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Proteomics is the large scale study of proteins, particularly their structures and functions. Mass spectroscopy provides a means of getting information relevant to the primary structure of a polypeptide or a protein, especially when fragmentation is induced using in-source collision induced dissociation (CID) or in the reaction cell of a triple quadrupole mass spectrometer (usually termed MS/MS). Protein and peptide sequencing by MS is not simple because various covalent bonds may be broken during fragmentation, resulting in a situation in which we must rebuild the intact protein structure which is typically done using powerful data analysis software. The purpose of this module is to introduce the ways in which mass spectrometry is used in proteomics research.

 

Topics include:

  • Properties of amino acids, proteins and peptides
  • MS structure determination
  • Electrospray (ESI) considerations and applications
  • MALDI ionization techniques and practical considerations
  • Peptide charging process and its importance for protein sequencing