In this session we will consider how our sample analyte molecules are transferred through the injection system and onto the column.
This will cover important considerations for operation and optimisation of split and splitless injections, as well as a look at more specialised injection techniques for thermally unstable samples, such as cool-on-column and PTV inlets.
The session will conclude with a discussion of band broadening and how column dimensions and choice of carrier gas will have a direct effect on the efficiency of your separations
Amy began using chromatography at the start of her academic career with nanoflow-liquid chromatography-mass spectrometry, and a variety of other proteomics techniques, for her research into the dynamic proteome. The laboratory also focused on gas chromatographic separations of volatile biological samples.
After using chromatography for 10 years, Amy has now moved into a more training-based role with Crawford Scientific, where she hopes to share the knowledge she gained during her time in the laboratory.
Tony Taylor B.Sc. CChem FRSC Cert. Ed.
Tony has been doing, researching, teaching and training in analytical chemistry for the past 28 years. He comes from a pharmaceutical and polymer analysis background and continues to work with both liquid and gas phase techniques at Crawford Scientific (UK).
His main interests are the use of LC-MS and GC-MS for structural characterisation and the quantitation of trace components in complex matrices. He is professionally qualified trainer and is Technical Director of the CHROMacademy.
Associated Training Material from CHROMacademy
FAQs will be added as students progress through the course.