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Sample Preparation - Supported Liquid Extraction (SLE)

An interesting and useful variation of liquid-liquid extraction (LLE) is supported liquid extraction (SLE).  Compounds are separated on the same basis of differential solubility, as with conventional liquid-liquid extraction.  While the chemistry of this technique is very similar to that of LLE the physical nature of the technique offers distinct benefits; such as the ability to automate due to the use of 96 well plate or column format tubes, cost effectiveness due to low solvent consumption, alleviation of emulsions which can be formed during traditional liquid/liquid extraction protocols, and improved recoveries and reproducibility in comparison to conventional liquid/liquid extraction.

 

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Dr. Dawn Watson
 

This article was written by Dr. Dawn Watson.

Dawn received her PhD in synthetic inorganic chemistry from the University of Strathclyde, Glasgow. The focus of her PhD thesis was the synthesis and application of soft scorpionate ligands. As well as synthetic skills, this work relied on the use of a wide variety of analytical techniques, such as, NMR, mass spectrometry (MS), Raman spectroscopy, infrared spectroscopy (IR), UV-visible spectroscopy, electrochemistry, and thermogravimetric analysis.

Following her PhD she spent two years as a postdoctoral research fellow at Princeton University studying the reaction kinetics of small molecule oxidation by catalysts based on Cytochrome P450. In order to monitor these reactions stopped-flow kinetics, NMR, HPLC, GC-MS, and LC-MS techniques were utilized.

Prior to joining the Crawford Scientific and CHROMacademy technical team she worked for Gilson providing sales and support for the entire product range including, HPLC (both analytical and preparative), solid phase extraction, automated liquid handling, mass spec, pipettes, and laboratory consumables.

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