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The correct setup of any instrument, method, or process in the lab is crucial to it performing to the best of its ability. Autosamplers are an invaluable part of chromatographic instrumentation as they are reliable and accurate and remove many of the errors associated with manual injection. However, we still need to take the time to set them up correctly. Over four short videos we will look at how to correctly setup a GC autosampler. We will consider parameters that can be changed by the user, including the syringe and needle, needle wash routines, vials, injection speed, viscosity delay, and dwell time.