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Mobile Phase Optimization Strategies for Reversed Phase HPLC

Thursday, February 15th, 2024
8am PST | 11am EST | 4pm GMT | 5pm CET

This webcast will look at strategies for optimizing mobile phase composition in order to achieve the required resolution in the minimum amount of time. This is, of course, the primary goal of everyone undertaking method development. The chemistry of the aqueous and organic portions of the mobile phase will be studied, and we will discuss how to use these components to affect retention and improve the selectivity and resolution within our separations.


Topics include: 

  • Review of reversed phase retention mechanisms
  • Methanol or acetonitrile - which one is best?
  • Eluotropic strength - quick ways to reach the ideal
  • Changing solvent - useful tools and approaches
  • Which buffer, what strength?
  • Mobile phase pH - understand the effects
  • Optimizing pH vs. retention
  • Strategies for when all else fails 


Prof. Kevin Schug (Department of Chemistry and Biochemistry, The University of Texas at Arlington)

Tony Taylor (Chief Scientific Officer, Element Life Sciences EMEAA, Element Materials Technology)

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