- I get molecular weight information on my peak. An MS gives you the mass to charge ratio of any ions it sees, which is a hugely useful piece of information.
RT and area aren’t enough for identification purposes, but once you add weight information to the equation, things start to become more certain.
- Better selectivity – a complex chromatogram can be like trying to see through a forest when you use a UV detector – it just sees trees.
We can set the MS to look at the forest and only show trees of an exact height (e.g. 30.8ft), making things much clearer and more visible
- Better sensitivity – generally mass spectrometry (MS)is inherently more sensitive in terms of analyte response, but sensitivity is a feature of signal to noise.
- Are you seeing the full picture? If using a single wavelength UV detector, we rely on everything having the exact chromaphore that absorbs that wavelength.
If the chromaphore is different between the compounds in our chromatogram, we may not be seeing compounds that are present.
- You can see what’s under you main peak. If you’ve developed a method, there’s always the risk that something small is lurking under your main peak(s).
With MS you can detect compounds at levels of 0.1% that fully co-elute with the main peak.