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High performance liquid chromatography with optical detection or coupled to mass spectrometry are fundamental methods for characterization and quality control of biopharmaceutical products. Biotherapeutic molecules represent a versatile class of compounds that need special attention for sample preparation, LC instrumentation, and type of chromatography columns used. This webcast will showcase the challenges analytical laboratories face when analyzing biotherapeutics and how the hurdles of compromised sample integrity and surface interaction may be overcome.