Data Interpretation and Introduction to method Development
In session 4 we will concentrate on the data produced during typical LC-MS analyses and give an overview of data interpretation and structural elucidation from MSMS data.
The course concludes with a summary of the many parameters and considerations that require optimization to obtain the best possible data from your LC-MS analyses. Using the information from the earlier sessions we will assess factors such as optimizing the sprayer position and voltages, as well as the effects of eluent flow rates and pneumatic assistance.
The session concludes with a summary of key guidelines to achieve the best LC-MS data with your samples.
Amy began using chromatography at the start of her academic career with nanoflow-liquid chromatography-mass spectrometry, and a variety of other proteomics techniques, for her research into the dynamic proteome. The laboratory also focused on gas chromatographic separations of volatile biological samples.
After using chromatography for 10 years, Amy has now moved into a more training-based role with Crawford Scientific, where she hopes to share the knowledge she gained during her time in the laboratory.
Tony Taylor B.Sc. CChem FRSC Cert. Ed.
Tony has been doing, researching, teaching and training in analytical chemistry for the past 28 years. He comes from a pharmaceutical and polymer analysis background and continues to work with both liquid and gas phase techniques at Crawford Scientific (UK).
His main interests are the use of LC-MS and GC-MS for structural characterisation and the quantitation of trace components in complex matrices. He is professionally qualified trainer and is Technical Director of the CHROMacademy.
Associated Training Material from CHROMacademy
FAQs will be added as students progress through the course.
Fundamentals of LC-MS Video Course - Final Assessment