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Whether you are analyzing small or large molecules, column selection is one of the most important decisions during method development. However, no matter what size of molecule you are analyzing all of the parameters that need to be selected for a column are the same - there really are only so many parameters related to an HPLC column. There are just a few particular requirements related to using HPLC columns with large biomolecules. Therefore, we are going to walk you through selecting a reversed phase HPLC column for analyzing biomolecules.