPLC column
Effect of physical parameters
- Length
- Internal diameter
- Particle size
- Solid silica support
Bonded phases
Endcapping
Chemical limitations
Column comparison
Batch variability
Column damage and associated symptoms
Column protection and repair

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