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Mobile Phase Optimization Strategies for Reversed Phase HPLC

This webcast will look at strategies for optimizing mobile phase composition in order to achieve the required resolution in the minimum amount of time. This is, of course, the primary goal of everyone undertaking method development. The chemistry of the aqueous and organic portions of the mobile phase will be studied, and we will discuss how to use these components to affect retention and improve the selectivity and resolution within our separations.


Topics include: 

  • Review of reversed phase retention mechanisms
  • Methanol or acetonitrile - which one is best?
  • Eluotropic strength - quick ways to reach the ideal
  • Changing solvent - useful tools and approaches
  • Which buffer, what strength?
  • Mobile phase pH - understand the effects
  • Optimizing pH vs. retention
  • Strategies for when all else fails 

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