The Atmospheric Pressure Interface (API) is the core element to the course with the principles of operation, limitations and applicability fully explored. The course covers ion suppression, the use of Electrospray or APC and MS-MS data acquisition modes.

Optimization of interface and mass filter settings and how to best utilize reduced dimension LC to speed up sample throughput will be discussed. All popular interface types and mass analyzing equipment (Quadrupole, TOF, Ion Trap etc.) will be comprehensively covered.

Course Delivery & Duration

• 4 video sessions - each session is between 1- 2 hours long
• Tutor supported with access to our chromatography experts
• Supporting training material
• Course assessment with certificate

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HPLC column dimensions will affect the efficiency sensitivity, and speed of an analysis. The choice of column dimensions will depend on the chromatographic application; analytical, semi-preparative, preparative, number of analytes in the mixture etc.  However, the dimensions can also be altered to improve chromatography by achieving more efficient, sensitive, and faster analyses.  The primary column dimensions of particle size, length, and internal diameter will be discussed and their effect on chromatographic separations demonstrated, providing some practical insight into how to select the best column dimensions for a particular application...

HPLC Column Dimensions »

Headspace sampling is excellent for the qualitative or quantitative analysis of volatile species in samples that can be efficiently partitioned into the headspace gas volume from either a liquid or solid matrix.  It is also a good technique for the analysis of samples where the entire sample should not be injected into the GC instrument (i.e. dirty samples).  Headspace sampling is also particularly amenable to the analysis of trace levels of analytes.  

Typical examples of headspace analyses include...

View Headspace GC Quick Guide »