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The most important thing in GC is to obtain the optimum resolution in the minimum time. Each of the important chromatographic parameters (resolution, retention factor, selectivity, efficiency and asymmetry) are explained and illustrated with real life examples in this module. 

At the end of this module you will be able to estimate resolution between peaks within a chromatogram; explain how resolution is influenced by retention factor, selectivity, and efficiency; recognize which of these factors should be improved to increase resolution within specific separations; develop strategies to efficiently optimise resolution – using the other chromatographic parameters; suggest ways to carry out optimisation and troubleshooting of separations in a practical context.


Topics include:

  • Chromatographic resolution and the resolution equation
  • Retention factor and the effect of retention factor on selectivity
  • How to change selectivity
  • Efficiency and how to improve efficiency
  • Real life examples of resolution
  • Peak asymmetry