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Fundamentals of LC-MS - Video Training Course
Session 3

HPLC Considerations, Detectors and Vacuum systems
Duration 1:54hr

Session 3 returns to the HPLC side of this analysis method, with detailed discussions on the effects that various aspects of the LC separation may have on your MS data.

We will include effects of solvent types and the common LC additives, for both ESI and APCI ionization – looking at their effect on ionization efficiency, desolvation and adduct formation.

In the second part of this session we take a look at other important components of typical mass spectrometers.

We will briefly describe how the detector itself works, illustrating the importance of controlling how ions travel through the mass spectrometer. We will then discuss the vacuum system and the importance of this. Some general maintenance hints and tips will be included.

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Tutor support

Amy Claydon BSc PhD

Amy began using chromatography at the start of her academic career with nanoflow-liquid chromatography-mass spectrometry, and a variety of other proteomics techniques, for her research into the dynamic proteome. The laboratory also focused on gas chromatographic separations of volatile biological samples.
After using chromatography for 10 years, Amy has now moved into a more training-based role with Crawford Scientific, where she hopes to share the knowledge she gained during her time in the laboratory.

  Amy Claydon

Tony Taylor B.Sc. CChem FRSC Cert. Ed.

Tony has been doing, researching, teaching and training in analytical chemistry for the past 28 years. He comes from a pharmaceutical and polymer analysis background and continues to work with both liquid and gas phase techniques at Crawford Scientific (UK).
His main interests are the use of LC-MS and GC-MS for structural characterisation and the quantitation of trace components in complex matrices. He is professionally qualified trainer and is Technical Director of the CHROMacademy.

  Tony Taylor

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