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Available to following accounts
Academic
Premier

Selection of mobile phase and correct preparation is essential for a robust HPLC method. This webcast will provide you with a fail safe 20 step guide to the preparing the optimum mobile phase; including controlling pH, eluent mixing, filtration and degassing, mobile phase shelf life, and some of the most common mistakes to avoid.

 

Topics include:

  • pH – correctly controlling pH using buffers
  • Eluent mixing
  • Filtration and degassing
  • Reagent quality
  • Mobile phase shelf life
  • Impact of mobile phase in gradient HPLC
  • System flushing
  • Common mistakes to avoid