HPLC Column Chemistry

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HPLC Column Chemistry

The aim of this module is to introduce silica as a major stationary phase support for reversed phase HPLC separations and for normal phase applications. We explain how bonded phases are produced and highlight the deactivation process used. The use of bonded phases in various chromatographic applications, and the influence of surface silica silanol groups on chromatographic separations is illustrated. Modern bonded phases that give an advantage to today’s chromatographer are introduced – including water wettable phases, polar embedded phases, and phases capable of operating at extremes of pH.

At the end of this section you will be able to highlight new and emerging stationary phase types, as well as explain how columns and stationary phases may be characterized.

Topics include:

Silica as a packing material
Chemically bonded phases
Surface treatment- endcapping
Reversed phase stationary phases
Silanol and separation
Water wettable phases
Aq type stationary phases
Polar embedded phases
Working at low pH
Working at high pH
Other stationary phase types
Column test probes
Column characterization

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